Kbf720 ich

Control and experimental flies were collected within 24 hours of eclosion, sorted by sex under carbon dioxide (CO₂) anesthesia (Genesee Scientific, USA), and maintained in a climate chamber Binder KBF720-ICH (Binder, Germany) on a food medium containing 1000 mL water, 7 g agar, 8 g yeast, 30 g sugar, 30 g semolina, and 3 mL propionic acid at constant temperature (25 °C) and humidity (60%) in a 12 hours:12 hours light:dark schedule. The flies were housed in Drosophila narrow vials (Genesee Scientific, USA) at a density of 30 individuals per vial, with 3–5 vials per experimental variant. Experiments were performed in three replicates (a total of 270–340 males and 400–500 females were analyzed). Fresh medium vials were provided two times per week. Dead flies were recorded daily. The median lifespan, the age of 90% mortality (maximum lifespan), and the mortality rate doubling time (MRDT) were calculated.

Control and experimental flies were collected during 24 h after imago hatching, sorted by sex under carbon dioxide (CO₂) anesthesia (Genesee Scientific, USA), and maintained in a constant climate chamber Binder KBF720-ICH (Binder, Germany) at 25 °C and 60% humidity in a 12:12 h light-dark cycle. The flies were housed in Drosophila narrow vials (Genesee Scientific, USA) at a density of 30 individuals per vial, with 5 vials per experimental variant. Dead flies were recorded daily. Lifespan experiments were performed in 2 replicates. The median lifespan, the age of 90% mortality (maximum lifespan), and the mortality rate doubling time (MRDT) were calculated. To compare the statistical differences in survival functions and median lifespan between control and experimental groups, the modified Kolmogorov-Smirnov and Gehan-Breslow-Wilcoxon tests were used, respectively [8 (link), 9 ]. A Wang-Allison test was used to estimate the differences in the age of 90% mortality [10 ]. Statistical analyses of the data were performed using STATISTICA software, version 6.1 (StatSoft, USA) and R, version 2.15.1.