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Vevo lab version 3

Manufactured by Fujifilm

Vevo LAB version 3.1.0 is a high-performance, small-animal imaging system designed for in vivo, non-invasive, and real-time visualization and analysis of biological structures and functions. It provides advanced imaging capabilities for a wide range of applications, including cardiovascular, oncology, and neuroscience research.

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3 protocols using vevo lab version 3

1

Comprehensive Cardiac Imaging Assessment

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Left ventricular volumes, ejection fraction and cardiac output were calculated from B-mode images using the semi-automated LVtrace-tool of Vevo LAB version 3.1.0 (FUJIFILM VisualSonics). All B-mode loops were traced twice to account for inter-beat variability. Peak velocities for E and A were assessed from pulsed-wave Doppler transmittal flow patterns and e′ from tissue Doppler profiles of five peaks.
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2

Comprehensive Cardiac Imaging Assessment

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Left ventricular volumes, ejection fraction and cardiac output were calculated from B-mode images using the semi-automated LVtrace-tool of Vevo LAB version 3.1.0 (FUJIFILM VisualSonics). All B-mode loops were traced twice to account for inter-beat variability. Peak velocities for E and A were assessed from pulsed-wave Doppler transmittal flow patterns and e′ from tissue Doppler profiles of five peaks.
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3

Comprehensive Cardiac and Aortic Imaging in Mice

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Echocardiography of both ventricles, aorta, and lung was performed using a Vevo 3100 high‐resolution Imaging System coupled to a MX400 ultra‐high‐frequency linear array transducer (18–38 MHz; center transmit: 30 MHz; axial resolution: 50 μm) (both FUJIFILM VisualSonics, Toronto, Ontario, Canada), as described previously.22, 23 Briefly, mice were exposed to 3% isoflurane (Baxter International, Deerfield, IL) and fixed in a dorsal position on a heating pad to maintain physiological body temperatures.
Assessment of AoVs and 3‐dimensional echocardiography of the aortic arch were performed using the MX700 ultra‐high‐frequency linear array transducer (30–70 MHz; center transmit: 50 MHz; axial resolution: 30 μm; FUJIFILM VisualSonics). The 3‐dimensional image acquisition was realized with a specialized 3‐dimensional motor (FUJIFILM VisualSonics), allowing for automated stepwise movement of the probe, as described previously by us.22 All acquired images were digitally stored in raw format (DICOM) for further offline analyses. Image analyses were performed by a trained expert in small animal echocardiography using the dedicated software package VevoLAB Version 3.1.0 (FUJIFILM VisualSonics).
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