Male balb c

Male BALB/c (haplotype: H-2^d), C57BL/6J (B6) (H-2^b), CBA/N (H-2^k), 129X1/Sv (H-2^b), C57BL/10 (B10) (H-2^b), and B6D2F1 (H-2^b/d), and female C3H/He (H-2^k) and B6 mice (5–8 weeks old) were purchased from Japan SLC, Inc. (Shizuoka, Japan). All animal procedures were approved by the Hokkaido University Animal Care Committee (Approval number: 17–0110) and the eperiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health.

Male BALB/c and C57BL/6 mice were purchased from the Japan SLC Corporation (Hamamatsu, Japan). CX3CR1 homogenous knockout in C57BL/6 background mice was performed by KAN Research Institute (Kobe, Japan). All mice were bred and maintained under specific pathogen-free conditions at the Institute of Laboratory at Tokyo Women’s Medical University (Tokyo, Japan). The pathogen-free conditions implemented were based on the criteria of the Central Institute for Experimental Animals (Kawasaki, Japan). The Tokyo Women’s Medical University internal committee on the use and care of laboratory animals approved all experiments (Reference ID: AE19-081).