Rats were allowed ten days to recover from cannula surgery, and handled on days ten through fourteen following surgery. On day fifteen following surgery, animals were lightly restrained in a towel in order to remove the dust cap and dummy cannula and insert injector cannula. CART 55–102 (Phoenix Peptides, USA) was dissolved in isotonic sterile 0.9% saline at a concentration of 5μg/μl. Previous work demonstrated this dose was within a range that would not cause alterations in motor behavior (17 (link), 34 (link)). Bilateral intra-accumbal injections of 0.3μl were administered by two-2ul Hamilton microsyringes (Hamilton Co., Reno, NV) controlled by a microinfusion pump (Harvard Apparatus, Holliston, MA) for a final delivery of 1.5ug per side. Microsyringes were connected to 33-gauge injector cannulae (PlasticsOne, VA) by polyethylene tubing. Displacement of an air bubble in the polyethylene tubing was used to monitor injection. Injections occurred over 90 seconds, with 30 seconds allowed after the injection to permit spread of the drug or vehicle. Immediately following the injection, dummy cannula and dust-caps were replaced and the animal was returned to its home-cage for 5 minutes prior to being introduced to the open field arena for assessment of novelty suppressed feeding. Treatment groups were HCR-Veh (n=6), LCR-Veh (n=6), HCR-CART (n=6), and LCR-CART (n=6).
Microinfusion pump
Manufactured by Harvard Apparatus
Sourced in United States, France
The Microinfusion Pump is a precision laboratory instrument designed for the controlled delivery of small volumes of fluids. It features programmable flow rates and supports a range of syringe sizes to accommodate diverse experimental requirements. The pump operates on the principle of a stepper motor and lead screw mechanism to ensure accurate and consistent fluid delivery.
Automatically generated - may contain errors
Lab products found in correlation
Hamilton syringe, by Hamilton Company (5 mentions)
Stereotaxic frame, by Kopf Instruments (5 mentions)
Stereotactic frame, by Kopf Instruments (4 mentions)
Clostridial collagenase 7 s, by Merck Group (3 mentions)
Microliter 701, by Hamilton Company (2 mentions)
Muscimol, by Merck Group (2 mentions)
Clostridial collagenase, by Merck Group (2 mentions)
Angiotensin 2, by Merck Group (2 mentions)
Hamilton syringe, by Harvard Apparatus (2 mentions)
Microliter no 701, by Hamilton Company (2 mentions)
Non puncture ear bars, by RWD Life Science (2 mentions)
Nas 181, by Bio-Techne (2 mentions)
Naltrexone, by Bio-Techne (1 mentions)
β fna, by Merck Group (1 mentions)
Nor bni, by Bio-Techne (1 mentions)
4 hydroxytamoxifen 4 oht, by Merck Group (1 mentions)
Microsyringe, by Hamilton Company (1 mentions)
Sirna dilution buffer, by Santa Cruz Biotechnology (1 mentions)
Pe 10, by BD (1 mentions)
Model 500, by Kopf Instruments (1 mentions)
68 protocols using microinfusion pump
1
Bilateral Intra-Accumbal CART Infusion in Rats
2
Pharmacological Modulation of Opioid Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ketamine (10–30 mg/kg, MWI Veterinary Supply Co.), Naltrexone (5 mg/kg, Tocris, 0677), beta-funaltrexamine (β-FNA, 15 mg/kg, Sigma Aldrich, O003), norbinaltorphimine (norBNI, 10 mg/kg, Tocris, 0347), and cocaine (10 mg/kg, Sigma Aldrich, C5776) were all dissolved in 0.9% normal saline. 4-hydroxytamoxifen (4OHT, 50 mg/kg, Sigma Aldrich, T176) was dissolved in warm ethanol (50 μl / mg 4OHT) and mixed into sunflower oil (80 μl / mg 4OHT) and castor oil (20 μl / mg 4OHT), followed by vacuum centrifugation to evaporate out the ethanol. Drugs were administered intraperitoneally (i.p.) at a volume of 10 mL/kg. For all experiments involving drug injection, matched control groups always received the same total number of drug or SAL injections.
