The ID8 murine ovarian surface epithelial cell line (generously donated from Drs. Paul Terranova and Kathy Roby, Kansas State University) was cultured in Dulbecco's Modified Eagles Medium (DMEM) supplemented with 10% FBS and 1% antibiotic/antimycotic (Gibco) and maintained at 37°C and 5% CO2. Human epithelial ovarian cancer cell lines OVCAR3 and CAOV3 were purchased from the American Type Culture Collection (ATCC, Manassas VA, USA) and cultured in RPMI with 20% FBS and 1% antibiotic/antimycotic (OVCAR3) or DMEM with 10% FBS and 1% antibiotic/antimycotic (CAOV3). OVCAR3(5) and CAOV3 [49 (link)] express all Akt isoforms and Akt phosphorylation is involved in proliferation in these cell lines.
Antibiotic antimycotic
Manufactured by American Type Culture Collection
Sourced in United States
Antibiotic-antimycotic is a sterile, liquid solution containing a combination of antibiotics and antifungal agents. It is designed to provide broad-spectrum antimicrobial protection in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Antibiotic antimycotic, by Thermo Fisher Scientific (22 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (9 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
L glutamine, by American Type Culture Collection (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Mem non essential amino acids solution, by Thermo Fisher Scientific (2 mentions)
Non essential amino acids (neaa), by American Type Culture Collection (2 mentions)
Htr 8 svneo, by American Type Culture Collection (2 mentions)
8 br camp, by Merck Group (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by American Type Culture Collection (2 mentions)
Ovcar3, by American Type Culture Collection (1 mentions)
Caov3, by American Type Culture Collection (1 mentions)
Wallac 1420 victor2, by PerkinElmer (1 mentions)
K sfm, by Thermo Fisher Scientific (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
30 protocols using antibiotic antimycotic
1
Culturing Ovarian Cancer Cell Lines
2
Cell Viability and Cell Cycle Assays
3
Culturing Human Cancer Cell Lines
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Human oral cancer cell line OC3-IV2 cells have been described [9 (link),25 (link),26 (link)] and were cultured in a 1:1 ratio of Dulbecco’s modified Eagle medium (DMEM; Invitrogen) and keratinocyte serum-free medium (KSFM; Invitrogen) containing 10% (v/v) fetal bovine serum (Invitrogen), 1% (v/v) l -glutamine (Invitrogen), and 1% (v/v) antibiotic-antimycotic (Invitrogen). Human primary glioblastoma cell line U87 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and grown in DMEM medium supplemented with 10% (v/v) fetal bovine serum, 1% (v/v) Minimum Essential Medium (MEM) non-essential amino acids solution (Invitrogen), and 1% (v/v) penicillin/streptomycin (Invitrogen). Human prostate cancer cell line PC3 cells, human cervical cancer cell line HeLa cells, and 293T cells were obtained from the ATCC and maintained in DMEM medium supplemented with 10% (v/v) fetal bovine serum, 1% (v/v) l -glutamine, and 1% (v/v) antibiotic-antimycotic. Human non-small cell lung carcinoma cell line H1299 cells were obtained from the ATCC and were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Invitrogen) containing 10% (v/v) fetal bovine serum, 1% (v/v) l -glutamine, and 1% (v/v) antibiotic-antimycotic. Cells were maintained in a humidified atmosphere containing 5% CO2 at 37 °C, and the culture medium was replaced every 2 days.
4
Cell Culture Protocols for HEK293, T-47D, and HCT116 Cells
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All cells were grown at 37°C, 5% CO2. HEK293 (ATCC # CRL-1573) cells were grown in DMEM media supplemented with 10% FBS, 0.01 mM non-essential amino acids (Life Technologies), 1X antibiotic-antimycotic (Life Technologies), and 1 mM sodium pyruvate (Life Technologies). T-47D (ATCC # HTB-133) cells were grown in RPMI-1640 (Hyclone) supplemented with 10% FBS, 0.01 mM non-essential amino acids, 1X antibiotic-antimycotic, and 1 mM sodium pyruvate. HCT116 (ATCC # CCL-247) cells were grown in McCoy's 5A media supplemented with 10% FBS, 0.01 mM non-essential amino acids, 1X antibiotic-antimycotic, and 1 mM sodium pyruvate. For all cell lines, the appropriate medium was refreshed every 3rd day as required, and cells were passaged 1∶10 upon reaching 80% confluence.
