Dimethylsulfoxide

Chitosan MW 583 kDa (78% deacetylated), glucosamine hydrochloride (GAHC), nitrotetrazolium blue chloride (NBT), hydroxylamine hydrochloride (HAHC), 2-Deoxy-Dribose (DoR), methionine, riboflavin, trichloroacetic acid (TCA), p-nitrophenyl-2acetamido-β-glucopyranoside, citric acid, hexadecyl trimethyl ammonium bromide, sodium tripolyphosphate (TPP), aglycone Genistein (Ge) and fluorescein isothiocyanate (FITC) were obtained from Sigma-Aldrich (MO). Thiobarbituric acid (TBA) and ethylenediaminetetraacetic (EDTA) were provided by Merck (Germany); monobasic potassium phosphate (KH 2 PO 4 ), sodium chloride (NaCl), sodium hydroxide (NaOH), potassium chloride (KCl), sodium bicarbonate (NaHCO 3 ), ascorbic acid, hydrogen peroxide (H 2 O 2 ), hydrochloride acid (HCl), ferric chloride (FeCl 3 ), and glacial acetic acid (CH 3 COOH) were from Cicarelli (Argentina). Dimethyl sulfoxide (DMSO) was from Biopack (Argentina). Bradford reagent was from Bio-Rad (USA) and 3,3′,5,5′ tetramethylbenzidine (TMB) was provided by BD Biosciences (USA). Dextran sulfate sodium (DSS) was kindly provided by Dextran Products Limited (Canada). Aqueous solutions were prepared with ultrapure water.

Poly(allylamine hydrochloride), 58 kDa, sodium chloride, 4-(2hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES), phosphate-buffered saline (PBS, D1408), fibronectin (FN) from bovine plasma (F4759), sodium dodecyl sulfate (SDS, L6026), Triton X-100 (T8787), Tween-20 (P9416) and fibronectin from bovine plasma (F4759) were purchased from Sigma-Aldrich. KH 2 PO 4 (Pi) was supplied by Biopack (Argentina), RPMI 1640 with L-glutamine was purchased from Fisher, minimum essential medium (MEM) was acquired from Sigma-Aldrich and fetal bovine serum (FBS) from Natacor (Argentina). The reagent MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) was provided by Thermo Fisher Scientific, and dimethyl sulfoxide by Biopack (Argentina). Deionized water was obtained using the Milli-Q water purification system.