Samples for SEM analysis were processed as per the established protocol of the lab44 (link),45 (link). Briefly, hMSCs were cultured and differentiated over cover slips. These samples on coverslips were collected and fixed with Karnovsky fixative (4% paraformaldehyde and 1% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4)) for 6–8 h at 4 °C. Dried samples were mounted over aluminium stubs and sputter-coated with gold prior to imaging with EVO18 scanning electron microscope (Zeiss, Oberkochen, Germany) at 5 KVA in secondary electron imaging mode.
Evo 18 scanning electron microscope
Manufactured by Zeiss
Sourced in Germany
The EVO 18 is a scanning electron microscope (SEM) manufactured by Zeiss. It is designed for high-resolution imaging and analysis of a wide range of samples. The EVO 18 utilizes an electron beam to scan the surface of a sample, generating detailed images and providing information about its topography and composition.
Automatically generated - may contain errors
Lab products found in correlation
D8 advance x ray diffractometer, by Bruker (2 mentions)
Tensor 27, by Bruker (2 mentions)
D8 advance, by Bruker (2 mentions)
Uv 1800 spectrophotometer, by Shimadzu (1 mentions)
Nicolet 380 ft ir spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Hr2000 spectrometer, by OceanOptics (1 mentions)
Bca protein assay kit, by Meilun (1 mentions)
Smart sem v05, by Zeiss (1 mentions)
Spectrum one, by PerkinElmer (1 mentions)
Ultima 4 x ray diffractometer, by Rigaku (1 mentions)
Tensor 27 infrared spectrometer, by Bruker (1 mentions)
Sputter coater 108 auto, by Cressington (1 mentions)
Diamond tg dta, by PerkinElmer (1 mentions)
X ray photo electron spectroscopy xps, by Shimadzu (1 mentions)
Nicolet 6700 spectrophotometer, by Thermo Fisher Scientific (1 mentions)
22 protocols using evo 18 scanning electron microscope
1
Imaging hMSCs for SEM Analysis
2
Characterization of Composite Hydrogel
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An EVO-18 scanning electron microscope (SEM, Zeiss, Oberkochen, Germany) was used to investigate the morphological and structural characteristics of the hydrogel. The FT-IR spectra were recorded on a Tensor 27 (Bruker, Karlsruhe, Germany) via a KBr pellet method under dry air at room temperature; each sample was scanned from 4000 to 500 cm−1 with a resolution of 4 cm−1. X-ray diffraction (XRD) analysis of the composite hydrogel crystal structure was performed in a D8 Advance X-ray diffractometer (Bruker, Germany) using Cu Kα radiation (λ = 0.15418 nm) at 40 kV with a scanning rate of 2°/min and a scanning scope of 20−80° (2θ). UV-Vis absorption spectra were obtained for determination of optical properties in a range of 200–800 nm by a Shimadzu UV-1800 spectrophotometer. The hydrogels used for the characterizations had been lyophilized for three days at –80 °C.
3
SEM Examination of Mortar Samples
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The surface blocks of SEM test samples were treated with blank samples and coating after the mortar strength test, and then the samples were soaked in absolute ethanol for 24 h to terminate their hydration. The test instrument was a German Zeiss evo-18 scanning electron microscope.
4
Cryo-Fractured Nanocomposite SEM Imaging
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The cryo-fractured surfaces of nanocomposites were used for SEM analysis by an EVO 18 scanning electron microscope (Carl Zeiss Microscopy, GmbH, Oberkochen, Germany). The operating voltage was 20.0 kV. To avoid charging during scanning, the specimens were sputter-coated with gold prior to the experiment, and the samples were observed at a magnification of 1000×.
5
Structural, Vibrational, and Optical Characterization
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The structural information was collected using a PANalytical/X Pert powder X-ray diffractometer. The vibrational modes were examined using a Thermo Nicolet 380 FTIR spectrophotometer. The optical properties were analyzed using an Ocean optics HR2000 spectrometer. The morphology of the samples was characterized using a Carl Zeiss EVO-18 scanning electron microscope. The surface area was analyzed using a Quantachrome Nova 2200e surface area and pore size analyzer.
6
Cell Membrane Permeability and Cell Wall Solubility in Aspergillus oryzae Catalysts
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In a typical experiment, the Aspergillus oryzae whole-cell catalysts (40 mg mL−1) were incubated in different IL-containing systems at 180 rpm and 40 °C for 72 h. The supernatants were removed from the reaction mixture for measurement of protein content and polysaccharide content. The protein contents in the supernatants were used as indicator to measure the permeability of cell membrane and were detected using the BCA Protein Assay Kit purchased from Dalian Meilun Biotechnology Co., Ltd. The polysaccharide contents in the supernatants were used to measure the soluble degree of IL-containing systems to the cell wall of Aspergillus oryzae and were determined by phenol sulfuric acid method. The cells were freeze-dried in vacuum and analyzed by SEM using a ZEISS EVO 18 scanning electron microscope (ZEISS Corp., Germany). The experiments were performed triplicate. The data are presented as mean ± SD.
