To assess the lactate dehydrogenase (LDH) and creatine kinase (CK) activities, blood samples were collected, centrifuged and stored at −20°C until analysis. The samples were analyzed using standard techniques using an LDH Assay kit and CK Assay kit in accordance with the manufacturer’s instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Values were expressed as international units (IU) per liter.
Ck assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The CK assay kit is a laboratory product designed to measure the activity of the enzyme creatine kinase (CK) in biological samples. CK is an important enzyme involved in the production and transfer of energy within cells. The kit provides the necessary reagents and protocols to quantify CK levels, which can be used for various clinical and research applications.
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4 protocols using ck assay kit
1
Quantification of LDH and CK Levels
2
Whole Blood Analysis and Biochemical Markers
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Whole blood samples (20 μL) were hemodiluted with diluent NK (2 mL; Nihon Kohden Co., 8-522) and then measured using the MEK-6318K Automatic Blood Cell Analyzer (Nihon Kohden Co.). The activity/levels of serum creatine kinase (CK), creatinine (Cr), and alanine aminotransferase (ALT) were evaluated with the Beckman Au2700 Automatic Biochemical Analyzer (Beckman Coulter, Inc.) using CK assay kit (Nanjing Jiancheng Biotech. A032), Cr assay kit (Nanjing Jiancheng Biotech. C011-1), ALT assay kit (BioSino Bio-technology. 142331) The serum concentration of tumor necrosis factor alpha (TNF-α; 20140403AB5), lipopolysaccharide (LPS), interleukin 6 (IL-6; 20140421BG1), interleukin 10 (IL-10; 20140423MC1), CK-myocardial band (CK-MB; 20140421ND5), and cardiac troponin I (cTnI; 20140421MG3) was detected via the ELISA kits from Shanghai Zhuokang Biological Technology.
3
Serum LDH and CK Biomarkers for Tissue Injury
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As a biomarker for tissue injury, the activities of serum LDH and CK were measured by a microplate reader (Bio-rad 680, Hercules, CA, USA) according to the specifications of the LDH assay kit and CK assay kit (Jiancheng, Nanjing, China).
4
Cardiac Biomarkers and Antioxidant Evaluation
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After successful I/R modelling and treatment with different doses of modified THSWD for 24 h, rat blood was collected to obtain serum. The levels of serum creatine kinase (CK) and lactate dehydrogenase (LDH) and the activity of superoxide dismutase (SOD) were determined using the CK assay kit, LDH assay kit, and SOD assay kit (Nanjing Jiancheng Bioengineering Institute), respectively, according to the manufacturer’s instructions.
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