PPs at 4 hours, 24 hours and 7 days were labelled with 0.5 μM BODIPY FL (Thermo Fisher, Waltham, MA), a hydrophobic dye that stains lipids, in PBS for 15 minutes at 4°C. Samples were acquired on a BD Symphony A5 flow cytometer (BD Biosciences, Franklin Lakes, NJ) with forward and side scatter in log mode and thresholding set on BODIPY-positive events. MegaMix-Plus SSC microparticles (Diagnostica Stago, Parsippany, NJ) were used to optimize scatter settings and establish size regions. Data was analysed in FlowJo 10.8 (BD Biosciences, San Jose, CA).
Megamix plus ssc microparticles
Manufactured by Diagnostica Stago
Sourced in United States
MegaMix-Plus SSC microparticles are a laboratory product manufactured by Diagnostica Stago. The core function of these microparticles is to serve as a control material for the standardization and quality control of flow cytometry analyses.
Automatically generated - may contain errors
2 protocols using megamix plus ssc microparticles
1
Lipid Staining for Flow Cytometry
2
Labeling and Flow Cytometry of PVs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PVs at 4 h, 24 h, and 7 days were labelled with 0.5 μM BODIPY FL Maleimide (B10250 Thermo Fisher, Waltham, MA, USA), a sulfhydryl-reactive hydrophobic dye, in PBS for 15 min at 4 °C. Samples were acquired on a BD Symphony A5 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) with forward and side scatter in log mode and thresholding set on BODIPY-positive events. MegaMix-Plus SSC microparticles (Diagnostica Stago, Parsippany, NJ, USA) were used to optimize scatter settings and establish size regions. Data was analysed in FlowJo 10.8 (BD Biosciences, San Jose, CA, USA).
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