This study was approved by the Ethics Committee of Tianjin Hospital. Written informed consent was also obtained from each patient who participated in this research. A total of 45 paired cervical cancer tissues and corresponding adjacent normal tissues were obtained from cervical cancer patients who had undergone surgery between October 2014 and August 2016 in the Department of Obstetrics and Gynecology, Tianjin Hospital. None of the patients received chemotherapy, radiotherapy, or other treatments before surgery. Tissues were immediately frozen in liquid nitrogen and stored at −80°C until further use.
Four human cervical cancer cell lines (HeLa, SiHa, Ca-Ski, and C-33A) were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, P.R. China). Ect1/E6E7, a human normal cervical epithelial cell line, was acquired from the American Type Culture Collection (ATCC; Manassas, VA, USA). All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 mg/ml streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). All cell lines were grown in a humidified atmosphere at 37°C with 5% CO2.
Four human cervical cancer cell lines (HeLa, SiHa, Ca-Ski, and C-33A) were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, P.R. China). Ect1/E6E7, a human normal cervical epithelial cell line, was acquired from the American Type Culture Collection (ATCC; Manassas, VA, USA). All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 mg/ml streptomycin (all from Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). All cell lines were grown in a humidified atmosphere at 37°C with 5% CO2.