The analytical grade chemicals were purchased from Hi-Media and Merck, India. Standard drugs were purchased from Sigma-Aldrich, India. 2,4,6-tris(2-pyridyl)-s-triazine TPTZ and MTT reagent were procured from Merck, India. Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS) and penicillin (5000 U/mL), streptomycin (2500 U/mL) were purchased from Gibco (USA).
2 4 6 tris 2 pyridyl s triazine tptz
Manufactured by Merck Group
Sourced in United States, Germany, Italy, Spain, China
2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) is a chemical compound commonly used as a reagent in laboratory settings. It serves as a complexing agent, forming colored complexes with various metal ions, particularly iron(II). The core function of TPTZ is to provide a means for the detection and quantification of these metal ions through colorimetric analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (26 mentions)
Gallic acid, by Merck Group (16 mentions)
Folin ciocalteu reagent, by Merck Group (13 mentions)
Acetic acid, by Merck Group (9 mentions)
Trolox, by Merck Group (9 mentions)
Methanol, by Merck Group (9 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions)
Hydrochloric acid (hcl), by Merck Group (8 mentions)
Quercetin, by Merck Group (7 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid, by Merck Group (7 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (7 mentions)
Sodium acetate, by Merck Group (6 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (6 mentions)
Sodium carbonate, by Merck Group (6 mentions)
Feso4 7h2o, by Merck Group (6 mentions)
Ferulic acid, by Merck Group (6 mentions)
P coumaric acid, by Merck Group (5 mentions)
Caffeic acid, by Merck Group (5 mentions)
L ascorbic acid, by Avantor (5 mentions)
Fecl3 6h2o, by Merck Group (5 mentions)
103 protocols using 2 4 6 tris 2 pyridyl s triazine tptz
1
Analytical Reagents and Cell Culture Materials
2
Cytotoxicity Assessment of Cisplatin
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Fetal bovine serum (FBS), Roswell Park Memorial Institute (RPMI) 1640 medium, penicillin, streptomycin, and trypsin were provided by Gibco BRL (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Pen-Strep (penicillin-streptomycin), ethanol, 5,5-dithiobis-2-nitrobenzoic (DTNB), ethylenediaminetetraacetic acid (EDTA), and cisplatin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Other materials such as trichloroacetic acid (TCA), 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were purchased from Merck (Germany). All chemicals and reagents were used without any further purification. All procedures were accepted by the Ethics Committee of Yasuj University of Medical Sciences (ethical code: IR.YUMS.REC.1398.114).
3
MRS Broth Preparation and Reagents
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De Man, rogosa & sharpe (MRS) broth medium was prepared from Himedia (India). Cisplatin was obtained from Ebewe Pharma (Austria). Sodium thiopental, tris, potassium chloride (KCl), dichlorofluorescein (DCF), phosphoric acid, thiobarbituric acid, n-butanol, trichloroAcetic acid (TCA), ethylenediaminetetraAcetic acid (EDTA), and dithio-bis-(2-nitrobenzoic acid) (DTNB, Ellman’s reagent) were purchased from Merck (Germany). Acetic acid, sodium acetate, ferric chloride dihydrate, and 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ) were also from Merck (Germany). All other solvents and reagents utilized to prepare various buffer solutions were of analytical grade and purchased from Merck (Germany). All the preparations were made using deionized water (Direct Q UV3, Millipore, USA).
4
Comprehensive Chemical Reagent Database
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Bovine serum albumin (BSA), Tris, HEPES-NaOH, disodium edetate, theophylline, TEP, trichloroacetic acid (TCA), 2-thiobarbituric acid (TBA), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), glucose, fructose and 0.1 M phosphate buffer (PBS) were purchased from Sigma-Aldrich Chemie GmbH (Taufkirchen, Germany). 1-deoxy-1-morpholino-fructose (1-DMF) and nitroblue-tetrazolium (NBT) were purchased from BLD Pharmatech GmbH (Kaiserslautern, Germany). Erythritol was purchased from SHG GROUP (Pustynia, Poland). Xylitol was purchased from Nanga (Złotów, Poland) and collagen was from Hyphen Biomed (Neuville-sur-Oise, France). KCl, Na2CO3, NaCl, CaCl2, KH2PO4, MgCl × 6 H2O, NaHCO3, FeCl3 × 6 H2O, FeSO4 × 7 H2, sodium citrate and citric acid were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland).
5
Antioxidant and Genotoxicity Assays
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Betulinic acid (BA, CAS no. 472-15-1), 1, 1-diphenyl-2-picrylhydrazyl free radical (DPPH, CAS no. 1707-75-1), 2,4,6-Tris (2-pyridyl)-s-triazine (TPTZ, CAS no. 3682-35-7), triton X-100 (CAS no. 9002-93-1) and methyl methanesulfonate (MMS, CAS no. 66-27-3) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Ethanol and aluminium chloride anhydrous (AlCl3, CAS no. 7446-70-0) were purchased from Merck Specialties’ (Mumbai, India). Acetonitrile (HPLC grade) and dimethylsulfoxide (DMSO) was procured from Qualigens (Mumbai, India). Other reagents like ethylene diamine tetra acetic acid (EDTA) di-sodium salt, normal melting point agarose (NMPA) and low melting point agarose (LMPA), HiSep™ LSM 1084, Tris buffer, phosphate-buffered saline (PBS) (Ca2+, Mg2+ free), RPMI-1640 media, were procured from Hi Media, Mumbai, India. Ascorbic acid, gallic acid, Folin–Ciocalteu reagents were purchased from SRL (Mumbai, India). Sodium di-hydrogen phosphate (NaH2PO4), di-sodium hydrogen phosphate (Na2HPO4), hydrogen peroxide (H2O2), ferric chloride (FeCl3), sodium hydroxide (NaOH) and sodium carbonate (Na2CO3) were obtained locally and were of analytical grade.
