2020 lc ms instrument

¹H and ¹³C NMR were recorded on a Bruker 400 spectrometer. ¹H NMR data are reported as follows: chemical shift in ppm (δ), multiplicity (s = singlet, d = doublet, t = triplet, m = multiplet), coupling constant (Hz), relative intensity. ¹³C NMR data are reported as follows: chemical shift in ppm (δ). LC/MS analyses were performed on a Shimadzu-2020 LC-MS instrument using the following conditions: Shim-pack VP-ODS C18 column (reverse phase, 150 × 4.6 mm); a linear gradient from 10% water and 90% acetonitrile to 75% acetonitrile and 25% water over 6.0 min; flow rate of 0.5 mL/min; UV photodiode array detection from 200 to 400 nm. High-resolution mass spectra (HRMS) were recorded on a Q Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific) with an ESI source of 140,000 fwhm, AGC target set to 1 × 10⁶, and a scan range of 100–1000 m/z. The raw data were deconvoluted using an Xcalibur 4.1. UV-VIS spectrophotometer TU-1950. The products were purified by Biotage Isolera™ Spektra Systems and hexane/EtOAc solvent systems. All reagents and solvents were obtained from commercial sources and used without further purification.

¹H and ¹³C NMR data were recorded on a Bruker (Billerica, MA, USA) 400 spectrometer. ¹H NMR data are reported as follows: chemical shift in ppm (δ), multiplicity (s = singlet, d = doublet, t = triplet, m = multiplet), coupling constant (Hz), relative intensity. ¹³C NMR data are reported as follows: chemical shift in ppm (δ). HPLC-MS analyses were performed on a Shimadzu-2020 LC-MS instrument (Kyoto City, Japan) using the following conditions: Shim-pack VPODS C18 column (reverse phase, 150 × 2.0 mm); 80% acetonitrile and 20% water over 6.0 min; flow rate of 0.4 mL/min; UV photodiode array detection from 200 to 300 nm. The products were purified using Biotage (Uppsala, Sweden) Isolera™ Spektra Systems and hexane/EtOAc solvent systems. Unless otherwise specified, all chemicals were purchased from commercial sources and used without further treatment. All microwave irradiation experiments were carried out according to our previous report [44 (link),45 (link)].