Anti rhogdi sc 360

Manufactured by Santa Cruz Biotechnology

Sourced in United States, United Kingdom

Anti-RhoGDI (sc-360) is a rabbit polyclonal antibody that recognizes the RhoGDI protein. RhoGDI is a regulator of the Rho family of small GTPases, which are involved in various cellular processes.

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RhoGDI (6 citations) Anti-RhoGDI (5 citations) Sc-360 (2 citations)

3 protocols using anti rhogdi sc 360

Cell Culture Reagents and Antibodies

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Cell culture media RPMI1640, DMEM, TC100 and supplements (Newborn calf serum, penicillin/streptomycin, glutamine, trypsin-EDTA) were from Gibco Life Technologies (Paisley, UK). NCTC 109 medium and chloroquine were from Sigma (Steinheim, Germany). Foetal bovine serum (FBS) was from Lonza Inc. (Walkersville, MD, USA). Lipofectamine 2000 and Lysotracker Red (LTR) were from Life Technologies (Carlsbad, CA, USA). U18666A was from Merck4Biosciences (Madrid, Spain). Anti-GFP was from Roche Diagnostics GmbH (Mannheim, Germany). Anti-Hsp90 (sc-7947), anti-RhoGDI (sc-360), and anti-vimentin (sc-6260) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse Igs were from Dako (Copenhagen, Denmark). Anti-giantin was from Covance (Princeton, NJ, USA).

Oeste C.L., Pinar M., Schink K.O., Martínez-Turrión J., Stenmark H., Peñalva M.A, & Pérez-Sala D. (2014). An Isoprenylation and Palmitoylation Motif Promotes Intraluminal Vesicle Delivery of Proteins in Cells from Distant Species. PLoS ONE, 9(9), e107190.

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Monoclonal Antibody Sourcing Protocol

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Monoclonal antibodies were sourced as follows: anti-YAP (sc-101199) and anti-TEF-1 (sc-376113) from Santa Cruz Biotechnology; anti-CAV1 XP (#3267) and anti-YWHAH (14-3-3 η (D23B7); #5521) from Cell Signaling (Danvers, Massachusetts, United States); anti-Flag M2 (F-3167) from Sigma-Aldrich; and anti-glyceraldehyde-3-phosphate dehydrogenase (MAB374) and anti-cortactin (p80/85, clone 4F11) from Millipore (Burlington, Massachusetts, United States). Polyclonal antibodies to phospho-YAP (Ser127) (#9411), LATS1 (#9153), and LATS2 (#13646) were from Cell Signaling; anti-Histone H3 (ab1791) was from Abcam (Cambridge, United Kingdom); and anti-RHO GDI (sc-360) was from Santa Cruz Biotechnology. For immunohistochemistry, we used anti-YAP (D8H1X) XP from Cell Signaling (#14074) and αSMA from Thermo Fisher Scientific (RB-9010-P0).

Moreno-Vicente R., Pavón D.M., Martín-Padura I., Català-Montoro M., Díez-Sánchez A., Quílez-Álvarez A., López J.A., Sánchez-Álvarez M., Vázquez J., Strippoli R, & del Pozo M.A. (2018). Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP. Cell Reports, 25(6), 1622-1635.e6.

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Quantifying EGF-mediated cellular signaling

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EGF (10ng/ml) was purchased from PeproTech. Anti-UBTD1 (HPA034825), sodium chloride, DAPI, Gefitinib (SML1657) and 2,5-dihydroxybenzoic acid (DHB) were purchased from Sigma-Aldrich. Anti-HSP90 (sc-13119), anti-RhoGDI (sc-360) and anti-Ubiquitin (sc-8017) were purchased from Santa Cruz Biotechnology. Anti-pSTAT3 (clone D3A7, 9145), anti-STAT3 (clone 124H6, 9139), anti-p-EGF receptor (2234) and anti-EGF receptor (2232) were purchased from Cell Signaling. Anti-EEA1 (610457) and anti-CD107a/LAMP1 (555798) were purchased from BD Biosciences. Anti-SQSTM1/P62 (GTX100685) was purchased from Genetex. Anti-calreticulin-ER Marker (ab2907) was purchased from Abcam. DQ™ Red BSA (D12051) was purchased from Invitrogen. Ganglioside GM3 (860058) was purchased from Avanti Polar Lipids, HRP-conjugated donkey anti-mouse IgG (715-035-150) and HRP-conjugated anti-mouse IgG (711-035-152) were purchased from Jackson ImmunoResearch Laboratories. Epidermal Growth Factor, biotinylated, complexed to Alexa Fluor 647 Streptavidin (Alexa Fluor 647 EGF complex) was purchased from Molecular Probes, Invitrogen (E35351).

Torrino S., Tiroille V., Dolfi B., Dufies M., Hinault C., Bonesso L., Dagnino S., Uhler J.P., Irondelle M., Gay A., Fleuriot L., Debayle D., Lacas‐Gervais S., Cormont M., Bertero T., Bost F., Gilleron J., & Clavel S. (2020). UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling.

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