Cd38 clone hit2

Manufactured by BioLegend

Sourced in Germany

CD38 (clone HIT2) is a monoclonal antibody that recognizes the human CD38 antigen. CD38 is a cell surface glycoprotein that functions as an ectoenzyme and mediates signaling events that influence cell activation, growth, and adhesion.

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CD38 APC-Cy7 (clone HIT2, (3 citations) Anti-human CD38(clone HIT2, (2 citations) Anti-CD38 (clone HIT2, (2 citations) CD38-PE/Cy5 (clone HIT2) (2 citations) CD38-PE/Cy7 (clone HIT2, (2 citations) PE-CD38 (clone HIT2, (2 citations)

3 protocols using cd38 clone hit2

Multiparameter Flow Cytometry of Hematopoiesis

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To observe erythroid differentiation in human tissues, staining was performed with a combination of CD117/c-Kit (clone YB5.B8; eBioscience), CD34 (clone 581; BioLegend), CD38 (clone HIT2; BioLegend), CD36 (clone 5-271; BioLegend), CD71 (clone OKT9; eBioscience), and CD235a (clone HIR2; eBioscience). To observe monocytic and granulocytic differentiation, antibodies against CD117/c-Kit, CD34, CD38, CD15 (clone HI98; eBioscience), CD14 (clone HCD14; BioLegend), and CD16 (clone 3G8; BioLegend) were used.

McKenney A.S., Lau A.N., Somasundara A.V., Spitzer B., Intlekofer A.M., Ahn J., Shank K., Rapaport F.T., Patel M.A., Papalexi E., Shih A.H., Chiu A., Freinkman E., Akbay E.A., Steadman M., Nagaraja R., Yen K., Teruya-Feldstein J., Wong K.K., Rampal R., Heiden M.G., Thompson C.B, & Levine R.L. (2018). JAK2/IDH-mutant–driven myeloproliferative neoplasm is sensitive to combined targeted inhibition. The Journal of Clinical Investigation, 128(2), 789-804.

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Multiparametric Flow Cytometry Analysis of Immune Cell Subsets

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Cells were stained using two different panels with Zombie NIR Fixable Viability stain (BioLegend, San Diego, USA) and combinations of the following fluorochrome-conjugated surface antibodies: CD4 (clone SK3), CD45 (clone HI30), CD56 (clone NCAM16.2), TCR-γ/δ (clone 11F2) all BD Biosciences, Heidelberg, Germany and CD8 (clone RPA-T8), HLA-DR (clone L243), CD45RA (clone HIT100), CD196 (CCR6) (clone G034E3), CD194 (CCR4 clone L29144), CD197 (CCR7) ((clone G043H7), CD57 (clone HNK-1), CD183 (CXCR3) (clone G025H7), CD38 (clone HIT2), CD161 (clone HP-3G10), CD25 (clone M-A251), CD3 (clone UCHT1), CD127 (clone A019D5), CD14 (clone HCD14), CD19 (clone HIB19), CD16 (clone 3G8), TCR Vα7.2 (clone 3C10), TCR Vα24-Jα18 (clone 6B11), CD314 (NKG2D) (clone 1D11), CD4 (clone SK3), TCR Vδ2-FITC, (clone B6), CD39-PE/Cy7 (clone A1) all BioLegend, San Diego, USA. Single-stained Comp Beads (Anti-Mouse Ig,κ/Negative Control Compensation Particles Set, BD Biosciences) were used for compensation. For live/dead compensation, Comp Beads stained with anti-CD14 (APC Cy-7, BioLegend) were applied. The exact composition of these two panels is displayed in S1 Table. All samples were run on a BD LSR Fortessa flow cytometer with FACS Diva version 8 (BD Biosciences) on a PC.

Diedrich T., Kummer S., Galante A., Drolz A., Schlicker V., Lohse A.W., Kluwe J., Eberhard J.M, & Schulze zur Wiesch J. (2020). Characterization of the immune cell landscape of patients with NAFLD. PLoS ONE, 15(3), e0230307.

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Multiparametric Flow Cytometry Analysis of Stimulated B Cells

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B cells were Fc blocked (BD Biosciences, San Jose, CA) prior to staining. Cells were analyzed using an LSR Fortessa cytometer (BD Biosciences), with gating performed by first setting a wide lymphocyte gate by forward and side scatter, followed by two sets of doublet exclusion, first by FSC-A by FSC-H and then by FSC-A by FSC-W, and by excluding dead cells with a viability stain (Life Technologies, Grand Island, NY). B cell purity was determined during isolation and at baseline by staining for CD19 (clone HIB19, eBiosciences, San Diego, CA). Cell phenotype was determined by staining IgM (clone MHM-88), CD27 (clone O323), and CD38 (clone HIT2, all from BioLegend, San Diego, CA), with results reported for the live cells gate. B cell proliferation was determined by dilution of CFSE dye, with results reported for the live singlet lymphocyte gate.

Simchoni N, & Cunningham-Rundles C. (2015). TLR7 and TLR9 responsive human B cells share phenotypic and genetic characteristics. Journal of immunology (Baltimore, Md. : 1950), 194(7), 3035-3044.

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