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Ha mms 101r 50

Manufactured by Fortrea
Sourced in United Kingdom

The HA (MMS-101R-50) is a laboratory equipment product designed for various scientific applications. It serves as a high-performance analytical tool, facilitating the measurement and analysis of specific parameters within a research or testing environment. The core function of this product is to provide accurate and reliable data, enabling researchers and scientists to make informed decisions in their respective fields of study.

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2 protocols using ha mms 101r 50

1

Immunofluorescence and Immunogold Labeling

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Antibodies against reticulon 4B (AB-163; Kinasource Ltd, Dundee, UK), reticulon 4A (ab62024; Abcam, Cambridge, UK), HA (MMS-101R-50; Covance, Princeton, NJ), FLAG (F7425; Sigma-Aldrich), calreticulin (2679S; Cell Signalling Technologies, MA) and β-actin (ab8227-50; Abcam) were used as primary antibodies. When indicated, rabbit anti-sheep bridging antibody (313-001-003; Jackson ImmunoResearch Labs Inc., West Grove, PA) was used. Secondary antibodies were Rhodamine Red-X (016-290-084; Jackson ImmunoResearch), Alexa 647 (A31571; Life technologies), Alexa 488 (A-11008; Life Technologies) and 1.4 nm nanogold-conjugated anti-rabbit antibody (Nanoprobes, Stony Brook, NY).
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2

Antibodies for Cellular Organelle Analysis

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Antibodies against FAM134C (A304-684A; Bethyl Laboratories, Montgomery, TX, for Western blotting; and HPA16492, Atlas Antibodies, Sweden; for immuno-EM), RTN4B (AB-163; Kinasource Ltd, Dundee, UK), LC3 (L7543; Sigma-Aldrich, Germany), GM130 (610823; BD Biosciences, Franklin Lakes, NJ), ERGIC-53 (ALX-804-602; Enzo Life Sciences, Farmingdale, NY), HA (MMS-101R-50; Covance, Princeton, NJ), FLAG (F7425; Sigma-Aldrich), calreticulin (2679S; Cell Signaling Technologies), green fluorescent protein (GFP) (ab290; abcam, Cambridge, UK), and glyseraldehyde-3-phosphate dehydrogenase (GAPDH;MAB374; Merck Millipore, Billerica, MA) were used as primary antibodies. When indicated, rabbit anti-sheep bridging antibody (313-001-003; Jackson ImmunoResearch Labs, West Grove, PA) was used. Secondary antibodies were conjugated with Rhodamine Red-X (016-290-084; Jackson ImmunoResearch), Alexa 647 (A31571; Life Technologies, Thermo Fisher Scientific) and Alexa 488 (A-11008; Life Technologies). Nanogold-conjugated anti-rabbit secondary antibody (1.4 nm) was used in immuno-EM (Nanoprobes, Stony Brook, NY).
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