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Ab199056

Manufactured by Abcam
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Ab199056 is a primary antibody that can be used in various immunoassays. It is a purified monoclonal antibody produced in mice.

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Lab products found in correlation

Ab7817, by Abcam (1 mentions) Ab76319, by Abcam (1 mentions) Ab76003, by Abcam (1 mentions) Ab270993, by Abcam (1 mentions) Ab215715, by Abcam (1 mentions) Ab5694, by Abcam (1 mentions) Ab11575, by Abcam (1 mentions) Ab75852, by Abcam (1 mentions) Ab24170, by Abcam (1 mentions) Gapdh 14c10, by Cell Signaling Technology (1 mentions) Anti ki67 b56, by BD (1 mentions) Anti egfr sc 03, by Santa Cruz Biotechnology (1 mentions) Anti tfeb a303 673a, by Fortis Life Sciences (1 mentions)

4 protocols using ab199056

1

Immunohistochemical Analysis of ECM Proteins

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For immunohistochemical analyses, the sections were exposed overnight at 4°C to antibodies against E-cadherin (rabbit monoclonal; 1 : 200; ab76319; Abcam), COL I (rabbit monoclonal; 1 : 200; ab270993; Abcam, Cambridge, UK), MMP-9 (rabbit monoclonal; 1 : 200; ab76003; Abcam), TIMP-1 (rabbit polyclonal; 1 : 200; Abcam), α-SMA (mouse monoclonal; 1 : 200; ab7817; Abcam), or fibronectin (rabbit monoclonal; 1 : 200; ab199056; Abcam), followed by incubation with secondary horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (1 : 500; 115-035-003; Jackson ImmunoResearch, Ely, UK).
Shi M., Liu H., Zhang T., Zhang M., Tang X., Zhang Z., Lu W., Yang S., Jiang Z., Cui Q, & Li Z. (2022). Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Promote Peritoneal Healing by Activating MAPK-ERK1/2 and PI3K-Akt to Alleviate Postoperative Abdominal Adhesion. Stem Cells International, 2022, 1940761.
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2

Parietal Peritoneum Morphometric Analysis

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At the end of 6 week, the rats were killed, and the parietal peritoneum of the abdominal wall was sampled for morphometric and histological analyses. The parietal peritoneum was assessed by hematoxylin and eosin (H&E) staining as previously described [17 (link)]. Fibrosis was visualized with Masson’s trichrome stain. Immunohistochemical staining experiments for α-SMA, fibronectin, and TGF-β1 were performed with rabbit polyclonal anti-α-SMA (ab5694; Abcam), rabbit monoclonal anti-fibronectin (ab199056; Abcam), and rabbit monoclonal anti-TGF-β1 [EPR21143] (ab215715; Abcam) primary antibodies, respectively.
Kang S.H., Kim S.W., Kim K.J., Cho K.H., Park J.W., Kim C.D, & Do J.Y. (2019). Effects of tranilast on the epithelial-to-mesenchymal transition in peritoneal mesothelial cells. Kidney Research and Clinical Practice, 38(4), 472-480.
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3

Antibody Immunoblotting and Immunofluorescence

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All antibodies used in this manuscript were used a 1:1000 dilution when used for immunoblotting and 1:200 when used for immunofluorescence. Antibodies against P-STAT3 (D3A7), STAT3 (124H6), P-Jak2 (D2E12), Jak2 (3771A), P-4EBP1 (236B4), LC3 (D3U4C) and GAPDH (14C10) were obtained from Cell Signaling. Antibodies against Laminin (ab11575), CtsD (ab75852), CtsB (ab92955), Lamp1 (ab24170) and Fibronectin (ab199056) were purchased from AbCam. Anti-Ki67 (B56) obtained from BD Bioscience, anti-Gns (13044-1-AP) from ProteinTech, anti-TFEB (A303-673A) from Bethyl, anti-YM1 (60130) from Stem Cell Technologies, anti-EGFR (sc-03) from SantaCruz, anti-OSMRβ (3458-100) from R&D System and anti-SOD1 (3458-100) from BioVision.
Martínez-Fábregas J., Prescott A., van Kasteren S., Pedrioli D.L., McLean I., Moles A., Reinheckel T., Poli V, & Watts C. (2018). Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis. Nature Communications, 9, 5343.
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4

Comprehensive Tumor Tissue Analysis

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Mice tumor tissues were fixed and embedded in paraffin, and sections with 10 µm thickness were prepared. For characterization of tumor hypoxia, the tumor sections were immunofluorescence stained with anti‐HIF‐1α FITC‐conjugated antibody (PTG: 20960‐1‐AP) and imaged under a confocal fluorescence microscope. For characterization of ECM, MASSON staining, Sirius red staining, collagen I (Biossci: PA1026) immunofluorescence staining, and second harmonic generation (SHG) imaging were used to detect collagen in ECM. Fibronectin (abcam: ab199056) and elastin fibers in ECM were also probed with immunofluorescence staining and Verhoeffe‐Van Gieson (VVG) staining. To evaluate the infiltration of immune cells into tumor parenchyma, CD4 (Biossci: PA1052), CD8 (Biossci: PA1050), IFN‐γ(Bioss: bs‐0480R), CD11b (Biossci: PA1045), Gr‐1 (abcam: ab238132), CD86 (CST: 19589), CD206 (Biossci: PA1052) were introduced to identify CD4+ T cells, CD8+ T cells, CTLs (CD8+IFN‐γ+), MDSCs (CD11b+Gr‐1+), M1 (CD86+), and M2 (CD206+) tumor associated macrophages by immunofluorescence staining. For quantitation of hypoxia, ECM components, and lymphocytes in tumor tissues, image pro plus software was used to analyze fluorescence signals from the staining images. At least six fields per tumor slide were counted and at least three tumor tissues per group were analyzed.
Liu X., Ye N., Liu S., Guan J., Deng Q., Zhang Z., Xiao C., Ding Z., Zhang B., Chen X., Li Z, & Yang X. (2021). Hyperbaric Oxygen Boosts PD‐1 Antibody Delivery and T Cell Infiltration for Augmented Immune Responses Against Solid Tumors. Advanced Science, 8(15), 2100233.
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