Htb 38

Human cell lines glioma U87-MG (ATCC^® HTB-14^™), astrocytoma 1321N1 (ATCC LH-1), lung adenocarcinoma A549 (ATCC CCL-185), mamma carcinoma MDA-MB-231 (ATCC HTB-26) and JIMT-1 (DSMZ, ACC 589) were cultured in DMEM, colorectal adenocarcinoma HT-29 (ATCC^® HTB-38^™) in F12 DMEM; all supplemented with 10% FCS, 100 U penicillin, and 100 μg streptomycin/ml. U87-MG were cultured in DMEM as described above and supplemented with 1% non-essential amino acids. Prostate carcinoma PC-3 (ATCC CRL-1435), LnCaP (DSMZ; ACC 256), acute monocytic leukaemia THP-1 (ATCC LH-1), and chronic myelogenous leukaemia K562 (ATCC^® HTB-38^™) were cultured in RPMI supplemented with 10% FCS, 100 U penicillin, and 100 μg streptomycin/ml, whereas HNSCC FaDu (ATCC HTB-43) and HN-5 (Boehringer Ingelheim) were cultured in phenol red- and riboflavin-free RPMI 1640 supplemented with antibiotics. All cell lines were cultured at 37°C/5% CO₂ in humidified atmosphere (standard conditions).

The HT-29 human colorectal adenocarcinoma cell line was obtained from the American Type Culture Collection (ATCC #HTB-38) and was maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 0.05 mg/mL gentamicin (Sigma-Aldrich, MO, USA) and were grown in 75 cm² tissue culture flasks (Greiner-Bio-One, NC, USA) at 37°C, 5% CO₂ in a humidified incubator (Thermo Fisher, MA, USA). Cells were passaged every 3 days or when cells reached ~90% confluency. Cell passages 12–28 were used for all assays.
The non-transformed rat IEC-6 (ATCC #CRL-1592) small IEC line was maintained in DMEM supplemented with 10% FBS and 0.05 mg/mL gentamicin, and cell cultures were maintained in a similar manner to HT-29 cells. Cell passages 6–15 were used for all assays.

Lung cancer cell lines NCI-H460 (ATCC^® HTB-1770™) and A549 (ATCC^® CCL-185™), breast cancer cell lines MCF7 (ATCC^® HTB-22™) and MDA-MB-231 (ATCC^® HTB-26™), and colon carcinoma cell lines SW620 (ATCC^® CCL-227™) and HT-29 (ATCC^® HTB-38™) were purchased from ATCC (Rockville, MD). NCI-H460 and HT-29 cell lines were grown in RPMI 1640 medium (Corning Life Sciences, VWR International, Radnor, PA) supplemented with 2 mM l-glutamine and 10% fetal bovine serum. MDA-MB-231, MCF7, and SW620 cell lines were grown in DMEM medium (Gibco, Thermo Fisher, Waltham, MA) supplemented with 4 mM l-glutamine and 10% fetal bovine serum. A549 cells were grown in DMEM/F-12 medium (Gibco, Thermo Fisher, Waltham, MA) supplemented with 4 mM l-glutamine and 10% fetal bovine serum. l-glutamine and fetal bovine serum were purchased from Thermo Fisher (Waltham, MA). The media was renewed every 2–3 days. For subculturing, cells were detached using Accutase (Stem Cell Technology, Vancouver, Canada) and seeded in 1:3 or 1:6 ratios. All cells were cultured at 37 °C with 5% CO₂. All cell lines were authenticated by short tandem repeat (STR) analysis at the University of Arizona Genetics Core Facility during our study.