Model 680 microplate reader

Manufactured by Bio-Rad

Sourced in United States, Japan, Italy, China, United Kingdom

The Model 680 Microplate Reader is a versatile laboratory instrument designed for absorbance-based measurements. It is capable of reading 96-well and 384-well microplates, making it suitable for a wide range of applications, including enzyme-linked immunosorbent assays (ELISA), cell-based assays, and colorimetric assays. The instrument features a temperature-controlled incubation chamber and supports multiple wavelength detection, enabling accurate and reliable data collection.

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Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by Merck Group (19 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (11 mentions) Dimethyl sulfoxide (dmso), by Merck Group (10 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (9 mentions) Celltiter 96 aqueous one solution cell proliferation assay, by Promega (8 mentions) Transdetect cck, by Transgene (8 mentions) Cck 8, by Dojindo Laboratories (7 mentions) Celltiter 96 proliferation assay kit, by Promega (6 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (6 mentions) 96 well plate, by Corning (6 mentions) 96 well microplate, by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin solution, by Merck Group (4 mentions) Cell proliferation reagent wst 1, by Roche (4 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (4 mentions) Si mir492, by GenePharma (4 mentions) Cck 8, by Bio-Rad (4 mentions) Cell count reagent sf, by Nacalai Tesque (4 mentions) Celltiter 96 aqueous non radioactive cell proliferation assay, by Promega (3 mentions) Microplate manager software, by Bio-Rad (3 mentions) Wst 1 reagent, by Roche (3 mentions)

Close spelling variants of this product

Microplate reader (6794 citations) Model 680 (943 citations) 680 microplate reader (215 citations) Model 680 Microplate (5 citations) Model 680 microplate reader S/N 22002 (4 citations) Microplate ReaderBio-Rad microplate reader (Model 680; (3 citations) Model 680-well microplate reader (2 citations) Bio-Rad Spectrophotometer Model 680 Microplate Reader (2 citations)

553 protocols using model 680 microplate reader

Mitochondrial Respiration and Protein Quantification

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Respiration data from the Seahorse experiments were normalized to citrate synthase activity and protein content. Citrate synthase activity was analyzed using Citrate Synthase Assay Kit (Sigma-Aldrich, St. Louis, US). Cells from all cell lines were resuspended in mitochondrial respiration medium (MiR05) (sucrose 110 mM, HEPES 20 mM, taurine 20 mM, K-lactobionate 60 mM, MgCl₂ 3 mM, KH₂PO₄ 10 mM, EGTA 0.5 mM, BSA 1 g/l) to a concentration of 3 x 10⁶ cells/mL, then sonicated using Ultrasonic homogenizer 4710 Series (Cole-Parmer Instrument, Vernon Hills, US). Samples were added to a 96-well plate together with 1x Assay buffer, 30 mM acetyl-coenzyme A and 10 mM 5,5’-Dithiobis-2-nitrobenzoic acid. 10 mM oxaloacetic acid was added and citrate synthase measured with BIO-RAD Microplate Reader Model 680 (Bio-Rad, Hercules, US).
Protein contents were analyzed with Bradford Protein Assay Kit II (Bio-Rad, Hercules, US). After each Seahorse experiment, the plates were washed with 1x phosphate-buffered saline and frozen at -80°C. Before assay, the cells were thawed and lysed with Radioimmunoprecipitation Assay lysis buffer. 10 µL of cell lysate was mixed with 190 µl Protein Assay Dye Reagent and transferred to assay plate. Absorbance was recorded using Microplate Reader Model 680 (Bio-Rad, Hercules, US) and compared against Bradford Assay Standards.

Li N., Chamkha I., Verma G., Swoboda S., Lindstedt M., Greiff L., Elmér E, & Ehinger J. (2024). Human papillomavirus-associated head and neck squamous cell carcinoma cells rely on glycolysis and display reduced oxidative phosphorylation. Frontiers in Oncology, 13, 1304106.

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Evaluating Cellular Cytotoxicity and Apoptosis

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Cells were assessed using an MTT assay. MTT solution (20 µl) was added to the cells after transfection at 24, 48 and 72 h. Following incubation for 4 h, the previous medium was removed and 150 ml dimethyl sulfoxide (DMSO) was added to the cells for 20 min at 4°C. The optical density (OD) was read at 570 nm using Bio-Rad Microplate Reader Model 680 (Bio-Rad Laboratories, Hercules, CA, USA).
To assess the LDH activity level after transfection at 24 h, the cells were harvested using an LDH level kit (Beyotime Institute of Biotechnology, Nanjing, China). The OD was read at 450 nm using Bio-Rad Microplate Reader Model 680 (Bio-Rad Laboratories).
To assess apoptosis using flow cytometry, after transfection at 24 h, the cells were harvested and stained with FITC-Annexin V and 7-AAD. The cells were analyzed with BD AccuriC6 (BD Biosciences) and data was analyzed using FlowJo software (FlowJo, LLC).

