Penicillin

Manufactured by Biosera

Sourced in France, United States, United Kingdom, Germany

Penicillin is a type of lab equipment used for the production and purification of the antibiotic penicillin. It serves as a controlled environment for the cultivation and extraction of the penicillin compound.

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Lab products found in correlation

79 protocols using penicillin

Cultivation of Healthy and Cancerous Human Cell Lines

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Four different commercially available human cell lines were used in this study. Two lines represented healthy cell types. Human dermal fibroblasts (HDFa) (Gibco, USA), were grown in DMEM:F12 (1:1) + Glutamax (Gibco, USA) culture medium, supplemented with 10% FBS (Biosera, GB) and 100 I.U./mL of penicillin and 0.1 mg/mL of streptomycin (Biosera, GB). Normal human astrocytes (NHA) (ScienCell, Carlsbad, CA, USA), were cultivated in Astrocyte Media (ScienCell, USA) supplemented with 2% FBS (ScienCell, USA), 1% AGS (100×; ScienCell, USA) and 100 I.U./mL of penicillin and 0.1 mg/mL of streptomycin (Biosera, GB).
The other two cell lines represented tumor cell types. Human neuroblastoma cell line (SH-SY5Y) (ECACC, UK), was cultivated in DMEM:F12 (1:1) + Glutamax (Gibco, USA), supplemented with 10% FBS (Biosera, GB) and 100 I.U./mL of penicillin and 0.1 mg/mL of streptomycin (Biosera, GB). Human glioblastoma cell line (T98G) (ECACC, UK), was cultivated in DMEM high glucose medium (Sigma-Aldrich, USA), supplemented with 10% FBS (Biosera, GB) and 100 I.U./mL of penicillin and 0.1 mg/mL of streptomycin (Biosera, GB).
Cells were cultivated at standard conditions (5% CO₂, 37 °C, humidified atmosphere). HDFa and T98G were plated at the density of 6.3 × 10³ cells/cm², NHA and SH-SY5Y were plated at the density of 9.4 × 10³ cells/cm².

Zahumenska R., Badurova B., Pavelek M., Sojka P., Pavlisova T., Spanik P., Sivonova M.K., Novakova S., Strnadel J., Halasova E., Frivaldsky M, & Skovierova H. (2024). Comparison of pulsed and continuous electromagnetic field generated by WPT system on human dermal and neural cells. Scientific Reports, 14, 5514.

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Cultivation and Maintenance of Colorectal Cancer Cell Lines

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Human colorectal carcinoma cell lines DLD1 and LoVo were obtained from the ATCC (USA). DLD1 cells were cultured in RPMI 1640 GlutaMax medium (Gibco, USA) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin (Biosera, France). LoVo cells were cultured in F-12K medium (Bioconcept, Switzerland) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin (Biosera, France). Cells were cultivated at 37°C in a HF90 humidified incubator (Heal Force, China) containing 5% CO₂. Experiments were performed three times.

Moravčík R., Olejárová S., Zlacká J, & Herichová I. (2023). Effect of miR-34a on the expression of clock and clock-controlled genes in DLD1 and Lovo human cancer cells with different backgrounds with respect to p53 functionality and 17β-estradiol-mediated regulation. PLOS ONE, 18(10), e0292880.

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Cultivation and Maintenance of Colorectal Cancer Cell Lines

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Cultivation and Viability Assessment of Cell Lines

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THP-1-XBlue-MD2-CD14 cells were supplied by Invivogen (San Diego, CA, USA) and cultured in RPMI 1640 medium containing stabilized 2 mM l-glutamine (Biosera, Nuaille, France), supplemented with antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin (Biosera), and 10% fetal bovine serum (FBS) (HyClone, Logan, UT, USA). The cells were kept in an incubator at 37 °C in a water-saturated atmosphere of air containing 5% CO₂. The suspensions of THP-1-XBlue-MD2-CD14 cells were passaged approximately twice a week.
The HepG2 human hepatoma cell line was purchased from the European Collection of Cell Cultures (Salisbury, UK). Cells were grown in DMEM low glucose medium (Biosera) supplemented with antibiotics (100 U/mL penicillin, 100 mg/mL streptomycin), 10% FBS, and 2 mM l-glutamine. Cultures were kept in an incubator at 37 °C in a water-saturated atmosphere of air containing 5% CO₂. Stabilized cells (12−35th passage) were split into microtitration plates and used for further experiments.
All procedures, such as viability control (each time only cells with viability greater than 95% were used), erythrosine B staining, and light microscopy, were done in standard aseptic conditions. Each experiment for each compound was done three times in an independent triplicate.

