Sequazyme standards kit

Manufactured by AB Sciex

The Sequazyme standards kit is a set of laboratory standards designed for the calibration and validation of mass spectrometry instruments. The kit contains a variety of reference compounds with known molecular weights and concentrations, which can be used to ensure the accuracy and precision of mass spectrometry analyses.

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Lab products found in correlation

Voyager de pro, by AB Sciex (2 mentions)

2 protocols using sequazyme standards kit

MALDI-TOF Mass Spectrometry of Polymers

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Mass spectra were acquired in
the reflectron mode using a Voyager-DE PRO (AB Sciex, Framingham,
MA) equipped with a nitrogen laser emitting at 337 nm. Ions were accelerated
to a 20 kV final potential. Positive ions were detected, and mass
spectra were a sum of 300 shots. External mass calibration was provided
by a mixture of peptides (Sequazyme standards kit, AB Sciex). Samples
were prepared by dissolving polymers in THF at a 10 g L^–1concentration. The matrix, 3-indoleacrylic acid (IAA; Sigma-Aldrich,
used without further purification), was dissolved in THF at 0.25 M.
Matrix and polymer solutions were mixed at a 9:1 (v/v) ratio, and
then 1 μL of the mixture was deposited and dried onto the MALDI
sample plate.

Berki T., Bakunts A., Duret D., Fabre L., Ladavière C., Orsi A., Charreyre M.T., Raimondi A., van Anken E, & Favier A. (2019). Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology. ACS Omega, 4(7), 12841-12847.

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MALDI-TOF Mass Spectrometry of Proteins

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Mass spectra were acquired with a Voyager-DE PRO (Sciex, Framingham, MA, USA) equipped with a nitrogen laser emitting at 337 nm. Ions were accelerated to a final potential of 20 kV, and the mass spectrum was the sum of 300 laser shots. An external mass calibration was used (a mixture of peptides from the Sequazyme™ standards kit, AB Sciex). The analysis was performed in linear mode (instrumental mass accuracy is 0.05%). Samples were prepared by diluting 10-fold the protein solution (0.2 mg/ml) in the matrix sinapinic acid (Sigma-Aldrich, St. Louis, MI, USA), used without further purification and dissolved in 0.1%TFA/acetonitrile (70/30 v/v). About 1 μl of the mixture was deposited onto the MALDI sample plate and let dried to complete co-crystallization.

Fogeron M.L., Lecoq L., Cole L., Montserret R., David G., Page A., Delolme F., Nassal M, & Böckmann A. (2022). Phosphorylation of the Hepatitis B Virus Large Envelope Protein. Frontiers in Molecular Biosciences, 8, 821755.

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