Anti vapb 14477 1 ap

Manufactured by Proteintech

Anti-VAPB (14477-1-AP) is a primary antibody produced by Proteintech. It is designed to detect the VAPB protein, which is involved in various cellular processes. The antibody can be used in various immunological techniques, such as western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of VAPB in biological samples.

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Lab products found in correlation

Anti gapdh sc 47724, by Santa Cruz Biotechnology (1 mentions) Anti mouse igg h l alexa fluor 488, by Abcam (1 mentions) Anti cox4 11242 1 ap, by Proteintech (1 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Bodipy 493 503, by Thermo Fisher Scientific (1 mentions) Hygromycin b, by Thermo Fisher Scientific (1 mentions) Blasticidin, by Thermo Fisher Scientific (1 mentions) Anti calnexin, by Proteintech (1 mentions) Anti mff 17090 1 ap, by Proteintech (1 mentions) Anti flag f1804, by Merck Group (1 mentions) Anti tubulin, by Proteintech (1 mentions) Anti mfn2 12186 1 ap, by Proteintech (1 mentions) Anti ptpip51 20641 1 ap, by Proteintech (1 mentions) Anti tom20 sc 17764, by Santa Cruz Biotechnology (1 mentions) Anti actin 60008 1 ig, by Proteintech (1 mentions)

Close spelling variants of this product

Anti-VAPB (4 citations) Anti-TM (2 citations)

2 protocols using anti vapb 14477 1 ap

Peroxisome Protein Localization Assay

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We used the following antibodies: anti-GAPDH (sc-47724, Santa Cruz Biotechnology), anti-PMP70 (SAB4200181, Sigma), anti-PEX19 (14713-1-AP, Proteintech), anti-PEX5 (HPA039260, Sigma), anti-VAPB (14477-1-AP, ProteinTech), anti-PEX10 (STJ119242, St John’s Laboratory), antiVAPA (sc-293278, SantaCruz Biotech) anti-PEX14 (10594-1-AP, Proteintech). Primary antibodies were used at 1:250 dilution.
Secondary antibodies for immunofluorescence: anti-rabbit IgG Cy3-conjugated (Sigma-Aldrich C2306), anti-Mouse IgG Cy3-conjugated (Sigma-Aldrich C2181), anti-rabbit IgG Cy5 conjugated (Invitrogen A10523), Anti-Mouse IgG H&L (Alexa Fluor® 488) (Abcam). Secondary antibodies were used at 1:1000 dilution.

Korotkova D., Borisyuk A., Guihur A., Bardyn M., Kuttler F., Reymond L., Schuhmacher M, & Amen T. (2024). Fluorescent fatty acid conjugates for live cell imaging of peroxisomes. Nature Communications, 15, 4314.

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Characterizing Mitochondrial Dynamics Factors

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Anti-GFP (1:1000, 50430-2-AP, ProteinTech), anti-Mff (17090-1-AP; ProteinTech), anti-Drp1 (#8570; CST), anti-Mfn2 (12186-1-AP; ProteinTech), anti-PTPIP51 (20641-1-AP; ProteinTech), anti-calnexin (10427-1-AP; ProteinTech), anti-COXIV (11242-1-AP; ProteinTech), anti-flag (F1804; Sigma), and anti-p97/VCP (10736-1-AP; ProteinTech) were used at 1:1000 dilutions for Western blots. Anti-VAPB (14477-1-AP; ProteinTech) was used at 1:1000 for Western blots and 1:200 for immunofluorescence. Anti-tom20 (sc-17764; Santa Cruz Biotechnology), anti-tubulin (11224-1-AP; ProteinTech), and anti-actin (60008-1-Ig, ProteinTech) were used at 1:5000 dilutions for Western blots. Anti-VPS13D (A304-691A; BETHY Laboratories) was used at 1:50 dilutions for coimmunoprecipitation and 1:100 for Western blots. The reagents NMS873 (TargetMol, T1853) and BODIPY 493/503 (Thermo Fisher, D3922) were used. Antibiotics such as G418 (10131027), puromycin (A1113803), hygromycin B (10687010), and blasticidin (A1113903) were obtained from Thermo Fisher (U.S.).

Du Y., Wang J., Xiong J., Fang N, & Ji W.K. (2021). VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum–mitochondria interactions. Molecular Biology of the Cell, 32(16), 1474-1486.

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