Taq polymerase chain reaction (PCR) reagents were acquired from Takara (Kyoto, Japan), and the pET21b (+) plasmid was purchased from Novagen (Birmingham, United Kingdom). Peptides were synthesized by PepTron Inc. (Daejeon, South Korea). P (S/V-COOH) Europium nanoparticle (Eu NP) beads (0.1 µm) were purchased from Bangs Laboratories Inc. (Fishers, IN, USA). N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide sodium salt (NHS), and N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) were acquired from Thermo Scientific (Waltham, MA, USA). Recombinant antigen and mAbs (1A5 and 1G8) were developed and are described in Supplementary Material (Figure S1 ).
N hydroxysulfosuccinimide sodium salt sulfo nhs
Manufactured by Thermo Fisher Scientific
Sourced in United States
N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) is a water-soluble, amine-reactive crosslinking reagent. It is used to modify proteins and other biomolecules with primary amines.
Automatically generated - may contain errors
Lab products found in correlation
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc, by Thermo Fisher Scientific (6 mentions)
Europium nanoparticles, by Bangs Laboratories (3 mentions)
Aliphatic amine latex beads, by Thermo Fisher Scientific (2 mentions)
Goat anti mouse igg h l fict ab6758, by Abcam (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
N hydroxysulfosuccinimide sodium salt nhs, by Thermo Fisher Scientific (1 mentions)
Polyclonal goat anti mouse igg, by Merck Group (1 mentions)
Rabbit anti mouse igg h l, by Abcam (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Thermo Fisher Scientific (1 mentions)
Iodogen, by Thermo Fisher Scientific (1 mentions)
2 n morpholino ethanesulfonic acid, by Merck Group (1 mentions)
Vivaspin 500, by Sartorius (1 mentions)
Herceptin, by Roche (1 mentions)
Disodium hydrogen phosphate, by Merck Group (1 mentions)
Pbs ph 7, by Thermo Fisher Scientific (1 mentions)
Hydroquinone, by Merck Group (1 mentions)
N ethyl n 3, by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Tween 20, by Merck Group (1 mentions)
Quantipro bca assay kit, by Merck Group (1 mentions)
9 protocols using n hydroxysulfosuccinimide sodium salt sulfo nhs
1
Immunoassay Reagents and Nanoparticle Conjugation
2
Europium Nanoparticle Antibody Conjugation
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Europium nanoparticles (200-nm diameter) were purchased from Bangs Laboratories Inc. (Fishers, IN, USA). Aliphatic amine latex beads (100-nm diameter) were purchased from Life Technology (Carlsbad, CA, USA). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-RSV Fusion and anti-RSV nucleoprotein (NP) were purchased from Abcam (Cambridge, UK). Rabbit anti-mouse IgG H&L (horseradish peroxidase (HRP)) and goat anti-mouse IgG H&L (FICT) ab6758 were obtained from Abcam.
3
Europium Nanoparticle-Based Influenza Detection
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Europium nanoparticles were purchased from Bangs Laboratories Inc. (Fishers, IN, USA). Aliphatic amine latex Beads (100 nm diameter) were purchased from Life Technology (Carlsbad, CA, USA). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) were purchased from Thermo Scientific (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) without further purification. Recombinant hemagglutinin 1 (rHA1) of H7N9 (A/Anhui/1/2013) and rHA1 of H5N1 (A/Vietnam/1/2003) were purchased from Sino Biological Inc. (Beijing, China). Anti-influenza A nucleoprotein (NP) (Clone 3G6) was provided by Professor Ho-Joon Shin, Ajou University, Suwon, Republic of Korea. Polyclonal goat anti-mouse IgG was purchased from Sigma-Aldrich.
4
Rapid Influenza A Detection Assay
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Rabbit anti-mouse IgG H&L (horseradish peroxidase (HRP)) and goat anti-mouse IgG H&L (FICT) ab6758 were bought from Abcam (Cambridge, UK). Europium nanoparticles (200 nm diameter) were purchased from Bangs Laboratories Inc. (Fishers, IN, USA). Colloidal gold (40 nm diameter) was obtained from Bore Da Biotech Co., (Gyeonggi, Republic of Korea). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) were acquired from Thermo Scientific (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used without further purification. Anti-influenza A nucleoprotein (NP) (Clone 3D3) was provided by Professor Ho-Joon Shin, Ajou University, Suwon, Republic of Korea.
5
Europium Nanoparticle-Based Detection of Influenza A
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Monoclonal antibodies (mAb), including anti-influenza A nucleoprotein (NP) (7307 and 7304), were purchased from Medix Biochemica (Espoo, Finland). Europium nanoparticles (Eu NP) (200 nm) were acquired from Bangs Laboratories Inc. (Fishers, IN, USA). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) were obtained from Thermo Scientific (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Influenza A/H1N1/2009/CA was provided by Korea Centers for Disease Control and Prevention. It was grown and titrated in eggs as previously described18 (link). Influenza A/H3N2 isolated from human in 2014 was donated from Korean National Research Resource Center (Seoul, Korea) (registration number: KBPV-VR-85).
