Sodium taurocholate, cholesterol, sodium chloride (NaCl), sodium oleate, ammonium formate, potassium hydroxide (KOH), hydrochloric acid (HCl), naproxen, phenytoin, piroxicam, fenofibrate, probucol, griseofulvin, carvedilol, tadalafil, and indomethacin were purchased from Merck Chemicals Ltd. Aprepitant and felodipine were provided through OrBiTo by Dr. R. Holm, Head of Preformulation, Lundbeck, Denmark. Zafirlukast was purchased from Stratech Scientific Ltd. Phosphatidylcholine from soybean (lecithin) was purchased from Lipoid company. Chloroform from Rathburn Chemical Company. FaSSIF-v1 media was purchased from Biorelevant.com Ltd. Sodium phosphate monobasic monohydrate (NaH2PO4·H2O) and formic acid from Fisher Scientific. All acetonitrile (ACN) and methanol (MeOH) solvents were HPLC gradient (VWR). All water was ultrapure Milli-Q.
Carvedilol
Manufactured by Merck Group
Sourced in United States, Germany, Macao, Hungary, France
Carvedilol is a pharmaceutical compound used in the manufacturing of various lab equipment. It serves as a core component in the production of these devices.
Automatically generated - may contain errors
Lab products found in correlation
Metoprolol, by Merck Group (5 mentions)
Atenolol, by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Piroxicam, by Merck Group (3 mentions)
Imipramine hydrochloride, by Merck Group (3 mentions)
Phenytoin, by Merck Group (2 mentions)
Tadalafil, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Nebivolol, by Merck Group (2 mentions)
Forskolin, by Merck Group (2 mentions)
Propranolol, by Merck Group (2 mentions)
Amiodarone hydrochloride, by Bio-Techne (2 mentions)
Thioridazine hydrochloride, by Merck Group (2 mentions)
Recombinant human tgf β1, by Thermo Fisher Scientific (2 mentions)
Nifedipine, by Merck Group (2 mentions)
Propranolol hydrochloride, by Merck Group (2 mentions)
Isoproterenol hydrochloride, by Merck Group (2 mentions)
Isoproterenol, by Merck Group (2 mentions)
Pindolol, by Merck Group (2 mentions)
Bisoprolol, by Merck Group (2 mentions)
41 protocols using carvedilol
1
Evaluating Solubility of Diverse Pharmaceutical Compounds
2
Pharmaceutical Compound Characterization Protocol
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Active pharmaceutical ingredients of studied medicines, i.e., atenolol (Daroupakhsh, Iran), benzoic acid (Merck, Iran), carbamazepine (Arastoo, Iran), carvedilol (Salehan Shimi, Iran), Ibuprofen (Daana, Iran), ketoconazole (Arastoo, Iran), lamotrigine (Arastoo, Iran), phenothiazine (Merck, Germany), phenytoin (Alhavi, Iran), piroxicam (Zahravi, Iran) salicylic acid (Merck, Germany), sulfamethoxazole (Merck, Germany), and tadalafil (Osveh, Iran), were provided from pharmaceutical and chemical companies (Purity: > 99%). Ethanol (96% w/w) was purchased from Jahan Alcohol (Iran), and choline chloride (> 99%), glycerol (> 99%), and urea (> 99%) from Merck (Germany). Lab-made double distilled water was used for the preparation of solutions.
3
HPLC Enantioseparation of Carvedilol
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Carvedilol, HPLC-grade solvents (n-hexane, 2-propanol (IPA), ethanol, ethyl acetate (EA), dichloromethane (DCM), acetonitrile (ACN) and amines (diethylamine (DEA), triethylamine, buthylamine, exylamine, ethanolamine, ethylenediamine and ethylamine) were purchased from Sigma-Aldrich (Milan, Italy). HPLC enantioseparations were performed by using stainless-steel Chiralpak® IG-3 (250 mm × 4.6 mm, 3 μm), Chiralpak® IG (250 mm × 10 mm, 5 μm), Chiralpak® IA-3 (250 mm × 4.6 mm, 3 μm) and Chiralpak® IA (250 mm × 10 mm, 5 μm) columns (Chiral Technologies Europe, Illkirch, France).
4
Compound Preparation for Cell Studies
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The β-blockers were resuspended in dimethyl sulfoxide (DMSO). Stock solutions were stored at -20°C for Nebivolol (50 mM, Sigma-Aldrich, ref. #N1915) and Carvedilol (50 mM, Sigma-Aldrich, ref. #C3993) and at -80°C for Propranolol (150 mM, Selleckchem, ref. S4076). The antioxidants Mito-TEMPO (MT) and Troxerutin (TROX) were resuspended in DMSO and stock at -20°C. The solutions are diluted in culture medium ex-temporaneously for the experiments.
