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Mouse il 5 quantikine elisa kit

Manufactured by R&D Systems
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The Mouse IL-5 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure mouse Interleukin 5 (IL-5) levels in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Amicon ultra centrifugal filter unit, by Merck Group (1 mentions) Legend max mouse il 33 elisa kit, by BioLegend (1 mentions) Mouse il 13 quantikine elisa kit, by R&D Systems (1 mentions) Mouse il 2 quantikine elisa kit, by R&D Systems (1 mentions) Multiskan go, by Thermo Fisher Scientific (1 mentions) Mouse il 4 quantikine elisa kit, by R&D Systems (1 mentions) Mouse ifn γ quantikine elisa kit, by R&D Systems (1 mentions) Mouse ccl6 c c motif chemokine 6 elisa kit, by Merck Group (1 mentions)

Spelling variants of this product

Quantikine ELISA kit (1163 citations) Quantikine ELISA (398 citations) ELISAs (144 citations) Mouse ELISA kits (102 citations) Quantikine kits (80 citations) Quantikines (4 citations) IL-5 ELISA (3 citations)

4 protocols using mouse il 5 quantikine elisa kit

1

Quantifying IL-33 and IL-5 in PDAC

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ELISA was done using cell culture conditioned media from PDAC mouse cell lines and ascites fluid from PDAC tumor bearing mice. Cell culture media was concentrated using Amicon Ultra centrifugal filter units (Millipore, Z717185) before conducting ELISA. IL-33 ELISA was performed using LEGEND MAX™ Mouse IL-33 ELISA Kit (Biolegend) using manufacturer’s standard protocol. IL-5 ELISA was performed using Mouse IL-5 Quantikine ELISA Kit (R&D systems) using manufacturer’s standard protocol.
Alam A., Levanduski E., Denz P., Villavicencio H.S., Bhatta M., Alhorebi L., Zhang Y., Gomez E.C., Morreale B., Senchanthisai S., Li J., Turowski S.G., Sexton S., Sait S.J., Singh P.K., Wang J., Maitra A., Kalinski P., DePinho R.A., Wang H., Liao W., Abrams S.I., Segal B.H, & Dey P. (2022). Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer. Cancer cell, 40(2), 153-167.e11.
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2

Quantification of IL-5 and IL-13 in Colon Tissue

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The protein levels of IL-5 and IL-13 in the supernatants of the colon tissue lysates were measured using Mouse IL-13 Quantikine ELISA kit and Mouse IL-5 Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's protocol. All samples were analyzed in duplicate and normalized to the total protein content of the sample, expressed as pg/mg protein.
Wang X., Fan F, & Cao Q. (2016). Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption. Molecular Medicine Reports, 14(2), 1173-1179.
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3

Cytokine Profiling of S-2P Stimulated Splenocytes

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Two weeks after the second injection, mice were euthanized and splenocytes were isolated and stimulated with S-2P protein (2 μg/well) as previously described26 (link). For detection of IFN-γ, IL-2, IL-4, and IL-5, the culture supernatant from the 96-well microplates was harvested to analyze the levels of cytokines by ELISA using Mouse IFN-γ Quantikine ELISA Kit, Mouse IL-2 Quantikine ELISA Kit, Mouse IL-4 Quantikine ELISA Kit, and Mouse IL-5 Quantikine ELISA Kit (R&D System). The OD450 values were read by Multiskan GO (Thermo Fisher Scientific).
Kuo T.Y., Lin M.Y., Coffman R.L., Campbell J.D., Traquina P., Lin Y.J., Liu L.T., Cheng J., Wu Y.C., Wu C.C., Tang W.H., Huang C.G., Tsao K.C, & Chen C. (2020). Development of CpG-adjuvanted stable prefusion SARS-CoV-2 spike antigen as a subunit vaccine against COVID-19. Scientific Reports, 10, 20085.
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4

Quantifying Inflammatory Biomarkers in Lung Disease

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Eoxtaxin-2 levels in the BALF and IL-5 levels in the serum were respectively measured using a Mouse IL-5 Quantikine ELISA Kit (R&D Systems Inc.) and a Mouse Eotaxin-2 DuoSet ELISA Kit (R&D Systems Inc.), respectively, according to the manufacturer's instructions. IL-33 levels in the lung homogenates were measured using a Mouse IL-33 Coated ELISA Kit (Thermo Fisher Scientific Inc.). CCL-6 levels in the serum were measured using a Mouse CCL-6/C-C Motif Chemokine 6 ELISA Kit (Sigma-Aldrich, USA) according to the manufacturer's instructions.
Zhang C., Yi W., Li F., Du X., Wang H., Wu P., Peng C., Luo M., Hua W., Wong C.C., Lee J.J., Li W., Chen Z., Ying S., Ju Z, & Shen H. (2018). Eosinophil-derived CCL-6 impairs hematopoietic stem cell homeostasis. Cell Research, 28(3), 323-335.
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