Rabbit anti phox2b

The antibodies used are: mouse anti-Meis1 (Millipore, 05–779, 1/100 for immunoblotting, Germany), goat anti-Meis1 (Santa Cruz, sc-10599, 1/100 for immunochemistry, Dallas, Texas, USA), sheep anti-TH (Thermo Scientific, PA1-4679, 1/2000, Waltham, MA, USA), rabbit anti-PGP9.5 (Millipore, AB1761, 1/500, Germany), goat anti-Nrp-1 (R&D Systems, AF566, 1/250, Minneapolis, USA), rabbit anti-Synaptophysin (Cell Signaling Technology, 5461, 1/250, Danvers, MA, USA), rabbit anti-Phox2b (kindly provided by JF Brunet, 1/250, or Abcam ab183741, 1/250, UK), goat anti-c-Ret (R&D Systems, AF482, 1/20000, Minneapolis, USA), rabbit anti-Ntrk1 (Millipore, 06–574, 1/250, Germany), rabbit anti-Cleaved Caspase-3 (Cell Signaling Technology, 9661, 1/250, Danvers, MA, USA), rabbit anti-Clathrin Heavy Chain 1 (Thermo Scientific, PA5-17347, 1/200, Waltham, MA, USA), Phospho-CREB (Cell Signaling, 87G3, 1/200, Germany), Rab5 (Abcam, ab18211, 1/200, UK), Sox2 (Santa Cruz, sc-17320, 1/250, Dallas, Texas, USA).

Immunochemical experiments were performed as previously reported^{23 (link)}. Briefly, the samples were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Industries) in phosphate-buffered saline (PBS; Thermo Fisher Scientific) for 20 min, permeabilized with 0.1% Triton X-100 (FUJIFILM Wako Pure Chemical Industries) in PBS for 10 min, and blocked with PBS containing 4% Block Ace (DS Pharma Biomedical, Osaka, Japan) and 0.1% Triton X-100 for 1 h at room temperature. The following primary antibodies were used: mouse anti-NGFR (1:200; Advanced Targeting Systems, San Diego, CA, USA), mouse anti-class III beta-tubulin (TUJ1; 1:1000; Abcam, Cambridge, UK), mouse anti-microtubule-associated protein (MAP2; 1:1000; Abcam), mouse anti-PHOX2A (1:50; Abcam), mouse anti-PHOX2B (1:100; Proteintech, Rosemont, IL, USA), mouse anti-tyrosine hydroxylase (TH; 1:200; Merck Millipore, Billerica, MA, USA), mouse anti-dopamine β-hydroxylase (DBH; 1:50; Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-phenylethanolamine N-methyltransferase (PNMT; 1:100; Abcam), rabbit anti-SOX10 (1:500; Abcam), rabbit anti-Peripherin (PRPH; 1:1000; Merck Millipore), rabbit anti-TH (1:500; Merck Millipore), rabbit anti-PHOX2B (1:100; Abcam), and rabbit anti-choline acetyltransferase (CHAT; 1:1000; Abcam).