Wan J., Ling X., Peng B, & Ding G. (2018). miR-142-5p regulates CD4+ T cells in human non-small cell lung cancer through PD-L1 expression via the PTEN pathway. Oncology Reports, 40(1), 272-282.

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Graphene Oxide Cytotoxicity Evaluation

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The murine-derived macrophage cell line RAW 264.7 cells were purchase from American Type Culture Collection (ATCC). RAW 264.7 cells were cultured in 96-well plates (10³ cells/well) and treated with 0.1, 1, and 10 μg/mL GO in α-minimum essential medium (α-MEM) for 1 and 2 days. Cell proliferation was measured using a cell counting kit-8 assay (CCK-8; Dojindo, Kumamoto, Japan) and MTT (3-(4,5-dimethyl)thiazol-2-yl-2,5-dimethyltetrazolium bromide) following the manufacturer’s instructions. For CCK-8 assay, the medium was replaced with 100 μL of α-MEM and 10 μL of CCK-8 at each time point. After a 4-hour incubation at 37°C, the absorbance at 450 nm was determined using a model 680 microplate reader (Bio-Rad Laboratories Inc., Hercules, CA, USA). As for MTT, the medium was replaced with 200 μL MTT solution (5 mg/mL) at each time point. The cells were cultured for another 5 hours. During this period, viable cells could reduce the MTT to formazan pigment, which was dissolved by 800 μL dimethyl sulfoxide (DMSO) after removal of the culture medium. The absorbance at 490 nm was recorded using a model 680 microplate reader (Bio-Rad Laboratories Inc.).

Xue D., Chen E., Zhong H., Zhang W., Wang S., Joomun M.U., Yao T., Tan Y., Lin S., Zheng Q, & Pan Z. (2018). Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis. International Journal of Nanomedicine, 13, 5799-5810.

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Echinacoside Protects Against UVB-Induced Cell Damage

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Cell proliferation was assessed by MTT assay. Cells were grown in 96-well plates at a density of 1 × 10⁵ cells/mL. At a confluence of 80–90%, cells were pretreated with various concentrations (25, 50, and 100 μmol/L medium) of echinacoside in FBS-free RPMI 1640 medium. After incubation for 24 h, they were washed with PBS and stimulated by UVB irradiation in 200 μL PBS. Subsequently, cells were grown in fresh serum-free medium and incubated for 24 h. The supernatant was removed and 30 μL MTT (5 mg/mL in PBS) was added to each plate and incubated for another 4 h. Then, the supernatant was discarded, and 150 μL of dimethyl sulfoxide was added to dissolve the formazan crystals. The absorbance of each sample was recorded at 490 nm with a Model 680 Microplate Reader (Bio-Rad, Hercules, CA, USA).
Cell injury was measured by quantifying the amount of LDH, a cytosolic enzyme released in the supernatant of cultures by damaged cells. The conditioned media of the UVB-exposed cells were collected for LDH measurement following the supplier's instructions (Cat number A020, Nanjing Jiancheng Bioengineering Institute, China). The absorbance was determined at a wavelength of 450 nm immediately using a Model 680 Microplate Reader (Bio-Rad, Hercules, CA, USA).

Zhang D., Lu C., Yu Z., Wang X., Yan L., Zhang J., Li H., Wang J, & Wen A. (2017). Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis. Oxidative Medicine and Cellular Longevity, 2017, 6851464.

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Cell Viability Assay in 96-well Plates

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Cells were seeded in 96-well plates at a density of 2×10³ cells/well, and cell viability was assessed using the CCK-8 Assay Kit (Dojindo Molecular Technologies, Japan), as instructed by the manufacturer. Samples were measured at OD 490 nm using the Bio-Rad Microplate Reader Model 680 (Bio-Rad, China).

Wu S., Ren K., Zhao J., Li J., Jia B., Wu X., Dou Y., Fei X., Huan Y., He X., Wang T., Lv W., Wang L., Wang Y., Zhao J., Fei Z, & Li S. (2022). LncRNA GAS5 represses stemness and malignancy of gliomas via elevating the SPACA6-miR-125a/let-7e Axis. Frontiers in Oncology, 12, 803652.

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Cytotoxicity Assay for p53-Dependent Cancer Cells

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HCT116^p53+/+ and HCT116^p53−/− cells were seeded into the 96-well plate at a density of 1 × 10⁴ cells/well and incubated overnight at 37 °C with 5% CO₂. Then, they were treated with various concentrations of OP-D for 24 h. After that, the Cell Counting Kit-8 (CCK-8) (Dojindo Molecular Technologies, Rockville, MD, United States) was distributed to each well and incubated for 1 h at 37°C with 5% CO₂ and the absorbance of the samples was measured in a BioRad microplate reader model 680 (Biorad, Hercules, CA, United States) at 450 nm.

Ko H.M., Jee W., Lee D., Jang H.J, & Jung J.H. (2022). Ophiopogonin D increase apoptosis by activating p53 via ribosomal protein L5 and L11 and inhibiting the expression of c-Myc via CNOT2. Frontiers in Pharmacology, 13, 974468.