6
Radiolabeling of Trastuzumab Monoclonal Antibody
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The used chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA without further purification: iron(III) chloride hexahydrate (ACS reagent, 97%), 3-phosphonopropionic acid (CEPA, 94%) and human serum (stored at −22 °C), 2-(N-morpholino)ethanesulfonic acid (MES, ≥99%). Iodogen (1,3,4,6-tetrachloro-3α,6α-diphenyl-glycoluril),N-(3-dimethylaminopropyl)-N′-ethylcarbo-diimide hydrochloride (EDC, ≥99%) and N-hydroxysulfosuccinimide sodium salt (sulfo-NHS, ≥98%) were obtained from Thermo Scientific (Rockford, IL, USA). Monoclonal antibody, trastuzumab, was separated from the commercial product (Herceptin®, Roche Pharmaceuticals, Basel, Switzerland) on Vivaspin 500 (Sartorius, Stonehouse, UK) with a 50-kDa cutoff membrane. Aqueous solutions were obtained from ultrapure deionized water prepared using the Milli-Q filtering system (Merck, Darmstadt, Germany).
7
Peptide-Labeled Europium Nanoparticle Assay
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Peptides were synthesized by PepTron Inc. (Daejeon, South Korea). P (S/V-COOH) Europium nanoparticle (Eu NP) beads (0.2 µm) were purchased from Bangs Laboratories Inc. (Fishers, IN, USA). N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) were purchased from Thermo Scientific (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). H5 subtype-specific monoclonal antibodies (clones 3F11 and 1C5) were kindly provided by Dr. Chom-Kyu Chong (GenBody Inc., Cheonan, South Korea).
8
Immunosensor for Cytokine Detection
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Disodium hydrogen phosphate, sodium phosphate dibasic, tris-(hydroxymethyl)-aminomethane (TRIS), N-ethyl-N′-(3-dimethylaminopropyl)-carbodiimide (EDC), bovine serum albumin (BSA), Casein, Tween 20, hydrogen peroxide 30%, Triton X-100, Halt Protease & Phosphatase EDTA-free Inhibitor Cocktail, QuantiPro BCA Assay Kit, and hydroquinone were obtained from Sigma-Aldrich (Munich, Germany). PBS pH 7.2 (Gibco), RPMI-1640 medium, and foetal bovine serum (FBS) were obtained from Thermo Fisher Scientific (Schwerte, Germany). Sodium chloride was obtained from Merck (Darmstadt, Germany). 2-(N-Morpholino)-ethanesulfonic acid (MES) was purchased from Amresco (Solon, OH, USA). N-Hydroxysulfosuccinimide sodium salt (Sulfo-NHS) was obtained from Alfa Aesar (Heysham Lancashire, UK). Immunoreagents were from the human IL-13 DuoSet ELISA Kit obtained from Bio-Techne (Wiesbaden, Germany). For the immunosensor, a streptavidin–horseradish peroxidase (HRP) conjugate (ABIN964537) from antibodies-online was applied (Aachen, Germany).
9
Magnetite Nanoparticles for Biomedical Applications
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All reagents used in the study were used as purchased without further purification. These include iron(iii ) chloride hexahydrate (FeCl3·6H2O, Sigma-Aldrich), ethylene glycol (99.5%, SAMCHUN), sodium acetate anhydrous (NaOAc, Duksan), polyacrylic acid (PAA, MW: 5000, 50% aqueous solution, Acros Organics), N,N′-dicyclohexylcarbodiimide (DCC, Sigma-Aldrich), 4-dimethylaminopyridine (DMAP, Sigma-Aldrich), biotin (Sigma-Aldrich), dimethyl sulfoxide (DMSO, Sigma-Aldrich), fluorescein isothiocyanate (FITC, Sigma-Aldrich), (3-aminopropyl)trimethoxysilane (APTMS, Sigma-Aldrich), tetraethyl orthosilicate (TEOS, TCI), ethyl alcohol (95%, SAMCHUN), ammonia solution (28–30%, SAMCHUN), potassium chloride (99%, SAMCHUN), egg-white avidin (Sigma-Aldrich), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, >98%, Acros Organics), N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS, Alfa Aesar), Arg-Gly-Asp (RGD, ≥97%, Sigma-Aldrich) and photopolymer resin (XYZ Print), Lipofectamine™ 3000 Transfection Reagent (Invitrogen), DMEM (high glucose, Simplybiologics). A549 cells were purchased from American Type Culture Collection (USA) and grown in Dulbecco's modified Eagle Medium supplemented with 10% fetal bovine serum. Blood was obtained after CO2 euthanasia of a mouse, and all animal procedures were approved by the Animal Ethics Committee of Chungnam National University.
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