6
Antimicrobial and Antioxidant Properties Evaluation
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Saline peptone water, Brain Heart Infusion (BHI), Tryptic Soy Broth (TSB) supplemented with Yeast, Plate Count Agar (PCA), Tryptone Sulphite Neomycin (TSN), Dextrose Sabouraud (DS), and Mannitol Egg Yolk Polymyxin (MYP) mediums were obtained from Merck KGaA, (Darmstadt, Germany). Acetonitrile; amphotericin B solution (250 μg/mL); 2,2′-Azinobis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS); Fetal Bovine Serum (FBS); glacial acetic acid; hydrochloric acid; 6-hydroxy 2,5,7,8-tetramethyl-2-carboxylic acid (Trolox); L-glutamine solution (200 mM); MEM (Minimum Essential Medium); methanol; penicillin (10,000 U/mL); pure phenolic compounds (caffeic acid, catechin, epicatechin, epicatechin gallate, ethyl gallate, gallic acid, gentisic acid, p-coumaric acid, procyanidin B1, procyanidin B2, protocatechuic acid, syringic acid, t-resveratrol, t-piceid, and vallinic acid); streptomycin solution (P/S; 100 mg/mL); and 2,4,6-Tris (2-pyridyl)-S-triazine (TPTZ) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Folin–Ciocalteu (FC) reagent, 70% (v/v), iron (III)-chloride acid (FeCl3), iron (II)-sulphate (FeSO4), sodium acetate (NaC2H3O2), and sodium carbonate (Na2CO3) were purchased from Panreac Quimica S.L.U. (Barcelona, Spain). Malvidin-3-glucoside was obtained from Estrasynthese (Genay, France).
7
Reagent Preparation for Biochemical Assays
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Acetaminophen (98% purity, CAS#: 103-90-2), 5,5′-Dithiobis (2-nitrobenzoic acid) (DTNB), N-(1-naphthyl) ethylenediamine dihydrochloride (NED), bovine serum albumin (BSA), 2-thiobarbituric acid (TBA), and 2,4,6-Tris (2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA).
8
HPLC Standards and Antioxidant Assays
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The high-performance liquid chromatography (HPLC) standards (chlorogenic acid, rutin) were purchased from Chemfaces (Wuhan, China). Antioxidant assay reagents: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2′,7′-dichlorofluorescin diacetate (DCFH-DA), trolox, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), quercetin, aluminum chloride hexahydrate (AlCl3·6H2O), potassium persulfate, and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture reagents: Dulbecco’s modification of Eagle’s medium (DMEM), penicillin/streptomycin antibiotic mix, glutamine, and fetal bovine serum (FBS) were purchased from Welgene (Gyeongsan, Korea). All other chemicals used were of 99% purity or higher.
9
Antioxidant Compounds Characterization
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All chemicals and reagents used in this study were of analytical grade unless stated otherwise. Trolox, gallic acid, Folin–Ciocalteu phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and 2,4,6-tris (2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Standard compounds such as hydroxytyrosol, esculin, taxifolin, luteolin, quercetin, kaempferol, apigenin, chlorogenic acid, plantamajoside, rutin, eriodictyol, tiliroside, apigenin-7-O-neohesperidoside, apigenin-7-O-glucoside, luteolin-7-O-glucoside, oleuropein, secoxyloganin, asiatic acid, oleanonic acid, maslinic acid, corosolic acid, oleanolic acid, and ursolic acid were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). For chromatography analysis, HPLC-grade acetonitrile and acetic acid were purchased from Merck (Darmstadt, Germany) and Alfa Aesar (Shanghai, China), respectively. Ultrapure water supplied by a Milli-Q system (Millipore, Bedford, MA, USA) was used throughout the experiments.
10
Phytochemical Profiling and Antioxidant Evaluation
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All the employed solvents were of analytical grade and purchased from Carlo Erba (Milan, Italy). Chlorogenic acid (5-CQA) and neochlorogenic acid (3-CQA) standards were purchased from Sigma Aldrich (Milan, Italy), while cryptochlorogenic acid (4-CQA) and caffeic acid (CA) standards were purchased from PhytoLab (Vestenbergsgreuth, Germany). Acetonitrile (ACN) and methanol, both LC-MS grade, were from Merck KGaA (Darmstadt, Germany). Formic acid for LC-MS was purchased from Sigma-Aldrich (St. Louis, MO, USA). Folin–Ciocalteu reagent, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonate) diammonium salt (ABTS), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethyl-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric chloride (FeCl3), and gallic acid (GA) were purchased from Sigma-Aldrich (Milan, Italy). Ultra-pure deionized water was generated by a Milli-Q purification apparatus (Millipore, Bedford, MA, USA).
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