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Low melting point agarose

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Low melting point agarose is a type of agarose, a polysaccharide derived from seaweed. It has a lower melting point compared to standard agarose, allowing it to solidify at lower temperatures. This property makes it suitable for applications where a softer, more fragile gel is required, such as in electrophoresis or cell culture procedures.

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Lab products found in correlation

Mivacurium chloride, by Abcam (2 mentions) U shaped glass capillary, by Leica camera (2 mentions) Ultrapure low melting point agarose, by Thermo Fisher Scientific (2 mentions) Vibratome, by Leica camera (1 mentions) Abt 263, by MedChemExpress (1 mentions) Low melting point agarose, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)

3 protocols using low melting point agarose

1

Anesthesia and Mounting Procedures for Microscopy

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Animals were either anaesthetized with 0.2 mg/ml MS-222 (PHARMAQ, UK),
or (in case of GCaMP imaging) immobilized with 0.5 mg/ml of the non-depolarizing
neuromuscular junction blocker mivacurium chloride (Abcam). For confocal
microscopy, animals were mounted laterally in 1% ultrapure low melting point
agarose (Invitrogen) on a glass coverslip. The coverslip was flipped over on a
glass slide with a ring of high-vacuum grease filled with a drop of
Danieau’s solution to prevent drying out of the agarose. For lightsheet
microscopy, embryos were mounted upright in low melting point agarose in a
U-shaped glass capillary (Leica). After imaging, the animals were either
sacrificed or released from the agarose using microsurgery blades and kept
individually until further use.
Marisca R., Hoche T., Agirre E., Hoodless L.J., Barkey W., Auer F., Castelo-Branco G, & Czopka T. (2020). Functionally Distinct Subgroups of Oligodendrocyte Precursor Cells Integrate Neural Activity and Execute Myelin Formation. Nature neuroscience, 23(3), 363-374.
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2

Anesthesia and Mounting Procedures for Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animals were either anaesthetized with 0.2 mg/ml MS-222 (PHARMAQ, UK),
or (in case of GCaMP imaging) immobilized with 0.5 mg/ml of the non-depolarizing
neuromuscular junction blocker mivacurium chloride (Abcam). For confocal
microscopy, animals were mounted laterally in 1% ultrapure low melting point
agarose (Invitrogen) on a glass coverslip. The coverslip was flipped over on a
glass slide with a ring of high-vacuum grease filled with a drop of
Danieau’s solution to prevent drying out of the agarose. For lightsheet
microscopy, embryos were mounted upright in low melting point agarose in a
U-shaped glass capillary (Leica). After imaging, the animals were either
sacrificed or released from the agarose using microsurgery blades and kept
individually until further use.
Marisca R., Hoche T., Agirre E., Hoodless L.J., Barkey W., Auer F., Castelo-Branco G, & Czopka T. (2020). Functionally Distinct Subgroups of Oligodendrocyte Precursor Cells Integrate Neural Activity and Execute Myelin Formation. Nature neuroscience, 23(3), 363-374.
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3

Preparation and Culture of Precision-Cut Lung Slices

Cited 1 time
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Healthy human lung tissue was obtained from Donor Alliance, and IPF tissue was obtained from lung biopsies or explanted lungs from confirmed cases of IPF (IRB 11-1664) as described previously (74 (link)). Tissue from patients with silicosis was obtained after lung transplant as described previously (75 (link)). For the generation of PCLS, freshly explanted healthy or IPF lungs were kept on ice and processed within 24 hours. Peripheral sections of lungs were inflated with low–melting point agarose (MilliporeSigma) by cannulation of a visible bronchus; then 6 × 6 × 6 mm cubes were cut from the lung, embedded in low–melting point agarose, and sectioned using a vibratome (Leica). We cultured 300 μm slices in Eagle minimal essential medium (EMEM, Lonza) with 10% heat-inactivated fetal bovine serum (FBS, Atlanta Biologicals) and 1% penicillin/streptomycin/l-glutamine with or without ABT-263 (5 μM, Medchemexpress) for 24 hours.
Cooley J.C., Javkhlan N., Wilson J.A., Foster D.G., Edelman B.L., Ortiz L.A., Schwartz D.A., Riches D.W, & Redente E.F. (2023). Inhibition of antiapoptotic BCL-2 proteins with ABT-263 induces fibroblast apoptosis, reversing persistent pulmonary fibrosis. JCI Insight, 8(3), e163762.
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