5
Investigating Cardiac Signaling Pathways
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Isoprenaline and propranolol , carvedilol , atenolol , and ICI‐118551 were supplied by Sigma. These compounds were dissolved in saline containing 0.4‐mM ascorbic acid and administered through an osmotic minipump (ALZET model 2001, Cupertino, CA) subcutaneously implanted under isoflurane anaesthesia. Sigma also supplied forskolin (#F3917), the PKA inhibitor H89 (#B1427) and the PKA inhibitor, fragment 14–22 (PKI, #P9115). We used antibodies against Mst1 (Cell Signaling Technology Cat# 3682, RRID:AB_2144632), yes‐associated protein (YAP, Cell Signaling Technology Cat#12395), p‐YAP (ser127; Cell Signaling Technology Cat# 13008, RRID:AB_2650553), BIM (Cell Signaling Technology Cat# 2933, RRID:AB_1030947), Gal‐3 (Thermo Fisher Scientific Cat# MA1‐940, RRID:AB_2136775), Lamin A/C (E‐1; Santa Cruz Biotechnology (Dallas, TX), Cat# sc‐376248, RRID:AB_10991536), GAPDH (6C5; Santa Cruz Biotechnology Cat# sc‐32233, RRID:AB_627679).
6
Carvedilol and Metoprolol Quantification
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Carvedilol, metoprolol, sodium chloride, potassium phosphate monobasic, and sodium phosphate dibasic, hexadecane, octanol, and trifluoroacetic acid (TFA) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Water and acetonitrile (Merck KGaA, Darmstadt, Germany) were ultra-performance liquid chromatography (UPLC) purity grade. All other substances were of analytical reagent grade.
7
Evaluation of Pharmacological Agents
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Doxazosin, carvedilol, and curcumin were purchased from Sigma Chemical Company (St. Louis, MO, USA). Carbon tetrachloride and formaldehyde were obtained from J.T. Backer (Xalostoc, Mexico). Pentobarbital was obtained from MAVER Laboratories.
8
Cellular Adhesion and Signaling Assay
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Reagents were purchased from Fisher Scientific (Hampton, NH) or Sigma (St. Louis, MO) unless otherwise specified. All reagents were validated by the manufacturer and/or have been previously cited in the literature. Antibodies used were: anti-α-Tubulin (T9026; Sigma, St. Louis, MO), anti-pFAK397 (3283; Cell Signalling Technology, Danvers, MA), anti-pPaxillin118 (44–722 G; ThermoFisher Scientific, Waltham, MA), anti-cadherin-11 (71–7600; ThermoFisher Scientific, Waltham, MA), anti–phospho-p44/42 ERK (Thr202/Tyr204; 4370; Cell Signalling Technology, Danvers, MA), anti-p44/42 ERK (9102; Cell Signalling Technology, Danvers, MA), anti-Ki67 (ab15580; Abcam, Cambridge, UK), AlexaFluor 488 anti-rabbit secondary (A-11,008; ThermoFisher Scientific, Waltham, MA), and AlexaFluor 647 anti-rabbit secondary (A-21,244; ThermoFisher Scientific, Waltham, MA). ECM substrates used were: Collagen I (CB-40,236; Fisher Scientific, Hampton, NH). Inhibitors used were: Amiodarone hydrochloride (40–955–0; Tocris Bioscience, Minneapolis, MN), Carvedilol (C3993, Sigma, St. Louis, MO), Imipramine hydrochloride (I7379; Sigma, St. Louis, MO), and Thioridazine hydrochloride (T9025, Sigma, St. Louis, MO).
9
Cell Culture Reagents and Treatments
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Carvedilol, 3,4-dihydroxy-L-phenylalanine (L-DOPA), cholera toxin (CT), and 12-O-tetradecanoylphorbol-13-acetate (TPA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s Medium (DMEM) and Dulbecco’s phosphate-buffered saline were purchased from WelGENE (Daegu, Korea). Fetal bovine serum (FBS), antibiotic-antimycotic, and trypsin-EDTA were purchased from Gibco (Grand Island, NY, USA). Medium 254 (Cascade Biologics, Portland, OR, USA) and FSK ([3R-(3α,4aβ,5β,6β,6aα,10α,10aβ,10bα)]-5-(Acetyloxy)-3-ethenyldodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1H-naphtho[2,1-b]pyran-1-one) were purchased from Tocris Bioscience (Bristol, UK).
10
Culturing HUVECs under TGFβ1 treatment
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Human umbilical vein endothelial cells (HUVECs) were obtained from ATCC (Manassas, VA, USA) and cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA), 100 mg/mL streptomycin, and 100 U/mL penicillin at 37°C in a humidified incubator with 5% CO2.
HUVECs were treated with or without the TGFβ1 and different reagents. Carvedilol was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Recombinant human TGFβ1 was obtained from PeproTech (Rocky Hill, NJ, USA). The inhibitors PD98059 and SIS3 were both purchased from MedChem Express (Monmouth Junction, NJ, USA).
HUVECs were treated with or without the TGFβ1 and different reagents. Carvedilol was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Recombinant human TGFβ1 was obtained from PeproTech (Rocky Hill, NJ, USA). The inhibitors PD98059 and SIS3 were both purchased from MedChem Express (Monmouth Junction, NJ, USA).
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