Genomic DNA was extracted from blood by standard methods and quantified. High-throughput genotyping using a pyrosequencer (Biotage AB, Uppsala, Sweden) was used for genotyping the four SNPs (rs11154801, rs7750586, rs9647635, rs9321501) of AHI1 under investigation. PCR primers (Bioneer, Daejeon, Korea) and sequencing primers (Bioneer) used for the pyrosequencing assay were designed by using the Pyrosequencing Assay Design Software v.1 (Biotage), and 1 primer of each primer set was biotinylated.
Pyrosequencing assay design software v 1
Manufactured by Biotage
The Pyrosequencing Assay Design Software v.1 is a tool that enables the design of pyrosequencing assays. It assists users in the creation of customized pyrosequencing experiments.
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Lab products found in correlation
2 protocols using pyrosequencing assay design software v 1
1
Genotyping of AHI1 Gene SNPs
2
AHI1 SNP Genotyping Protocol
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Genomic DNA was extracted from blood by standard methods and quantified. High-throughput genotyping using a pyrosequencer (Biotage AB, Sweden) was used for genotyping the four SNPs (rs11154801, rs7750586, rs9647635, rs9321501) of AHI1 under investigation. PCR primers (Bioneer, Daejeon, Korea) and sequencing primers (Bioneer) used for the pyrosequencing assay were designed by using the Pyrosequencing Assay Design Software v.1 (Biotage), and 1 primer of each primer set was biotinylated. Two independent investigators blind to the clinical status of samples checked all genotypes independently. Samples showing ambiguous alleles were discarded if they showed the same features on repeated genotyping. The final call rate was more than 99.6% for each SNP.
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