Anti cleaved poly adp ribose polymerase 1 parp1 asp214 d64e10

Anti-FLAG M2 and anti-β-actin antibodies were purchased from Sigma-Aldrich Chemicals. Anti-p21 waf1 , anti-cyclin D1, anti-p53, anti-BCL2, and anti-Ki-67 antibodies were obtained from Dako (Copenhagen, Denmark). Anti-p27 kip1 and anti-BAX antibodies were from BD Biosciences (San Jose, CA, USA). Anti-MDM2 and anti-S100A1 were from Abcam (Cambridge, MA, USA). Anti-cleaved caspase-3 and anticleaved poly (ADP-ribose) polymerase 1 (PARP1)(Asp214)(D64E10) were from Cell Signaling (Danvers, MA, USA). Anti-cyclin B1 and anti-cyclin A2 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Novocastra (Newcastle, UK), respectively. Rapamycin, aphidicolin, and nocodazole for synchronization of cells at G1, early S, and G2/M phases, respectively, were obtained from Calbiochem (Cambridge, MA, USA). Adriamycin (ADR) and Nutlin-3A were from Sigma-Aldrich Chemicals.
Reverse transcription (RT)-PCR cDNA was synthesized from 2 µg of total RNA. Ampli cation by RT-PCR was carried out in the exponential phase to allow comparison among cDNA synthesized from identical reactions using speci c primers (Table 1). Primers for the GAPDH gene were also used as described previously [13, 16] .