Sybr green kit

Manufactured by Genetix

Sourced in United States

The SYBR Green kit is a reagent used for quantitative real-time PCR (qPCR) experiments. It binds to double-stranded DNA and emits a fluorescent signal, allowing for the detection and quantification of DNA amplification during the PCR process.

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Lab products found in correlation

Complementary dna cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Antibiotic cocktail, by Merck Group (1 mentions) Dimethyl methylene blue dmmb, by Merck Group (1 mentions) 1 1 diphenyl 2 picrylhydrazyl radical, by Merck Group (1 mentions)

2 protocols using sybr green kit

Chondrocyte Culture and Analysis

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Cell culture media and supplements such as Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) Media, Fetal bovine serum (FBS), antibiotic cocktail, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), safranin-o and toluidine blue dye, 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), 2′-azinobis [3-ethylbenzthiazoline]-6-sulfonic acid (ABTS), and Dimethyl methylene blue (DMMB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Complementary DNA (cDNA) synthesis kit was purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA) and SYBR Green kit was obtained from Genetix (New Delhi, India). Rat CTX-II (ELISA KIT E-EL-R2554) and Rat COMP (ELISA kit E-EL-R0159) were purchased from Elabsciences (Elabsciences biotechnology co ltd, Hubei province, China).

Choudhary D., Kothari P., Tripathi A.K., Singh S., Adhikary S., Ahmad N., Kumar S., Dev K., Mishra V.K., Shukla S., Maurya R., Mishra P.R, & Trivedi R. (2018). Spinacia oleracea extract attenuates disease progression and sub-chondral bone changes in monosodium iodoacetate-induced osteoarthritis in rats. BMC Complementary and Alternative Medicine, 18, 69.

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Cell Viability and Oxidative Stress Assays

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Cell culture media and supplements such as Modified Eagle Medium, Fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-Dichlorofluorescindiacetate (DCFDA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Complementary DNA (cDNA) synthesis kit was purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA) and SYBR Green kit was obtained from Genetix (New Delhi, India).

Trivedi R., Adhikary S., Kothari P., Ahmad N., Mittapelly N., Pandey G., Shukla M., Kumar S., Dev K., Choudhary D., Maurya R., Lal J., & Mishra P.R. (2020). Self-emulsifying formulation of Spinacia oleracea reduces the dose and escalates bioavailability of bioactive compounds to accelerate fracture repair in rats. Clinical Phytoscience, 6(1).

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