For proliferation and viability tests, cells were seeded in a 96-well plate at a density of 12,500 cells/cm2, and were incubated for 24 hours with aqueous ICG, photobleached ICG, or no ICG (control). 20 μL of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS; Promega Corporation, Madison, WI) was added to each well and incubated for 3 hours for each data point. MTS in complete RPMI absent of cells served as a blank for each timepoint. Proliferation data was normalized to the timepoint before treatment. Viability was determined by comparing MTS absorbance of treated cells by normalizing the absorbance of untreated cells tested simultaneously.
Foundation b fetal bovine serum
Foundation B fetal bovine serum is a high-quality cell culture supplement derived from the blood of bovine fetuses. It provides essential nutrients, growth factors, and other components to support the growth and proliferation of a wide range of cell types in vitro.
Lab products found in correlation
2 protocols using foundation b fetal bovine serum
Indocyanine Green Cytotoxicity in Neuroblastoma
Purification and Expression of VWF Fragments
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