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Gemini rp c18 column

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The Gemini RP-C18 column is a reverse-phase liquid chromatography column. It is designed for the separation and analysis of a wide range of chemical compounds. The column features a C18 stationary phase and is suitable for use in various analytical applications.

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Lab products found in correlation

Lipofectamine ltx and plus reagent, by Thermo Fisher Scientific (1 mentions) Kieselgel 60, by Merck Group (1 mentions) Theobromine, by Merck Group (1 mentions) Spd m20a, by Shimadzu (1 mentions)

Close spelling variants of this product

C18 column (366 citations) Gemini C18 (326 citations) Gemini C18 column (288 citations) RP-C18 column (22 citations) Gemini column (17 citations) RP C18 (5 citations) Gemini RP-C18 (3 citations) RP Gemini C18-110A preparative column (3 citations)

4 protocols using gemini rp c18 column

1

Azido Nucleoside Synthesis and Characterization

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Detailed methods and characterization can be found in the Supporting Information. The 1H (400 MHz), 19F NMR (376.4 MHz) and 13C (100.6 MHz) were recorded at ambient temperature in solutions of ACN-d3, D2O, or DMSO-d6, as noted. The reactions were followed by TLC with Merck Kieselgel 60-F254 sheets, and products were detected with a 254 nm light. Column chromatography was performed using Merck Kieselgel 60 (230–400 mesh). Reagent-grade chemicals were used. Azido nucleoside substrates were prepared as described in literature (1,59 (link)2,61 4,62 and 2270 (link)) or in SI section (3), or are commercially available (19, 21 and 28) from Carbosynth. Cyclooctynes 5, 6, 12 and 16 are commercially available from Kerafast, SynAffix, Sigma Aldrich or Berry & Associates. Lipofectamine LTX and Plus reagent was purchased from Invitrogen. HPLC analysis was performed on a semi-preparative Phenomenex Gemini RP-C18 column (5 μ, 25 cm × 1 cm) with UV detection at 254 nm.
Zayas J., Annoual M., Das J.K., Felty Q., Gonzalez W.G., Miksovska J., Sharifai N., Chiba A, & Wnuk S.F. (2015). Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application to Living Cell Fluorescent Imaging. Bioconjugate chemistry, 26(8), 1519-1532.
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2

Synthesis and Purification of Cyclooctyne-Substituted Compound

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Cyclooctyne 12 (5.72 mg, 0.02 mmol) was added to a stirred solution of 2 (4.0 mg, 0.02 mmol) in MeOH (1 mL) at 50 °C. After 16 h, the crude reaction mixture was passed through a 0.2 μm PTFE syringe filter, and then injected into a semipreparative HPLC column (Phenomenex Gemini RP-C18 column; 5 μ, 25 cm × 1 cm) (40% ACN/H2O, 1.5 mL/min) to give 14 (8.52 mg, 95%) as a mixture of several inseparable regioisomers (tR = 5.5–10.0 min): 1H NMR (DMSO-d6) δ Two major isomers (~85–90% of total isomeric composition) had: 1H NMR (DMSO-d6) δ 1.42–1.78 (m, 2H, NH2), 2.01–2.17 (m, 2H, CH2CO), 2.60–2.78 (m, 2H, CH2NH2), 3.17–3.24 (m, 2H, H4′ & H5′), 3.28 (dd, J = 11.5 Hz, 4.6 Hz, 1H, H5″), 3.83–3.87 (m, 1H, H3′), 4.10 (t, J = 6.0 Hz, 0.5H, H2′), 4.11 (t, J = 5.9 Hz, 0.5H, H2′), 4.49 (br s, 2H, CH2), 5.72 (d, J = 5.1 Hz, 1H, H1′), 7.26–7.56 (m, 8H, Har), 8.09 (s, 1H, H2); HRMS (ESI+) m/z calcd C28H29N10O5 (M+H)+: 585.2317, found: 585.2425.
Zayas J., Annoual M., Das J.K., Felty Q., Gonzalez W.G., Miksovska J., Sharifai N., Chiba A, & Wnuk S.F. (2015). Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application to Living Cell Fluorescent Imaging. Bioconjugate chemistry, 26(8), 1519-1532.
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3