For local inhibition of MORs in the CeA, mice were bilaterally microinjected with CTAP (300 ng in 500 nL in saline, Sigma Aldrich, C6352) 5 min before behavioral testing. CTAP was infused through an injector cannula coupled to a 5 μL Hamilton syringe using a microinfusion pump (Harvard Apparatus) at a continuous rate of 150 nL min−1 to a total volume of 0.5 μL per hemisphere. Injector cannulas were removed 1 min after infusions were complete.
For local inhibition of MORs in the CeA, mice were bilaterally microinjected with CTAP (300 ng in 500 nL in saline, Sigma Aldrich, C6352) 5 min before behavioral testing. CTAP was infused through an injector cannula coupled to a 5 μL Hamilton syringe using a microinfusion pump (Harvard Apparatus) at a continuous rate of 150 nL min−1 to a total volume of 0.5 μL per hemisphere. Injector cannulas were removed 1 min after infusions were complete.
3
Hemorrhagic Stroke Induction in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three C57Bl/6 mice (postnatal week 8) were used to surgically induce hemorrhagic stroke according to the previous protocol with a few modifications.42 (link) In brief, the mice were anesthetized as described before and followed with an intravenous injection of 200 μL of L1013 NPs (3.7 mg mL‒1). We carefully removed the temporal scalp to expose the skull, and made a small cranial burr hole. Then, 10 μL of autologous blood collected from tail blood vessels was injected into the right subcortex (bregma coordinates: 2 mm posterior and 3 mm lateral to the midline, and 1 mm in depth) at a rate of 2 μL min‒1 using a microinfusion pump (Harvard Apparatus, Holliston, MA) under stereotactic guidance. Afterwards, the mice were subjected to NIR-II imaging immediately. After the NIR-II imaging, the mice were sacrificed and the whole brain was removed from the skull. The brain was post-fixed by formalin and dehydrated with 30% sucrose solution for 2 days at 4°C. Then the brain was embedded in optimal cutting temperature compound (O.C.T.; Sakura Finetek USA, Inc., Torrance, CA) and cut into 30-µm-thick sections. Finally, the brain slices were observed under microscope.
4
Intracerebroventricular Drug Delivery in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Intracerebroventricular drug injection was performed as our previous study43 (link). Briefly, rats were anesthetized with a pentobarbital (50 mg/kg) intraperitoneal injection and then fixed onto a stereotaxic head apparatus. The 26-gauge needle of a 10-μL Hamilton syringe (Microliter 701; Hamilton Company, Reno, NV) was inserted into the left lateral ventricle through a cranial burr hole at the following coordinates relative to bregma: 1.5 mm posterior; 1.0 mm lateral; 3.2 mm below the horizontal plane of bregma. Then drugs were infused by a microinfusion pump (Harvard Apparatus, Holliston, MA) at a rate of 0.5 μl/min. The needle was left in place for an additional 15 minutes after the infusion finishing, and then the incision was closed with sutures.
5
VTA Infusion of DK-AH 269 in Stress-Susceptible Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two groups of stress-susceptible mice received a single dose VTA infusion of DK-AH 269 (0.6 μg, also called cilobradine, Sigma-Aldrich, CAS #: 186097-54-1) or PBS as control. Specifically, 24 h after their last defeat stress (or control condition), mice were placed under a combination of ketamine (100 mg/kg) and xylazine (10 mg/kg) anesthesia for bilateral implantation of 26 gauge guide cannulae fitted with obturators as described previously (Friedman et al., 2016 ). The cannulae were secured to the skull after being positioned 1 mm directly above the VTA (AP, −3.2; ML, 0.4; DV, −3.6 mm). After 5 days of postoperative recovery, simultaneous bilateral microinjections of DK-AH 269, or PBS (Vehicle) were delivered through an injector cannula in a total volume 0.4 μl/side at a continuous rate of 0.1 μl/min, under the control of a micro-infusion pump (Harvard Apparatus). The concentration of DK-AH 269 was selected based on earlier in vivo studies (Chevaleyre and Castillo, 2002 (link)). Injector cannulae were removed 5 min after the stopping of each infusion, and mice remained undisturbed in their home cages for an additional 1 h before testing for social interaction behavior.