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Anticancer Potency Evaluation in Colon Cancer Cells

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To determine cancer cell response to chemotherapeutics and other compounds in targeted screenings, as well as to explore colon cancer signaling pathways, we tested the anticancer activity of samples in HCT116 cells. After 72 h of cell exposure to samples, the activity was evaluated and in parallel to the positive and vehicle controls. CellTiter 96^® aqueous non-radioactive cell proliferation assay (Promega, Madison, WI, USA) was used to anticancer activity evaluation using 4-(4,5-dimethylathiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl), inner salt (MTS), according to the manufacturer‘s instructions. And the quantity of formazan product was measured after 1 h of incubation, using a Bio-Rad microplate reader Model 680 (BioRad, Hercules, CA, USA) at 490 nm. GraphPad Prism (version 5 GraphPad Software) was used to the IC₅₀ values determination. All samples were diluted, resulting in final concentrations of the tested samples ranging from 156.2 to 0.08 µg mL⁻¹.

Cruz S., Gomes S.E., Borralho P.M., Rodrigues C.M., Gaudêncio S.P, & Pereira F. (2018). In Silico HCT116 Human Colon Cancer Cell-Based Models En Route to the Discovery of Lead-Like Anticancer Drugs. Biomolecules, 8(3), 56.

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Cytotoxicity Assessment of HA+AA

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NHOst cells (3 × 10³ per well) were seeded in 96-well plates and cultured for 24 h. Then, six dilutions of the HA+AA solution were added in triplicate. After three and four days, the medium with HA+AA was removed and replaced with fresh culture medium containing 10 µL of MTT solution (5 mg/mL), as described elsewhere [21 (link)]. The plates were incubated for 3 h at 37 °C; then, the formazan crystals were dissolved adding a solution of HCl 0.01 M and SDS 10% and incubated for 24 h at 37 °C. The Bio-Rad microplate reader model 680 (Bio-Rad Laboratories, Segrate, MI, Italy) was exploited to measure the absorbance at 570 nm. The results are reported as mean ± standard deviation and were obtained via six independent assays.

Bonifacio M.A., Cassano A., Vincenti A., Vinella A., Dell’Olio F., Favia G, & Mariggiò M.A. (2023). In Vitro Evaluation of the Effects of Hyaluronic Acid and an Aminoacidic Pool on Human Osteoblasts. Biomedicines, 11(3), 751.

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Folin-Ciocalteu Assay for Total Phenolic Content

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Total phenolic content of the extracts was determined by the Folin-Ciocalteau method described by [55 (link)], adapted to microscale. In brief, 5 μL of each extract at a concentration of 50 mg/mL was mixed with 50 μL of distilled water and 100 μL of 10% Folin–Ciocalteu reagent. The solution was stirred and after 3 min, 200 μL 10% sodium carbonate solution was added. Samples were left in the dark at room temperature for 1 h. The absorbance was read at 750 nm with a BioRad Microplate Reader Model 680 (Bio-Rad Laboratories, Inc., Hercules, CA, USA). The same process was repeated for gallic acid at final concentrations ranging from 0 to 500 μg/mL, and the obtained values were used to plot a calibration curve. TPC of the extracts was obtained by interpolation from the gallic acid calibration curve, and results were expressed as gallic acid equivalents in μg/mg of dried extract (μg GAE/mg).

Rosa G.P., Peixoto A.F., Barreto M.C., Seca A.M, & Pinto D.C. (2022). Bio-Guided Optimization of Cystoseira abies-marina Cosmeceuticals Extraction by Advanced Technologies. Marine Drugs, 21(1), 35.

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DPPH Radical Scavenging Antioxidant Assay

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Antioxidant activity was assayed by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay [53 (link)]. Serial dilutions of studied extracts, or reference compound (Trolox) were carried out in 96-well microplates, with concentrations ranging between 0.244 μg/mL and 250 μg/mL in methanol. DPPH dissolved in methanol was added to the microwells, yielding a final concentration of 45 µg/mL, and the absorbance at 515 nm was measured with a BioRad Microplate Reader Model 680 (Bio-Rad Laboratories, Inc., Hercules, CA, USA), after 30 min in the dark. In each assay, a control was prepared, in which the same amount of solvent substituted the sample or standard. Percentage of antioxidant activity (% AA) was calculated as:
where Abs_control is the absorbance of the control and Abs_sample is the absorbance of the alga extract or standard. All assays were carried out in triplicate and the results expressed as IC₅₀, i.e., as the concentration yielding 50% scavenging of DPPH, calculated by interpolation from the% AA vs. concentration curve.

Rosa G.P., Peixoto A.F., Barreto M.C., Seca A.M, & Pinto D.C. (2022). Bio-Guided Optimization of Cystoseira abies-marina Cosmeceuticals Extraction by Advanced Technologies. Marine Drugs, 21(1), 35.

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