Sp8 x wll confocal microscope

Manufactured by Leica

The Leica SP8 X WLL Confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a white light laser source, enabling a wide range of excitation wavelengths, and a high-speed resonant scanner for fast image acquisition. The system is optimized for confocal imaging, providing optical sectioning capabilities and high-resolution visualization of samples.

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WLL Leica SP8 microscope SP8 WLL SP8 confocal microscope Confocal SP8 SP8 X SP8 microscope Confocal microscope

3 protocols using sp8 x wll confocal microscope

High-Resolution Confocal Imaging of Embryos

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All images were acquired using the Leica SP8 X WLL Confocal microscope using the 63x glycerol immersion lens, NA = 1.44. Z-stacks of fixed embryos were acquired at the system optimized Z-step, 512 × 512 resolution, 600 Hz, and bi-directional scanning. Multiple HyD detectors were used to capture images at various wavelengths.

Bernadskaya Y.Y., Brahmbhatt S., Gline S.E., Wang W, & Christiaen L. (2019). Discoidin-domain receptor coordinates cell-matrix adhesion and collective polarity in migratory cardiopharyngeal progenitors. Nature Communications, 10, 57.

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Confocal Imaging of Embryonic Samples

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All images were acquired using the Leica SP8 X WLL confocal microscope using the 63× glycerol immersion lens, NA = 1.44. Z-stacks of fixed embryos were acquired at the system optimized Z-step, 512 × 512 resolution, 600 Hz, and bidirectional scanning. Multiple HyD detectors were used to capture images at various wavelengths.

Bernadskaya Y.Y., Yue H., Copos C., Christiaen L, & Mogilner A. (2021). Supracellular organization confers directionality and mechanical potency to migrating pairs of cardiopharyngeal progenitor cells. eLife, 10, e70977.

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Confocal Imaging of Embryo Development

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All images were acquired using the Leica SP8 X WLL Confocal microscope using the 63x glycerol immersion lens, NA = 1.44. Z-stacks of fixed embryos were acquired at the system optimized Z-step, 512x512 resolution, 600 Hz, and bi-directional scanning. Multiple HyD detectors were used to capture images at various wavelengths.

Bernadskaya Y.Y., Yue H., Copos C., Christiaen L., & Mogilner A. (2021). Supracellular organization confers directionality and mechanical potency to migrating pairs of cardiopharyngeal progenitor cells.

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