Cells in culture were fixed using 4% PFA. For the IHC analysis of cells transplanted in the mouse brain, mice at 1, 3 or 6 months post-transplantation were perfused with 4% FPA through transcardial route. 100 µm-thick brain sagittal slices were sliced with Cryostat tissue slice instrument (Leica) after cryopreservation in 30% sucrose. Immunostainings were performed using antibodies listed in Table S4. For analyses of Arc expression by IHC, a rabbit anti-FMRP antibody (Cell Signaling Technology 7104) was labeled with Alexa fluor 555 fluorophore using an antibody labeling kit (Thermo Fisher Scientific A20187). Images were acquired using a Zeiss LSM700 confocal microscope. Antibodies used in this study are listed in Table S2.
Cryostat tissue slice instrument
Manufactured by Leica
The Cryostat tissue slice instrument is a specialized laboratory equipment used for preparing thin tissue samples for microscopic examination. It is designed to maintain the sample at a low, controlled temperature, allowing for the precise sectioning of frozen tissue specimens.
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Lab products found in correlation
2 protocols using cryostat tissue slice instrument
1
Immunohistochemistry of Transplanted Cells
2
Immunohistochemistry of Transplanted Cells
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Cells in culture were fixed using 4% PFA. For the IHC analysis of cells transplanted in the mouse brain, mice at 1, 3 or 6 months post-transplantation were perfused with 4% FPA through transcardial route. 100 µm-thick brain sagittal slices were sliced with Cryostat tissue slice instrument (Leica) after cryopreservation in 30% sucrose. Immunostainings were performed using antibodies listed in Table S4. For analyses of Arc expression by IHC, a rabbit anti-FMRP antibody (Cell Signaling Technology 7104) was labeled with Alexa fluor 555 fluorophore using an antibody labeling kit (Thermo Fisher Scientific A20187). Images were acquired using a Zeiss LSM700 confocal microscope. Antibodies used in this study are listed in Table S2.
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