Proteins from corneal epithelial samples were extracted using the PARIS kit (Thermo Fisher). Total protein concentration was measured using DC protein assay kit (500–0112, Bio-Rad). 20 μg protein from cell lysates was subjected to 7.5% SDS-PAGE and transferred to Hybond ECL nitrocellulose membrane (Amersham Biosciences, Piscataway, NJ). After incubation with appropriate primary and secondary antibodies, the blots were detected with the Supersignal Femto Chemiluminescent Substrates (ThermoFisher, MA, USA). For re-probing, blots were washed in Western blot stripping buffer (Thermo Fisher) for 15 min before proceeding with new blotting. Anti-ACE2 (ab108252, 1:1000) and anti-TMPRSS2 (ab92323, 1:1000) antibodies were purchased from Abcam. β-actin detected by its antibody (#4970, 1:2000, Cell Signaling) was used for loading control. Horseradish peroxidase-tagged secondary anti-rabbit IgG was from Cell Signaling (#7074, 1:2000).
Anti ace2 ab108252
Manufactured by Abcam
Anti-ACE2 (ab108252) is a recombinant antibody that recognizes the angiotensin-converting enzyme 2 (ACE2) protein. ACE2 is a key receptor for the SARS-CoV-2 virus, which causes COVID-19. This antibody can be used to detect and study the ACE2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal femto chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
Dc protein assay kit, by Bio-Rad (2 mentions)
Western blot stripping buffer, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase tagged secondary anti rabbit igg, by Cell Signaling Technology (2 mentions)
Hybond, by Cytiva (2 mentions)
Paris kit, by Thermo Fisher Scientific (1 mentions)
Anti tmprss2 ab92323, by Abcam (1 mentions)
Hybond ecl nitrocellulose membrane, by Cytiva (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
2 protocols using anti ace2 ab108252
1
Western Blot Analysis of ACE2 and TMPRSS2
2
Western Blot Analysis of ACE2 Protein
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Proteins from HRECS and HRPs were extracted using RIPA lysis buffer (Sigma-Aldrich Corp., St. Louis, MO, USA). Total protein concentration was measured using DC protein assay kit (500-0112; Bio-Rad Life Science, Hercules, CA, USA). Protein 80 µg from cell lysates was subjected to 7.5% SDS-PAGE and transferred to Hybond ECL nitrocellulose membrane (Amersham Biosciences, Piscataway, NJ, USA). After incubation with appropriate primary and secondary antibodies, the blots were detected with the Supersignal Femto Chemiluminescent Substrates (Thermo Fisher Scientific, Waltham, MA, USA). For reprobing, blots were washed in Western blot stripping buffer (Thermo Fisher Scientific) for 15 minutes before proceeding with new blotting. Anti-ACE2 (ab108252, 1:1000) antibodies were purchased from Abcam. The β-actin detected by its antibody (no. 4970, 1:2000; Cell Signaling) was used for loading control. Horseradish peroxidase-tagged secondary anti-rabbit IgG was from Cell Signaling (no. 7074, 1:2000).
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