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Foundation b fetal bovine serum

Manufactured by Gemini Bio

Foundation B fetal bovine serum is a high-quality cell culture supplement derived from the blood of bovine fetuses. It provides essential nutrients, growth factors, and other components to support the growth and proliferation of a wide range of cell types in vitro.

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2 protocols using foundation b fetal bovine serum

1

Indocyanine Green Cytotoxicity in Neuroblastoma

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KELLY neuroblastoma cells were cultured in Corning RPMI 1640 (Millipore Sigma, Burlington, MA) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Fisher Scientific), 5 mL reconstituted oxaloacetate-pyruvate-insulin (Millipore Sigma), 10% Foundation B fetal bovine serum (Gemini Bioproducts, West Sacramento, CA), and 2 mM L-glutamine (Fisher Scientific). KELLY cells were incubated 24 hours at 37°C in 5% CO2 in humidity to allow surface adhesion before ICG administration. IR-125 laser grade indocyanine green (ICG) was purchased from Fisher Scientific (Waltham, MA). Bovine serum albumin (BSA) was purchased from Millipore Sigma (St. Louis, MO).
For proliferation and viability tests, cells were seeded in a 96-well plate at a density of 12,500 cells/cm2, and were incubated for 24 hours with aqueous ICG, photobleached ICG, or no ICG (control). 20 μL of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS; Promega Corporation, Madison, WI) was added to each well and incubated for 3 hours for each data point. MTS in complete RPMI absent of cells served as a blank for each timepoint. Proliferation data was normalized to the timepoint before treatment. Viability was determined by comparing MTS absorbance of treated cells by normalizing the absorbance of untreated cells tested simultaneously.
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2

Purification and Expression of VWF Fragments

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Ristocetin was purchased from MP Biomedicals. Most recombinant VWF fragments and type 2B mutants were expressed from baby hamster kidney cells as described19 (link),27 (link). Plasma-derived VWF was reconstituted in phosphate-buffered saline (PBS) from outdated lyophilized Humate-P (CSL-Behring). Monoclonal antibodies 6G1, CR1, and 11A8 have been described19 (link),47 (link). ARC117255 (link) was synthesized by Integrated DNA Technologies. Mammalian cells were maintained in DMEM/F12 culture media (ThermoFisher), with 10% Foundation-B fetal bovine serum (Gemini Bio-Products, Sacramento, CA), and 1% penicillin/streptomycin (ThermoFisher). Bovine serum albumin, fraction V was purchased from Fisher Scientific. Tobacco etch virus (TEV) protease was produced with plasmid pD2087 in BL21pRIL cells and purified as described79 (link). The Human GPIb-IX complex was purified from outdated and deidentified leuko-reduced apheresis-derived platelets as described80 (link).
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