5
Culturing Human Cancer Cell Lines
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The Calu-3 (human lung adenocarcinoma) and SK-BR-3 (human breast adenocarcinoma) cell lines were purchased from ATCC (American Type Culture Collection, Manassas, VA, USA). The KPL4 (human breast cancer) cell line was kindly provided by Dr. Junichi Kurebayashi (Kawasaki Medical Hospital, Kurashiki, Okayama, Japan) 20 (link). The MDA-MB-231 (human breast adenocarcinoma) cell line was purchased from Perkin Elmer (Waltham, MA, USA). SK-BR-3, KPL4, and MDA-MB-231 cells were grown in RPMI-1640 medium (Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific Inc.) and 1% Antibiotic & Antimycotic ( Thermo Fisher Scientific Inc.) in a humidified atmosphere containing 5% CO2. Calu-3 cells were grown in Eagle's Minimum Essential Medium (EMEM, ATCC) supplemented with 10% fetal bovine serum and 1% Antibiotic & Antimycotic in a humidified atmosphere containing 5% CO2.
6
Detailed cell culture conditions for various cell lines
7
Maintenance and Generation of Human iPSCs
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Vero cells (CCL-81; ATCC) were maintained in Eagle’s minimum essential medium (EMEM) supplemented with 10% fetal bovine serum (FBS; HyClone) and 5% antibiotic-antimycotic (HyClone). The human iPSC lines 73-56010-02-SF and HFF1S employed in this study were generated from fibroblasts derived from skin biopsy samples which were collected from a healthy individual by the use of a 4-mm full-thickness punch biopsy while the individual was under local anesthesia. The hiPSCs were established at the National Institute of Mental Health (NIMH) Center for Collaborative Studies of Mental Disorders-funded Rutgers University Cell and DNA Repository (RUCDR; http://www.rucdr.org/mental-health ). All cells were cultured under standard conditions (37°C, 5% CO2, and 100% humidity).
8
Cell culture conditions for hematological and epithelial cell lines
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TF-1 and MV4-11 cells were obtained from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 100 units/ml of penicillin, 100 μg/ml of streptomycin sulfate, and 0.25 μg/ml of amphotericin B (Antibiotic-Antimycotic; Gibco/ThermoFisher). Parental TF-1 cells also require recombinant human GM-CSF (1 ng/mL; ThermoFisher). MOLM13 and MOLM14 cells were obtained from Leibniz Institute Deutsche Sammlung von Mikroorganismen (DSMZ) and grown in RPMI 1640 medium containing 20% FBS and Antibiotic-Antimycotic. Human 293T cells were obtained from ATCC and cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS and Antibiotic-Antimycotic.
9
Stable Cell Line Generation via Lentiviral Transduction
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HEK293T cells, 4T1.2 cells, and MDA231-BoM-1833 cells were cultured in DMEM medium supplemented with 10% FBS and 1× Antibiotic-Antimycotic (Invitrogen) at 37 °C with 5% CO2. Py8119 cells were cultured in Ham’s F12K medium containing 5% FCS (Fetal Clone II), MITO Serum Extender, and 1× Antibiotic-Antimycotic. RAW264.7 mouse macrophage cell line was from ATCC (TIB-71) and was cultured in α-MEM with 10% FBS and 1× Antibiotic-Antimycotic at 37 °C with 5% CO2. All reagents for cell culture were from Fisher Scientific. To generate stable cell lines, the pTy-U6 or pLKO.1 plasmid was cotransfected with the expression plasmids for GAG-Pol-Rev, VSV-G envelope protein, and pAdvantage into HEK293T cells using Lipofectamine 3000 (Invitrogen). The resulting virus was then filtered and used to infect 1833 cells or Py8119 cells followed by puromycin selection.
10
Malignant Melanoma Cell Lines and Growth Conditions
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Human malignant melanoma cell lines (part of NCI-60 panel of human cancer cells) - SK-MEL-5, SK-MEL-28, MALME-3M, MDA-MB-435S and normal human skin fibroblast cells MALME-3 cells were obtained from American Type Culture Collection (ATCC; Manassas, VA). SK-MEL-5 and SK-MEL-28 were grown and maintained in EMEM media (ATCC), while MALME-3M and MDA-MB-435S were grown in IMDM (ATCC) and RPMI-1640 (ATCC) respectively, as indicated by ATCC protocols. Complete growth media was supplemented with 10% fetal bovine serum (FBS; ATCC) and 1X antibiotic-antimycotic (Thermo Fisher Scientific, Waltham, MA). MALME-3 cells were grown in McCoy's medium (ATCC) supplemented with 15% FBS and 1X antibiotic-antimycotic. Cells were grown at 37C / 5% CO2 in a humidified incubator. Half the media was replaced every 48 hr. No hGH was present in the media or added externally unless specifically mentioned. For hGH treatment, 16 hr. after seeding (or 24 hr. post-transfection), the cells were serum-starved for 2 hr. in serum free growth media and hGH (PBS as control) was added at the mentioned concentrations (5, 50, 150 ng/mL). Cells were subsequently incubated for 24 hr. before RNA extraction. Recombinant hGH was purchased from Antibodies Online (Atlanta, GA).
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