Brequinar

Brequinar and liproxstatin-1 (Lip-1) were obtained from MedChemExpress (Monmouth Junction, NJ, USA). Cisplatin (DDP) was acquired from Sigma (St. Louis, MO, USA). Brequinar was prepared in a range of concentrations (0.01, 0.1, 1, 10 and 100 μM) using dimethylsulfoxide (DMSO) and stored at −20 °C before use. CCK-8 kit was purchased from Dojindo (Kumamoto, Japan). The BCA protein assay kit was purchased from Beyotime (Shanghai, China). Primary antibodies for rabbit anti-human GAPDH, rabbit anti-human mTOR and rabbit anti-human p-mTOR were purchased from CST (Danvers, MA, USA). Primary antibodies for rabbit anti-human vinculin, rabbit anti-human Ki-67 and mouse anti-human 4-HNE were purchased from Abcam (Cambridge, UK). Primary antibody for rabbit anti-human DHODH was purchased by ABclonal (Wuhan, China). The second goat anti-rabbit and anti-mouse antibodies conjugated with peroxidase were purchased from Biosharp (Shanghai, China). DAB Substrate Kit was obtained from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China). The hematoxylin staining solution was obtained from Solarbio (Beijing, China). DAPI solution was purchased from Solarbio (China). The TUNEL detection kit was acquired from Roche (Basel, Switzerland).