Cf633 wheat germ agglutinin wga

Manufactured by Biotium

CF633 wheat germ agglutinin (WGA) is a fluorescent labeling reagent. WGA is a lectin that binds to N-acetylglucosamine and sialic acid residues on cell surfaces. The CF633 dye provides red fluorescence for visualization of WGA-labeled samples.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti mouse igg hrp, by Santa Cruz Biotechnology (3 mentions) Biotinylated peanut agglutinin pna, by Vector Laboratories (2 mentions) Extravidin peroxidase, by Merck Group (2 mentions) Human cd227, by BD (2 mentions) Actin, by Santa Cruz Biotechnology (1 mentions) U0126, by Cayman Chemical (1 mentions) Doxycycline, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using cf633 wheat germ agglutinin wga

Glycoprotein Immunofluorescence Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: FITC-human CD227 (Muc1) (559774, BD Biosciences), human CD227 (555925, BD Biosciences) (Muc1), Alexa-488-human podocalyxin (222328, R&D Systems), actin (sc1615, Santa Cruz), goat anti-mouse IgG-HRP (sc-2005, Santa Cruz), and mouse anti-goat IgG-HRP (sc-2354, Santa Cruz). Lectins used were: biotinylated peanut agglutinin (PNA; B-1075, Vector Laboratories), CF568 Arachis hypogaea Lectin PNA (29061, Biotium), CF640R Arachis hypogaea lectin PNA (29063, Biotium), CF633 wheat germ agglutinin (WGA; 29024, Biotium), and FITC concanavalin A (ConA; FL-1001, Vector Laboratories). Biotinylated lectins were detected using ExtrAvidin-Peroxidase (E2886, Sigma). MAPK inhibitor was U-0126 (70970, Cayman Chemical). To induce transactivator cell lines, doxycycline was used (sc-204734, Santa Cruz).

Shurer C.R., Colville M.J., Gupta V.K., Head S.E., Kai F., Lakins J.N, & Paszek M.J. (2017). Genetically Encoded Toolbox for Glycocalyx Engineering: Tunable Control of Cell Adhesion, Survival, and Cancer Cell Behaviors. ACS biomaterials science & engineering, 4(2), 388-399.

+ Open protocol

+ Expand

Cell Surface Glycoprotein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: Human CD227 (555925, BD Biosciences) (Muc1), β-Actin (sc-4778, Santa Cruz), Goat anti-Mouse IgG-HRP (sc-2005, Santa Cruz). Lectins used were: Biotinylated Peanut Agglutinin (PNA; B-1075, Vector Laboratories), CF568 PNA (29061, Biotium), CF640R PNA (29063, Biotium), CF633 Wheat Germ Agglutinin (WGA; 29024, Biotium). Biotinylated lectins were detected using ExtrAvidin-Peroxidase (E2886, Sigma). To induce transactivator cell lines, doxycycline was used (sc-204734, Santa Cruz). For gentamycin selection, G418 was used (10131035, Thermo Fisher).

Shurer C.R., Head S.E., Goudge M.C, & Paszek M.J. (2019). Mucin-Coating Technologies for Protection and Reduced Aggregation of Cellular Production Systems. Biotechnology and bioengineering, 116(5), 994-1005.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!