7
Characterization of Composite Fibers and Papers
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The morphological
and structural characteristics of the composite fibers and composite
papers were investigated using an EVO-18 scanning electron microscope
(SEM, Zeiss, Germany). The FT-IR spectra were recorded on a Tensor
27 (Bruker, Germany) under dry air at room temperature by a KBr pellet
method; each sample was scanned from 4000 to 500 cm–1 with a resolution of 4 cm–1. The composite papers
for SEM were dried at 60 °C for 6 h. X-ray diffraction (XRD)
patterns of the crystal structure were recorded on a D8 Advance X-ray
diffractometer (Bruker, Germany) using Cu Kα radiation (λ
= 0.15418 nm) at 40 kV with a scanning rate of 2°/min and a scanning
scope of 20–80° (2θ). The thermal degradation study
was carried out using a Q-500 thermogravimetric analyzer (TA) at a
nitrogen flow rate of 30 mL/min and a heating rate of 10 °C/min.
The Brunauer–Emmett–Teller (BET) surface area was determined
by N2 physisorption18 (link) using
an ASAP2020HD88 Micromeritics automated system. The paper sample was
first degassed at 115 °C for 4 h prior to the analysis by N2 adsorption at −196 °C, and BET analysis was carried
out for a relative vapor pressure of 0.01–0.3 at −196
°C.
and structural characteristics of the composite fibers and composite
papers were investigated using an EVO-18 scanning electron microscope
(SEM, Zeiss, Germany). The FT-IR spectra were recorded on a Tensor
27 (Bruker, Germany) under dry air at room temperature by a KBr pellet
method; each sample was scanned from 4000 to 500 cm–1 with a resolution of 4 cm–1. The composite papers
for SEM were dried at 60 °C for 6 h. X-ray diffraction (XRD)
patterns of the crystal structure were recorded on a D8 Advance X-ray
diffractometer (Bruker, Germany) using Cu Kα radiation (λ
= 0.15418 nm) at 40 kV with a scanning rate of 2°/min and a scanning
scope of 20–80° (2θ). The thermal degradation study
was carried out using a Q-500 thermogravimetric analyzer (TA) at a
nitrogen flow rate of 30 mL/min and a heating rate of 10 °C/min.
The Brunauer–Emmett–Teller (BET) surface area was determined
by N2 physisorption18 (link) using
an ASAP2020HD88 Micromeritics automated system. The paper sample was
first degassed at 115 °C for 4 h prior to the analysis by N2 adsorption at −196 °C, and BET analysis was carried
out for a relative vapor pressure of 0.01–0.3 at −196
°C.
8
Scanning Electron Microscopy of Fungal Conidia
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Dry conidia were transferred to an aluminum specimen mount precoated with sticky carbon conductive adhesive tape and examined with an EVO 18 scanning electron microscope (Zeiss, Oberkochen, Germany). The working temperature of the SEM stage module was − 30 to 50 °C. SEM working conditions were as follows: working distance (WD): 11 mm, electron high tension (EHT): 20.00 kV, signal A: secondary electron (SE1). Several images for each sample were digitally produced and registered at variable magnifications with the Zeiss SEM operating software Smart SEM® V05.06. The statistical analysis was done using the built-in software package in the ImageJ program.
9
Microscopic Analysis of RGLP-1 Morphology
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Morphological alterations of RGLP-1 were observed using an EVO18 scanning electron microscope (ZEISS, Germany) at an accelerating voltage of 10.0 kV. The dried sample was coated with a gold film before testing, and scanned at the magnification of 250×, 500×, 1,000×, and 2,000×.
10
Ultrastructural Analysis of Buffalo Sperm
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The buffalo sperm samples used for phagocytosis were fixed in a mixture of 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) for 12 h at 4°C. The samples were then centrifuged at 1,000 rpm for 10 min at RT and the supernatant was discarded. The pellet was suspended in 0.1 M phosphate buffer (pH 7.4) and again centrifuged at 1,000 rpm for 5 min. It was then resuspended in the buffer and a drop of it was spread on a coverslip. The samples were air-dried, sputter-coated (SCD 050 Super Cool Sputter System; Baltec Technology, Liechtenstein) with colloidal gold and observed under an EVO 18 scanning electron microscope (Carl Zeiss) at an operating voltage 20 kV. The images were digitally acquired by using the SmartSEMsoftware attached to the microscope at 5000-10000X magnification.
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