Treml J., Leláková V., Šmejkal K., Paulíčková T., Labuda Š., Granica S., Havlík J., Jankovská D., Padrtová T, & Hošek J. (2019). Antioxidant Activity of Selected Stilbenoid Derivatives in a Cellular Model System. Biomolecules, 9(9), 468.

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Culturing cell lines for HMNJ research

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Lymphoblastoid cell lines (LCLs) were previously established from two patients with HMNJ (one male, one female) and three healthy individuals (two males, one female) following written informed consent and were cultured in Roswell Park Memorial Institute medium containing 10% FBS (fetal bovine serum), 2 mM Glutamine, 50 U/mL Penicillin and 50 mg/mL Streptomycin (Biosera, Nuaille, France).
Human SH-SY5Y and HeLa as well as mouse NSC-34 cell lines were cultured in Dulbecco's Modified Eagle's medium supplemented with 10% FBS, 2 mM Glutamine, 50 U/mL Penicillin and 50 mg/mL Streptomycin.
All cell lines were kept at 37°C in 5% CO₂.

Ververis A., Dajani R., Koutsou P., Aloqaily A., Nelson-Williams C., Loring E., Arafat A., Mubaidin A.F., Horany K., Bader M.B., Al-Baho Y., Ali B., Muhtaseb A., DeSpenza Jr T., Al-Qudah A.A., Middleton L.T., Zamba-Papanicolaou E., Lifton R, & Christodoulou K. (2019). Distal hereditary motor neuronopathy of the Jerash type is caused by a novel SIGMAR1 c.500A>T missense mutation. Journal of Medical Genetics, 57(3), 178-186.

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Culturing Human Colorectal and Endothelial Cells

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Human colorectal carcinoma cell line (DLD1; CCL-221, ATCC, USA) was cultured in RPMI GlutaMAX medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% FBS (Biosera, Nuaille, France), 100 U/mL penicillin, and 100 μg/mL streptomycin (Biosera). Human umbilical vein endothelial cells (HUVEC) were isolated from fresh umbilical cords, as previously described [24 (link)], and cultured in complete ECGM medium (PromoCell, Heidelberg, Germany) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (Biosera). Cells were cultivated at 37 °C in a humidified incubator (Heal Force, Shanghai, China) containing 5% CO₂.

Horváthová J., Moravčík R., Matúšková M., Šišovský V., Boháč A, & Zeman M. (2021). Inhibition of Glycolysis Suppresses Cell Proliferation and Tumor Progression In Vivo: Perspectives for Chronotherapy. International Journal of Molecular Sciences, 22(9), 4390.

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Culturing Human and Mouse Ovarian Cancer Cell Lines

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Human epithelial ovarian cancer cell lines SKOV3, OVCAR3, OVCAR5, A1847, A2780, and C30 were acquired from ATCC and cultured in RPMI 1640 with stable glutamine, supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Biosera). Mouse ovarian cancer cell line ID8 was originally donated by Drs. Kathy Robby and Paul Terranova and cultured in DMEM high glucose with stable glutamine and sodium pyruvate (Biosera), supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cell lines were cultured at 37°C, 5% CO₂, in a humidified atmosphere and passaged for fewer than six months since receipt and stock thawing.

Karapetsas A., Giannakakis A., Dangaj D., Lanitis E., Kynigopoulos S., Lambropoulou M., Tanyi J.L., Galanis A., Kakolyris S., Trypsianis G., Coukos G, & Sandaltzopoulos R. (2015). Overexpression of GPC6 and TMEM132D in Early Stage Ovarian Cancer Correlates with CD8+ T-Lymphocyte Infiltration and Increased Patient Survival. BioMed Research International, 2015, 712438.

