Anti mouse igg tritc

Manufactured by Jackson ImmunoResearch

Sourced in Cameroon

Anti-mouse IgG-TRITC is a secondary antibody conjugated with the fluorescent dye TRITC (Tetramethylrhodamine). It is designed to detect and visualize primary antibodies raised against mouse immunoglobulin G (IgG) in various immunoassay techniques.

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Lab products found in correlation

Rhodamine phalloidin, by Thermo Fisher Scientific (3 mentions) A1 confocal system, by Nikon (2 mentions) Anti mouse ly6g alexafluor647, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Anti rabbit igg fitc, by Vector Laboratories (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Prolong gold anti fade reagent containing dapi, by Thermo Fisher Scientific (1 mentions) Anti mouse igg alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Elements software, by Nikon (1 mentions) Fluorescence mounting medium, by Agilent Technologies (1 mentions) Ds qi2, by Nikon (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Nis elements ar software, by Nikon (1 mentions)

3 protocols using anti mouse igg tritc

Immunofluorescent Labeling of Lung Cells

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The following antibodies were used for immunofluorescent labeling: anti-claudin-3 (Life Technologies), anti-rabbit IgG-FITC (Vector Laboratories, Burlingame, CA), anti-occludin-AlexaFluor⁴⁸⁸ (Life Technologies), anti-histone H2A/H4 (BWA3), anti-mouse IgG-TRITC (Jackson Immunoresearch), anti-mouse Ly6G-AlexaFluor⁶⁴⁷ (eBioscience, San Diego, CA), anti-mouse CD11c-AlexaFluor⁴⁸⁸ (eBioscience), anti-mouse surfactant A (Millipore), anti-rabbit IgG-AlexaFluor⁶⁴⁷ (Jackson Immunoresearch). Slides were mounted with ProLong Gold anti-fade reagent containing DAPI (Life Technologies). Digital monochromatic images were acquired on a Nikon A-1 confocal system with Nikon Elements software and pseudocolored. For quantitative analysis of histone-associated cells, greater than 200 histone-associated cells were analyzed per lung (n=3 mice) for cell type-specific labeling (neutrophil, macrophage, or type II alveolar epithelial cells). The percentage of histone-associated cells that were also labeled for the cell type-specific marker is displayed.

Grailer J.J., Canning B.A., Kalbitz M., Haggadone M.D., Dhond R.M., Andjelkovic A.V., Zetoune F.S, & Ward P.A. (2014). Critical role for the NLRP3 inflammasome during acute lung injury. Journal of immunology (Baltimore, Md. : 1950), 192(12), 5974-5983.

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Confocal Imaging of Activated Immune Cells

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For confocal imaging of cells, PMNs and macrophages were plated on sterile 22 mm coverslips in 6-well plates for 1–2 hr to let cells adhere as best as possible. Then the activator (C5a or PMA) was added and incubated for 90 minutes at 37°C. Immunofluorescence staining followed. For studying uptake of histones, cells were incubated with FITC-conjugated histones or AF488-H1 up to 30 min at 37°C.
The following antibodies were used for immunofluorescent labeling: anti-mouse IgG-TRITC (Jackson Immunoresearch, West Grove, PA), anti-mouse IgG-AlexaFluor488 (Jackson Immunoresearch, West Grove, PA), and anti-mouse Ly6G-AlexaFluor647 (eBioscience, San Diego, CA). Slides were mounted with ProLong Gold anti-fade reagent containing DAPI (Life Technologies Grand Island, NY). Digital monochromatic images were acquired on a Nikon A-1 confocal system with Nikon Elements software.

Fattahi F., Grailer J.J., Jajou L., Zetoune F.S., Andjelkovic A.V, & Ward P.A. (2015). Organ Distribution of Histones after Intravenous Infusion of FITC-Histones or after Sepsis. Immunologic research, 61(3), 177-186.

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Immunofluorescent Visualization of αIIbβ3 Integrin

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CHO/αIIbβ3 cells were plated onto cover glasses and transfected with various DNA constructs. One day after transfection, the cells were fixed using a 3.7% formaldehyde solution in PBS, permeabilized with PBS containing 0.1% Triton X-100, and subsequently blocked using PBS supplemented with 5% goat serum, 0.03% gelatin, and 0.1% Triton X-100 for 30 min. Cells were then stained with anti-integrin αIIbβ3 (D57) followed by anti-mouse IgG-TRITC (Jackson Laboratory), each for 30 min. Rhodamine phalloidin (Thermo Fisher) was employed for actin staining when needed. The cover glasses with the stained cells were mounted onto slide glasses using Fluorescence Mounting Medium (Dako) containing Hoechst 33342 (Invitrogen) for nucleus visualization. Analysis was conducted using a fluorescence microscope (Ti-E, Nikon) equipped with a 100× (1.4 NA) plan-apochromat objective lens. Fluorescence images were captured using a charge-coupled device camera (DS-Qi2, Nikon) and deconvoluted using NIS-Elements AR software (Nikon).

Lee J., Lee M., Kim J., Cho E.G, & Kim C. (2024). Producing highly effective extracellular vesicles using IBAR and talin F3 domain fusion. Animal Cells and Systems, 28(1), 283-293.

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