Synthesis and Purification of Cyclooctyne Derivative

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Cyclooctyne 12 (4.7 mg, 0.015 mmol) was added to a stirred solution of 4 (4.9 mg, 0.015 mmol) in MeOH (1.5 mL) at 50 °C. After 16 h, the crude reaction mixture was passed through a 0.2 μm PTFE syringe filter, and then injected into a semipreparative HPLC column (Phenomenex Gemini RP-C18 column; 5 μ, 25 cm × 1 cm (40% ACN/H2O, 2.0 mL/min) to give 15 (9.5 mg, 95%) as a mixture of several inseparable regioisomers (tR = 8.5–5.0 min): UV λmax 273 nm (ε 12 550); 1H NMR (DMSO-d6) δ 1.64–1.81 (m, 2H, NH2), 2.11–2.15 (m, 1H, H2′), 2.15–2.30 (m, 2H, CH2CO), 2.60–2.79 (m, 2H, CH2NH2), 3.34–3.95 (m, 4H, H3′, H4′, H5′ & H5″), 4.51–4.60 (m, 2H, CH2), 4.91 (dd, J = 5.8, 2.3 Hz, 1H, H2′), 5.18–5.48 (m, 3H, 3 x OH), 5.74 (d, J = 6.7 Hz, 0.5H, H1′), 5.76 (d, J = 6.2 Hz, 0.5H, H1′), 5.94 (br d, J = 15.8 Hz, 1H, CH2), 6.12 (br d, J = 16.4 Hz, 1H, CH2), (6.86–7.84 (m, 8H, Har), 8.4 (s, 0.5H, H2), 7.94 (s, 0.5H, H2); HRMS (ESI+) m/z calcd C28H29N10O4 (M+H)+: 569.2368, found: 569.2411.
Zayas J., Annoual M., Das J.K., Felty Q., Gonzalez W.G., Miksovska J., Sharifai N., Chiba A, & Wnuk S.F. (2015). Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and 8-Azidoadenosine Triphosphate with Cyclooctynes. Application to Living Cell Fluorescent Imaging. Bioconjugate chemistry, 26(8), 1519-1532.
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4

HPLC Analysis of Methylxanthines in Ilex paraguariensis

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An HPLC Shimadzu Prominence 20AT module (Kyoto, Japan) coupled to a photodiode array detector (PDA) SPD-M20A, controlled by LC-Solution Multi-PDA software, was used. A Gemini RP C18 column (Phenomenex, 250 × 4.6 mm i.d.; 5 μm particle size) coupled with a C18 guard column was used as the stationary phase.
Methylxanthines were assayed by HPLC based on a previously validated method employing caffeine and theobromine as external standards.15 (link) The theobromine (Sigma-Aldrich, St. Louis, MO, USA) and caffeine (Sigma-Aldrich) standards were properly dissolved in methanol: water 30/70 (v/v), at concentrations ranging from 0.48 to 40.0 μg/mL (caffeine) and from 0.495 to 7.005 μg/mL (theobromine). An isocratic system was employed, using methanol/water 30/70 (v/v) as the mobile phase. The flow rate (1.1 mL/min) and temperature (35 ± 1 °C) were kept constant throughout the analysis, which took 10 min. The detection was performed at 280 nm. All samples were properly diluted with methanol/water 30/70 (v/v) seeking the linearity range of standard curves. The total methylxanthine content was determined by the sum of caffeine and theobromine individual concentrations. A representative chromatogram of methylxanthine (caffeine and theobromine) dosage in lot 07/18 of the Ilex paraguariensis aqueous extract is shown in Additional file 1.
Rocha D.S., Argenta Model J.F., Von Dentz M., Maschio J., Ohlweiler R., Lima M.V., Khal de Souza S., Sarapio E., Vogt É.L., Waszczuk M., Martiny S., Bassani V.L, & Kucharski L.C. (2020). Adipose tissue of female Wistar rats respond to Ilex paraguariensis treatment after ovariectomy surgery. Journal of Traditional and Complementary Medicine, 11(3), 238-248.
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