All-trans retinal (ATR) plates were prepared (100 mM stock in ethanol, final working 2.7 mM in OP50). Plates were stored at 4°C under dark conditions and used within 1 week. Animals were grown on +ATR OP50 plates in dark and L4 animals were transferred to a fresh +ATR plate prior to the day of experiment. Experiments were performed using 1-day adults. For ChR2 photostimulation, experiments were conducted using a fluorescent dissecting microscope (Zeiss stereo Discovery.V12) equipped with a GFP filter set. Behavior was recorded for a 1-min period prior to photostimulation and during a subsequent 1 min period during photostimulation. Data are expressed as % change in reorientations across these time intervals. Chrimson photostimulation (26 mW/cm2) experiments were conducted using a 625 nm 50 W LED (Mightex Systems). Animals were video recorded for 1 min in the absence of light stimulation (prestimulus) and subsequently for 1 min with light stimulation. Control experiments (−ATR) were performed in the same manner.
625 nm 50 w led
Manufactured by Mightex
The 625 nm 50 W LED is a high-power light-emitting diode that produces light at a wavelength of 625 nanometers. It has a power output of 50 watts.
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2 protocols using 625 nm 50 w led
1
Optogenetic Manipulation of C. elegans Behavior
2
Optogenetic Manipulation of C. elegans Behavior
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All-Trans Retinal (ATR) plates were prepared (100 mM stock in ethanol, final working 2.7 mM in OP50). Plates were stored at 4°C under dark conditions and used within one week. Animals were grown on +ATR OP50 plates in dark and L4 animals were transferred to a fresh +ATR plate prior to the day of experiment. Experiments were performed using one-day adults. For ChR2 photostimulation, experiments were conducted using a fluorescent dissecting microscope (Zeiss stereo Discovery.V12) equipped with a GFP filter set. Behavior was recorded for a 1minute period prior to photostimulation and during a subsequent 1-minute period during photostimulation. Data are expressed as % change in reorientations across these time intervals.
Chrimson photostimulation (26 mW/cm 2 ) experiments were conducted using a 625 nm 50 W LED (Mightex Systems). Animals were video recorded for 1 minute in the absence of light stimulation (prestimulus) and subsequently for 1 minute with light stimulation. Control experiments (-ATR) were performed in the same manner.
Chrimson photostimulation (26 mW/cm 2 ) experiments were conducted using a 625 nm 50 W LED (Mightex Systems). Animals were video recorded for 1 minute in the absence of light stimulation (prestimulus) and subsequently for 1 minute with light stimulation. Control experiments (-ATR) were performed in the same manner.
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