Sod colorimetric activity kit

The study was designed into seven groups—the five sample groups listed in Table 1, the model or UV radiation group, and the control group. The control was cells incubated without any antioxidant or UV radiation treatment. The model group was cells irradiated with UVB (50 J/cm², 5 min) and continued to incubate. The detailed preparations of sample groups were as follows: the cells in the exponential growth phase were taken to make a single cell suspension, seeded on a 6‐well plate at a density of 1.5 × 10⁵ cells/mL, and were cultured in a medium containing 10% fetal bovine serum at 37°C for 24 h. The final volume was 2 mL, and the supernatant was discarded. The sample (Table 1) was prepared into a stock solution with DMSO or ddH₂O, and was diluted to the appropriate concentration with serum‐free medium or PBS. The freshly prepared samples were added and incubated with a serum‐free medium. After incubating for 24 h, the cells were irradiated with UVB (50 J/cm², 5 min) using a UV lamp, and continued to incubate at 37°C for 16 h. The activity of SOD was determined by using the SOD Colorimetric Activity Kit (Beyotime, Shanghai, China). In addition, the total cellular RNA was extracted, and qPCR was used to quantify SOD1, SOD2, SOD3 mRNA expression. NQO1 protein content was detected by Western Blot.