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Anti mouse tcrβ h57 597

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The Anti-mouse TCRβ (H57–597) is a laboratory reagent used for the detection and analysis of T cell receptor beta (TCRβ) chain expression in mouse cells. It is a monoclonal antibody that specifically binds to the constant region of the TCRβ chain, allowing for the identification and characterization of T cells in various research applications.

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Lab products found in correlation

Anti mouse foxp3 fjk 16s, by Thermo Fisher Scientific (3 mentions) Anti mouse ifn γ xmg1, by Thermo Fisher Scientific (3 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Zombie yellow fixable viability kit, by BioLegend (2 mentions) Anti mouse cd45 30 f11, by Thermo Fisher Scientific (2 mentions) Anti mouse il 17a tc11 18h10.1, by BioLegend (2 mentions) Anti mouse cd4 rm4 5, by Thermo Fisher Scientific (2 mentions) Ebio4b10, by Thermo Fisher Scientific (2 mentions) Golgiplug, by BD (2 mentions) Cytofix cytoperm buffer, by BD (2 mentions) Anti mouse tcr vβ screening panel, by BD (1 mentions) Zombie aqua, by BioLegend (1 mentions) Propidium iodide, by BD (1 mentions) 7 aad, by BD (1 mentions) Annexin 5, by BD (1 mentions) Anti mouse cd45 30 f11, by BioLegend (1 mentions) Anti mouse cd25 pc61, by BioLegend (1 mentions) Anti mouse ctla 4 uc10 4b9, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TCRβ (H57-597) (20 citations) H57-597 (10 citations) Anti-TCRβ (H57-597, (8 citations) Anti-TCRβ (8 citations) Anti-mouse TCRβ (2 citations) FITC-conjugated anti-mouse TCRβ (clone H57-597, (2 citations)

4 protocols using anti mouse tcrβ h57 597

1

Flow Cytometry Antibody Panel

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Antibodies used for flow cytometry were CD5 (55–7.3) (eBiosciences/ThermoFischer), CD8 (53–6.7), CD4 (RM4-5), CD44 (IM7), CD62L (MEL-14), and Annexin-V (BD Biosciences), CD3 (OKT3), CD3 (145-2C11), CD69 (H1.2F3) (BioLegend). 7-AAD, propidium iodide (BD Biosciences) or zombie aqua (BioLegend) were used for viability staining. Anti-mouse TCR-Vβ screening panel (BD Biosciences). Antibodies used for in vitro functional studies or in vivo treatment were anti-human CD3 (Clone OKT3), anti-mouse CD3ε (Clone 145-2C11) (BD Biosciences or generated in-house) and anti-mouse TCRβ (H57-597 from eBiosciences/ThermoFischer).
Crespo J., Koh Y.T., Hu N., Moore P.A., Bonvini E., Glasebrook A.L., Martin A.P, & Benschop R.J. (2021). A humanized CD3ε-knock-in mouse model for pre-clinical testing of anti-human CD3 therapy. PLoS ONE, 16(2), e0245917.
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2

Comprehensive Immune Cell Profiling

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The following monoclonal antibodies were used for cell surface staining: anti-mouse CD45 (30-F11; BioLegend), anti-mouse TCRβ (H57-597; eBioscience), anti-mouse TCRγδ (eBioGL3 (GL-3, GL3); eBioscience), anti-mouse Vγ1 (2.11; BioLegend), anti-mouse CD4 (RM4-5; BioLegend), anti-mouse CD25 (PC61; BioLegend), anti-mouse CD27 (LG.3A10; BD Biosciences), anti-mouse CD39 (Duha59; BioLegend), anti-mouse CD44 (IM7; BioLegend), anti-mouse CD62L (MEL-14; eBioscience). The following antibodies were used for intracellular staining: anti-mouse Foxp3 (FJK-16s; eBioscience), anti-RORγt (Q31-415 378; BD Biosciences), anti-mouse IL-17A (eBio17B7; eBioscience), anti-mouse IFN-γ (XMG1.2; eBioscience), anti-mouse CTLA-4 (UC10-4B9; eBioscience).
Lee W.S., Nam K.H., Kim J.H., Kim W.J., Kim J.E., Shin E.C., Kim G.R, & Choi J.M. (2023). Alleviating psoriatic skin inflammation through augmentation of Treg cells via CTLA-4 signaling peptide. Frontiers in Immunology, 14, 1233514.
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3

Cytokine Expression Analysis by Flow Cytometry

Cited 1 time
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IL-17a and IFN-γ cytokine expression were assessed by flow cytometry as described previously45 (link). Briefly, the cells were stimulated for 4 h in phorbol 1,2-myristate 1,3-acetate (5 ng/ml) and ionomycin (1 µg/ml) in the presence of protein transport inhibitor GolgiPlug (BD Pharmingen). Cells were stained for surface markers with following antibodies: anti-mouse TCRβ (H57–597; eBioscience), anti-mouse CD4 (RM4–5; eBioscience), anti-mouse CD45 (30-F11, eBioscience). Cells were washed and fixed using Cytofix/Cytoperm buffer (BD Pharmingen or eBioscience). Intracellular staining was performed with following antibodies: anti-mouse IL-17a (TC11-18H10.1; BioLegend), anti-mouse IFN-γ (XMG1.2, eBiosciences), anti-mouse Foxp3 (FJK-16s, eBioscience) and anti-human/mouse T-bet (eBio4B10, eBiosciences). Dead cells were excluded from analysis using Zombie Yellow Fixable Viability Kit (Biolegend) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies).
Placek K., Hu G., Cui K., Zhang D., Ding Y., Lee J.E., Jang Y., Wang C., Konkel J.E., Song J., Liu C., Ge K., Chen W, & Zhao K. (2017). MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping. Nature immunology, 18(9), 1035-1045.
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4

Cytokine Expression Analysis by Flow Cytometry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-17a and IFN-γ cytokine expression were assessed by flow cytometry as described previously45 (link). Briefly, the cells were stimulated for 4 h in phorbol 1,2-myristate 1,3-acetate (5 ng/ml) and ionomycin (1 µg/ml) in the presence of protein transport inhibitor GolgiPlug (BD Pharmingen). Cells were stained for surface markers with following antibodies: anti-mouse TCRβ (H57–597; eBioscience), anti-mouse CD4 (RM4–5; eBioscience), anti-mouse CD45 (30-F11, eBioscience). Cells were washed and fixed using Cytofix/Cytoperm buffer (BD Pharmingen or eBioscience). Intracellular staining was performed with following antibodies: anti-mouse IL-17a (TC11-18H10.1; BioLegend), anti-mouse IFN-γ (XMG1.2, eBiosciences), anti-mouse Foxp3 (FJK-16s, eBioscience) and anti-human/mouse T-bet (eBio4B10, eBiosciences). Dead cells were excluded from analysis using Zombie Yellow Fixable Viability Kit (Biolegend) or LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies).
Placek K., Hu G., Cui K., Zhang D., Ding Y., Lee J.E., Jang Y., Wang C., Konkel J.E., Song J., Liu C., Ge K., Chen W, & Zhao K. (2017). MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping. Nature immunology, 18(9), 1035-1045.
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