6
Intracerebroventricular Injection in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the i.c.v. injection, the mice were anesthetized using 5% isoflurane in 30% O 2 and 70% N 2 O and placed into the stereotactic frame (David Kopf Instruments, CA, USA). The anesthetic was reduced to 2% for maintenance and the body temperature was maintained at 36.5 ± 0.5 °C using a homoeothermic blanket (PanLab, Barcelona, Spain). The head of the anesthetized mouse was fixed using the stereotactic frame. After making an incision on the shaved and disinfected scalp, a blunt needle (33G) of a 5-µl Hamilton syringe (Hamilton, NV, USA) was aligned to the following coordinates: + 0.3 mm anterior/posterior (A/P), + 1.0 medial/lateral (M/L) from bregma. Using a dental burr head (Meissinger, Duesseldorf, Germany), a small hole was drilled into the skull above the injection site without injuring the meninges. Subsequently, the needle was inserted into the brain tissue (-2.0 mm dorsal/ventral (D/V) below the dural surface). A single dose of either VEGF-C or Ctrl diluted in phosphate-buffered saline (PBS, final volume of 4 µl) was infused into the left ventricle with a microinfusion pump (Harvard Apparatus, MA, USA) at a speed of 0.5 µl/min for 8 min. After injection, the needle was kept at the injection site for 4 min before slow withdrawal.
7
Microinjection of Compounds into Mouse mPFC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each mouse was anesthetized with pentobarbital (50 mg/kg, i.p.) and fixed to a brain stereotaxic apparatus (Narishige Instruments). For the injection into the mPFC, the brains were implanted with guide cannulas (Eicom) bilaterally, so that the tips were positioned near the mPFC (anteroposterior, 2.0 mm from bregma; lateral, ±1.4 mm; ventral, -2.3 mm; angle, 20°). The cannulas were held in place with dental cement. A dummy cannula was inserted into the guide cannula to prevent clogging. Microinjection of the compounds was performed on day 2 or 3 after the surgery. Before the microinjections, the dummy cannulas were removed from the guide cannula, and a 28-gauge injection cannula, extending 0.5 mm from the tip of the guide cannula, was inserted. The injection cannula was connected via a Teflon tubing to a micro syringe (Hamilton Co) driven by a micro infusion pump (Harvard Apparatus, Inc). Injections of the compounds were performed for 2 minutes at the rate of 0.05 μL/min. The injection cannulas were left in position for an additional 2 minutes before being withdrawn. After the behavioral test, Evans blue was infused, followed by preparation of coronal sections to confirm the locations of the cannula tips. The locations of the cannula tips are shown in supplementary Figures 1 to 12 .
8
Rat Model of Intracerebral Hemorrhage
9
Intra-Cerebral Delivery of TAT-NET Peptides
10
GABAA Receptor Agonist Microinjection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Infusions of the GABAA receptor agonist muscimol (1 mg/mL; Sigma-Aldrich, St. Louis, MO, United States) or vehicle (0.9% sterile physiological saline) were given in a 1 μL volume at a rate of 0.25 μL/min. Internal stainless steel microinjectors (28 gauge, Plastics One) protruding 1 mm below implanted guides were connected via polyethylene tubing (PE50, Plastics One) to 10 μL syringes (Hamilton, Franklin, MA, United States) mounted in a microinfusion pump (Harvard Apparatus, Holliston, MA, United States). Tubing was backfilled with autoclaved water. A 1-μL bubble was aspirated to create a barrier between backfill and infusate, and permit confirmation by visual inspection that the correct volume of infusate had been delivered. Microinjectors were left in place for 2 min after the infusion to allow dispersion of the drug. Rats were then returned to the home cage for 30 min prior to beginning behavioral testing.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!