Methanol

Lycopene was purchased from TRC (Toronto Research Chemicals Inc., Toronto, ON, Canada) whereas β-carotene (99.7%) was from HPC Standards GmbH (Cunnersdorf, Germany). DL-Menthol (≥95%) and lauric acid (≥98%) were from Sigma-Aldrich (St. Louis, MO, USA). Capric acid (≥98%) was from TCI (Zwijndrecht, Belgium). Thymol (ca. 98%) was from Alfa Aesar (Kandel, Germany) and ammonia solution (25%, w/v) was from ChemLab (Zedelgem, Belgium). The solvents used were of the maximum required purity. Acetonitrile (>99.9%), acetone (>99.8%) and methanol (>99.8%) (Chem-Lab, Zedelgen, Belgium) used were HPLC grade. All other reagents used were of analytical grade.

Nortriptyline and amitriptyline were purchased from Daroupakhsh Company (Tehran, Iran). Desipramine was a gift from Exir Pharmaceutical Company (Boroujerd, Iran). Clomipramine and imipramine were obtained from Soha Pharmaceutical Company (Karaj, Iran). Sodium sulfate, sodium chloride, potassium chloride, ammonia (25 %), hydrochloric acid (37 %), acetone, dimethyl formamide (DMF), sodium hydroxide, and ammonium acetate were supplied from Merck (Darmstadt, Germany). Menthol with a purity of 98 % was also purchased from Merck. Primary-secondary amine (PSA), graphitized carbon black (GCB), and octadecylsilane (C₁₈) sorbents were purchased from Agilent Technologies Company (Santa Clara, CA, USA). Methanol, acetonitrile (ACN), and HPLC-grade water were supplied from Chemlab (Zedelgem, Belgium). A mixture stock solution of drugs was prepared in Methanol with a concentration of 50 mg L^-1 (each drug) and working standard solutions were prepared daily by diluting the stock solution with HPLC-grade water.

All-trans β-carotene (for biochemistry, purity 97%) was purchased from Merck (Darmstadt, Germany). Hydroxytyrosol, tyrosol, caffeic acid, luteolin-7-O-glucoside, p-coumaric acid, and oleuropein were obtained by Extrasynthese (Genay, France). Phosphoric acid and butylated hydroxytoluene (BHT) were from Sigma-Aldrich (Steinheim, Germany). Folin–Ciocalteu reagent and HCl as well as HPLC grade acetonitrile, acetone, n-hexane, ethyl acetate, and methanol were purchased from Chem-Lab NV (Zedelgem, Belgium). D-Glucose monohydrate, casein acid hydrolysate, yeast extract, L-asparagine, KH₂PO₄, MgSO₄·7H₂O, thiamine hydrochloride as well as emulsifiers Span 20 and Tween 80 were from Scharlau Chemie S. A. (Barcelona, Spain). D-Fructose was obtained by Panreac (Barcelona, Spain), while LCK 338 cuvette test was from Hach Lange (Düsseldorf, Germany). Potato dextrose agar (PDA) was supplied by Lab M Limited (Heywood, UK). All the other reagents and solvents of appropriate grade were purchased from various producers.

Chromatographic quality organic solvents, namely acetonitrile and methanol were obtained from Chem-Lab (Zedelgem, Belgium). Chloroform, carbon tetrachloride, 1-undecanol and 1-octanol were purchased from Sigma Aldrich (St. Louis, MO, USA). The ILs 1-hexyl-3-methylimidazolium hexafluorophosphate (C₁₀H₁₉F₆N₂P) [HMIM][PF6], lithium-imide bis(trifluoromethylsulfonyl)imide (LiC₂F₆NO₄S₂) [LiTFSI] and 1-hexyl-3-methylimidazolium chloride (C₁₀H₁₉ClN₂) [HMIM Cl], were supplied by IOLITEC (Heilbronn, Germany). Other reagents, including formic acid and sodium chloride, were acquired from Sigma. Anhydrous magnesium sulfate from Análisis Vínicos (Tomelloso, Spain), hydrochloric acid from Riedel-de-Häen (Wunstarfer, Germany) and phosphoric acid from Panreac Química S.A (Barcelona, Spain) were also used. Water was previously purified using a Milli-Q system (Millipore, Bedford, MA, USA).
The cyanotoxin and phycotoxin standards were obtained from Cifga S.A. (Lugo, Spain). The standard solutions of cyanotoxins—microcystine LR (MC-LR) and RR (MC-RR) and nodularin (NOD)—were supplied at 10 µg g⁻¹, while okadaic acid (OA) was supplied at 16 µg g⁻¹ and domoic acid (DA) at 40 µg g⁻¹. All were stored in the dark at −20 °C, and standard working solutions were prepared daily diluting the toxins with ultrapure water.