Hek293t cell line

The chronic myeloid leukemia cell line K562 (RRID:CVCL_0004; ordered from DSMZ, Braunschweig, Germany) and BL cell line ST486 (RRID:CVCL_1712; ATCC, Manassas, VA, USA) were maintained in Roswell Park Memorial Institute 1640 medium (RPMI, Lonza, Basel, Switzerland) supplemented with 10–20% fetal bovine serum (Sigma‐Aldrich, Saint Louis, MO, USA), 2 mm l‐glutamine (Biowest, Nuaille, France), and 1% penicillin/streptomycin (Biowest) in a 5% CO₂ incubator at 37 °C. Hepatocellular carcinoma cell line HepG2 (RRID:CVCL_0027; DSMZ), breast cancer cell line MCF7 (RRID:CVCL_0031; ECACC, Porton Down, UK), and HEK293T cell line (RRID:CVCL_0063; DSMZ) used for lentiviral particle production were cultured in low glucose Dulbecco's Modified Eagle's Medium (DMEM, Lonza) supplemented as described above. In addition, medium for MCF7 cells was supplemented with 1× NEAA (Gibco, Waltham, MA, USA). Cell lines were authenticated by providers in the period of 3 years before the experiments. They were expanded and banked in our laboratory immediately upon receipt. After thawing, cells were cultured for experiments no longer than 3 months. Mycoplasma tests were routinely performed and confirmed that the cells were not contaminated.