Pulmonary microvascular permeability was measured using Evans Blue dye, as previously described [27 ]. Briefly, 200 μL of 0.5% Evans Blue dye (Nacalai Tesque, Inc. Kyoto, Japan) was injected intravenously 10 min prior to ADSC or PBS administration. Mice were injected with LY294002 and histones and euthanized at previously described time points. The lungs of sacrificed mice, which were perfused with 5 mL of PBS through their right ventricle and injected with histones after 1 h, was harvested, dried at 60 °C for 24 h, and then weighed. Evans Blue in the dried lungs was extracted by immersion in 3 mL of 100% formamide at 37 °C for 48 h. Evans Blue concentration in the supernatant was then determined by absorbance at 620 nm using a microplate reader (EnSpire 2300 Multilabel; PerkinElmer Inc., Waltham, MA, USA) and expressed as micrograms per gram of dry tissue weight.
Enspire 2300 multilabel
Manufactured by PerkinElmer
Sourced in United States
The EnSpire 2300 Multilabel is a high-performance plate reader designed for a wide range of detection modes, including absorbance, fluorescence, luminescence, and time-resolved fluorescence. It offers reliable and accurate measurements for various applications in life science research and drug discovery.
Automatically generated - may contain errors
Lab products found in correlation
Evans blue dye, by Nacalai Tesque (2 mentions)
Gssg gsh quantification kit, by Dojindo Laboratories (1 mentions)
Oxiselect total antioxidant capacity assay kit, by Cell Biolabs (1 mentions)
Sod assay kit wst, by Dojindo Laboratories (1 mentions)
Ifnγ mouse uncoated elisa kit, by Thermo Fisher Scientific (1 mentions)
5 protocols using enspire 2300 multilabel
1
Quantifying Pulmonary Microvascular Permeability
2
Antioxidant Profiling of Liver Tissue
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Liver tissues were homogenized in lysis buffer, centrifuged, and the supernatants were collected. The total antioxidant capacity (TAC), GSH, SOD and catalase activity in tissue homogenates were analysed using an OxiSelect Total Antioxidant Capacity Assay Kit (Cell Biolabs Inc., San Diego, CA, USA), a GSSG/GSH Quantification Kit (Dojindo Laboratories, Kumamoto, Japan), a SODAssay Kit-WST (DOJINDO) and a catalase colorimetric assay kit (ARBOR ASSAYS, Bangor, ME, USA), respectively, according to the standard protocols. The samples were analysed using the microplate reader EnSpire 2300 Multilabel (PerkinElmer Inc., Waltham, MA, USA).
3
Splenocyte-B16 Coculture IFNγ Assay
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A total of 5×105 splenocytes were cocultured with 5×104 B16 cells for 24 hours or 48 hours. Supernatants were collected from triplicate wells, and IFNγ was assayed using the mouse uncoated IFNγ ELISA kit from Invitrogen (Carlsbad, California, USA). Readings were measured at 450 nm using the EnSpire 2300 Multilabel plate reader (Perkin Elmer, Waltham, Massachusetts, US).
4
Pulmonary Microvascular Permeability Assay
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Pulmonary microvascular permeability was measured using Evans Blue dye, as previously described [27] . Brie y, 200 µL of 0.5% Evans Blue dye (Nacalai Tesque, Inc. Kyoto, Japan) was injected intravenously 10 min prior to ADSC or PBS administration. Mice were injected with LY294002 and histones and euthanized at previously described time points. The lung of sacri ced mice, which were perfused with 5 mL of PBS through their right ventricle and injected with histones after 1 h, was harvested, dried at 60 °C for 24 h, and then weighed. Evans Blue in the dried lungs was extracted by immersion in 3 mL of 100% formamide at 37 °C for 48 h. Evans Blue concentration in the supernatant was then determined by absorbance at 620 nm using a microplate reader (EnSpire 2300 Multilabel; PerkinElmer Inc., Waltham, MA, USA) and expressed as µg/g of dry tissue weight.
5
Quantifying Pulmonary Microvascular Permeability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pulmonary microvascular permeability was measured using Evans Blue dye, as previously described [27] . Brie y, 200 μL of 0.5% Evans Blue dye (Nacalai Tesque, Inc. Kyoto, Japan) was injected intravenously 10 min prior to ADSC or PBS administration. Mice were injected with LY294002 and histones and euthanized at previously described time points. The lungs of sacri ced mice, which were perfused with 5 mL of PBS through their right ventricle and injected with histones after 1 h, was harvested, dried at 60 °C for 24 h, and then weighed. Evans Blue in the dried lungs was extracted by immersion in 3 mL of 100% formamide at 37 °C for 48 h. Evans Blue concentration in the supernatant was then determined by absorbance at 620 nm using a microplate reader (EnSpire 2300 Multilabel; PerkinElmer Inc., Waltham, MA, USA) and expressed as μg/g of dry tissue weight.
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