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Culturing Mammalian and Leishmania Cells

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The human epithelial cell line HeLa was cultured in low-glucose DMEM (Biosera PM-D1105) containing 10% (v/v) hiFBS [heat inactivated (at 56 °C for 30 min) fetal bovine serum], 1 U/mL penicillin, and 0.1 mg/mL streptomycin. Mammalian cells were counted with a Neubauer hemocytometer (Kyrios Soter Scientific, Miami, FL 33186 USA).
L. donovani (strain LG13, MHOM/ET/0000/HUSSEN) promastigotes were cultured in RPMI 1640 containing 10% (v/v) hiFBS, 1 U/mL penicillin, 0.1 mg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), and 10 mM Hepes (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) at 25 °C, as previously described in Papadaki et al. [46 (link)]). Leishmania cells were counted with a Malassez hemocytometer. L. donovani axenic amastigotes were obtained according to a modified published protocol [46 (link)].

Tziouvara O., Petsana M., Kourounis D., Papadaki A., Basdra E., Braliou G.G, & Boleti H. (2024). Characterization of the First Secreted Sorting Nexin Identified in the Leishmania Protists. International Journal of Molecular Sciences, 25(7), 4095.

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Biological Effects of Cu(II) Complexes on Cancer Cells

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To test the biological effects of the Cu(II) complexes and the ligands on human cancer cells, DU-145 prostate, A549 lung, and MCF-7 breast adenocarcinoma cells, as well as the multidrug-resistant MCF-7 KCR breast cancer cells were utilized. All the cell lines were maintained in RPMI-1640 media (Biosera, Nuaille, France) complemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 0.01% streptomycin and 0.005% penicillin, (Biosera, Nuaille, France) and were cultured under standard conditions in a 37 °C incubator at 5% CO₂ and 95% humidity. The cell lines were originally obtained from ATCC.

Petrasheuskaya T.V., Kovács F., Igaz N., Rónavári A., Hajdu B., Bereczki L., May N.V., Spengler G., Gyurcsik B., Kiricsi M., Frank É, & Enyedy É.A. (2022). Estradiol-Based Salicylaldehyde (Thio)Semicarbazones and Their Copper Complexes with Anticancer, Antibacterial and Antioxidant Activities. Molecules, 28(1), 54.

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Isolation and Culture of Cortical Neurons

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Cortical
neurons were isolated from 14-day-old embryos of pregnant
C57BL/6JRj mice (Janvier, France). Brains and cortices were dissected,
and the olfactory bulbs and meninges were removed. The cortices cut
into small pieces were dissociated in gentleMACS tube C (Miltenyi
Biotec) using a Neural Tissue Dissociation Kit (P) (Miltenyi Biotec)
according to the manufacturer’s protocol. The dissociation
was carried out using a gentleMACS Dissociator (Miltenyi Biotec).
The dissociated tissue was filtered through 40 μm cell strainers
(BD Biosciences). Neurons were cultured in Neurobasal medium, supplemented
with GlutaMAX 0.5 mM (Gibco), B-27 supplement (Gibco), and antibiotics
[100 U mL^–1 penicillin and 100 μg mL^–1 streptomycin (Biosera, France)] in 24-well Corning poly-d-lysine BioCoat plates (Corning, MA) at a concentration of 2.5 ×
10⁵ cells/well. The next day, neuronal cultures were treated
with 5-fluoro-2′-deoxyuridine (2 μM FdU, Sigma) and uridine
(2 μM U, Sigma) to suppress the proliferation of other nonneuronal
cells potentially occurring in the cell culture. Every 3rd day, half
of the medium was replaced with fresh medium. Cortical neurons were
cultured for 12 days and then used for experiments.^{66 (link)}

Brezani V., Blondeau N., Kotouček J., Klásková E., Šmejkal K., Hošek J., Mašková E., Kulich P., Prachyawarakorn V., Heurteaux C, & Mašek J. (2024). Enhancing Solubility and Bioefficacy of Stilbenes by Liposomal Encapsulation—The Case of Macasiamenene F. ACS Omega, 9(8), 9027-9039.

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