Gsap 16kda

Proteins were extracted, sonicated, centrifuged at 13,000 rpm for 45 min at 4oC, and supernatants used for immunoblot analysis, as previously described (5 (link),6 ). Actin was always used as an internal loading control. Primary antibodies used were as follows: anti GSAP full length (1:200) and GSAP-16kDa (1:150) (Thermo Scientific, Waltham, MA); anti-TMP21 (1:200), anti-CD-147 (1:200), anti-caspase-3, anti-caspase-7 (1:200) (Santa Cruz Biotech., Dallas, TX), anti-APP (1:200), anti CTF-α and CTF-β (1:200) (Millipore, Billerica, MA, USA); anti-β-actin (1:200). IRDye infrared secondary antibodies were from LI-COR Bioscience.

Protein extracts were sonicated, centrifuged at 13,000 rpm for 45 min at 4^oC, and supernatants used for immunoblot analysis, as previously described (5 , 7 (link), 8 (link)). Actin was always used as an internal loading control. Primary antibodies used were as follows: anti GSAP full length (1:200) and GSAP-16kDa (1:150) (Thermo Scientific, Waltham, MA); anti-TMP21 (1:200) (Santa Cruz Biotech, Dallas, Texas); anti-APP (1:200), anti-APH-1 (1: 200) anti- CTF-α and anti-CTF-β(1:200) (Millipore, Billerica, MA, USA); anti-PS1 (1: 200), anti-Nicastrin (1: 200) (Cell signaling, Danvers, MA); anti-Pen-2 (1: 200) (Invitrogen, Carlsbad, CA); anti-β-actin (1:200) (Santa Cruz Biotech, Dallas, Texas). IRDye infrared secondary antibodies were from LI-COR Bioscience (Lincoln, Nebraska).