Anti il 10 fitc

Manufactured by Thermo Fisher Scientific

Anti-IL-10-FITC is a fluorescently-labeled antibody designed to detect the cytokine interleukin-10 (IL-10) in various research and diagnostic applications. The FITC (fluorescein isothiocyanate) label allows for the visualization and quantification of IL-10 expression using flow cytometry or other fluorescence-based detection methods.

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2 protocols using anti il 10 fitc

Characterizing Immune Cell Populations in Mouse Spleen

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Spleen tissues were obtained from mice at 15–20 weeks after primary immunization, and the B- and T-cell populations of interest were identified using the following specific antibodies (eBioscience): anti-B220-APC, anti-GL7-FITC (or PerCP), anti-CXCR5-PerCP, anti-CD138-PE, anti-ICOS-PE (or APC), anti-IgD-FITC, anti-IgM-PE, anti-CD19-PE (or PerCP), anti-CD5-FITC, anti-IL-10-FITC, anti-Foxp3-PE, anti-CD4-PerCP (or FITC, PE), anti-CD25-APC, anti-Foxp3-FITC (or APC), anti-IL-17-PE, anti-IL-4-PE (or FITC), and anti-IFN-γ-FITC (or PE). Stained sections were visualized by confocal microscopy (LSM 510 Meta, Carl Zeiss, Oberkochen, Germany). The expression of GC or plasma B cells were estimated by comparing the mean fluorescence intensity using LSM 510 Meta, Carl Zeiss software.

Lee S.Y., Lee S.H., Seo H.B., Ryu J.G., Jung K., Choi J.W., Jhun J., Park J.S., Kwon J.Y., Kwok S.K., Youn J., Park S.H, & Cho M.L. (2019). Inhibition of IL-17 ameliorates systemic lupus erythematosus in Roquinsan/san mice through regulating the balance of TFH cells, GC B cells, Treg and Breg. Scientific Reports, 9, 5227.

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Multiparameter Flow Cytometry Assay

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All cells were assessed for viability using a fixable viability dye (ebioscience eFluor506). Flow cytometric analysis of human and murine cells was performed using >50 antibodies and appropriate directly conjugated mouse, rat and hamster isotype controls. The clones . relevant to the majority of work are as follows: mouse anti-CD3-FITC (Miltenyi; REA641) anti-CD8a-PerCP (Miltenyi; 53-6.7) anti-CD28-PE (Miltenyi; 37.51) anti-KLRG1-APC (Miltenyi; 2F1). Human anti-CD3 APC-Cy7 (biolegend; HIT3a) anti-CD8-PE-Vio770 (Miltenyi; REA734) anti-CD28-eFluor450 (ebioscience; CD28.2) anti-KLRG1-AF488 (a generous gift from Professor Hanspeter Pircher, University of Freiburg) anti-CD57-Vioblue (Miltenyi; TB03) anti-CD244-FITC (biolegend; C1.7) anti-CX3CR1-PE (ebioscience; 2A9-1), and anti-IL-10-FITC (ebioscience; BT-10). For intracellular cytokine staining, cells were cultured with PMA (50 ng/ml), ionomycin (500 ng/ml), and monensin (3 µM; all from Sigma) for 4 h at 37C. Cells were stained for cell surface markers, then fixed and permeabilized in Cytofix/Cytoperm (BD) before intracellular detection of cytokines. Cells were acquired using a CyAn ADP (Beckman Coulter) flow cytometer and analysed using Summit software (software version 4.3; Beckman Coulter). Percentages and mean fluorescence intensity (MFI) were calculated against appropriate isotype controls.

Thompson C., Beatson R., Davies R., Greenhill C., Jones S.A., Williams A.S., Jones G.W., & Choy E. (2020). Peripheral regulatory CD8⁺CD28⁻KLRG1⁺ T cells as markers of disease and treatment response in rheumatoid arthritis.

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