50,000 – 100,000 cells were harvested, washed once at 300 × g for 5 min, resuspended in 200 μLof FACS Buffer, and spun onto poly-L-lysine coated microscope slides with a Shandon 4 (Thermo Scientific) cytocentrifuge at 300 rpm for 4 min. When visibly dry slides were transferred into May-Grünwald solution (Sigma-Aldrich) for 5 min, rinsed 4 times for 30 s in water, and transferred to Giemsa solution (Sigma-Aldrich) for 15 min. Slides were washed as described above, dry mounted with coverslips, and examined. All images shown were taken using a Metafer slide scanning platform and software (Metasystems) at 63X magnification.
Metafer slide scanning platform and software
Manufactured by MetaSystems
The Metafer slide scanning platform and software is a digital microscopy solution that captures high-resolution images of microscopic samples on glass slides. The system automatically scans the slides and generates digital images for analysis and review.
Automatically generated - may contain errors
3 protocols using metafer slide scanning platform and software
1
Cell Preparation for Microscopy Analysis
2
Cell Preparation for Microscopy Analysis
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50,000 – 100,000 cells were harvested, washed once at 300 × g for 5 min, resuspended in 200 μLof FACS Buffer, and spun onto poly-L-lysine coated microscope slides with a Shandon 4 (Thermo Scientific) cytocentrifuge at 300 rpm for 4 min. When visibly dry slides were transferred into May-Grünwald solution (Sigma-Aldrich) for 5 min, rinsed 4 times for 30 s in water, and transferred to Giemsa solution (Sigma-Aldrich) for 15 min. Slides were washed as described above, dry mounted with coverslips, and examined. All images shown were taken using a Metafer slide scanning platform and software (Metasystems) at 63X magnification.
3
Cell Preparation and Staining Protocol
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