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Anti hdac2 antibody

Manufactured by Santa Cruz Biotechnology
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Sourced in United Kingdom, United States

The Anti-HDAC2 antibody is a research tool used to detect and quantify the HDAC2 protein in various biological samples. HDAC2 is a histone deacetylase enzyme that plays a role in the regulation of gene expression. The antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of HDAC2 in cells and tissues.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Anti hdac5 antibody, by Santa Cruz Biotechnology (1 mentions) Anti β actin antibody, by Abcam (1 mentions) Donkey anti goat igg hrp, by Santa Cruz Biotechnology (1 mentions) Ecl donkey anti rabbit igg hrp, by GE Healthcare (1 mentions) Anti histone h3 antibody, by Abcam (1 mentions) Anti psa antibody, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d1 antibody, by Santa Cruz Biotechnology (1 mentions) Cpi 203, by MedChemExpress (1 mentions) Anti hdac3 antibody, by Santa Cruz Biotechnology (1 mentions) Anti hdac1 antibody, by Santa Cruz Biotechnology (1 mentions) Cell counting kit 8 cck 8, by Selleck Chemicals (1 mentions) Anti ar antibody, by Santa Cruz Biotechnology (1 mentions) C myc, by Proteintech (1 mentions) Transwell chamber, by Corning (1 mentions)

Close spelling variants of this product

HDAC2 (33 citations) Anti-HDAC2 (16 citations) HDAC2 antibody (2 citations)

3 protocols using anti hdac2 antibody

1

Western Blot Analysis of HDAC2 and HDAC5

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Whole cell extracts (30 μL each) were electrophoresed and protein samples in the gels were transferred to a nitrocellulose membrane as previously shown [16 (link)]. Immunoreactive bands of HDAC2, HDAC5 and β-actin were detected with anti-HDAC2 antibody (Santa Cruz Biotechnology, Santa Cruz, CA), anti-HDAC5 antibody (Santa Cruz Biotechnology) and anti-β actin antibody (Abcam, Cambridge, UK), respectively. Band densities of HDAC2 and HDAC5 in each sample were normalized with that of the β-actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH).
To M., Swallow E.B., Akashi K., Haruki K., Natanek S.A., Polkey M.I., Ito K, & Barnes P.J. (2017). Reduced HDAC2 in skeletal muscle of COPD patients. Respiratory Research, 18, 99.
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2

Western Blot Analysis of CHD5 and HDAC2

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Cells were harvested with 0.05 % trypsin after incubation in each condition. Protein was extracted using NE-PER® Nuclear and Cytoplasmic Extraction Reagents (Thermo–Fisher Scientific; Pittsburgh, PA). SDS-PAGE and transfer were performed with Invitrogen systems (Invitrogen–Life Technologies; Grand Island, NY). Immunoblotting was performed with the following primary and secondary antibodies and dilutions: anti-CHD5 antibody (Santa-Cruz, sc-68390; Santa Cruz Biotechnology, Inc.; Dallas, TX) 1:1000, anti-HDAC2 antibody (Santa-Cruz, sc-6296) 1:1000, ECL-donkey anti-rabbit IgG-HRP (GE NA934V; GE Healthcare Life Sciences; Pittsburgh, PA) 1:2500, donkey anti-goat IgG-HRP (Santa-Cruz, sc-2020) 1:5000. For each blot, the reaction was performed at 4 °C overnight for the primary antibody and at room temperature for 2 h for the secondary antibody.
Higashi M., Kolla V., Iyer R., Naraparaju K., Zhuang T., Kolla S, & Brodeur G.M. (2015). Retinoic acid-induced CHD5 upregulation and neuronal differentiation of neuroblastoma. Molecular Cancer, 14, 150.
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3

Comprehensive Chromatin Modification Analysis

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BET762, I-BET726, and CPI-203 were purchased from MedChemExpress (NJ, USA), cell counting Kit-8 (CCK-8) was purchased from Bimake (Houston, TX, USA), and Transwell Chambers were purchased from Corning (Corning, NY, USA). Anti-histone crotonylation (Kcr) antibody, anti-histone acetylation modification (Kac) antibody, and anti-Histone H3 Lys18 crotonylation (H3K18cr) antibody at the 18th amino acid position of histone H3 were purchased from PTM-Biolabs (Hangzhou, China). Anti-actin (β-actin) antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA), anti-histone H3 antibody and BRD4 antibody were purchased from Abcam (Cambridge, UK), and anti-histone acetylase P300 antibody was purchased from AbSci (Vancouver, WA, USA). Anti-histone acetylase GCN5 antibody, anti-Cyclin D1 antibody, anti-HDAC1 antibody, anti-HDAC2 antibody, anti-HDAC3 antibody, anti-ACSS2 antibody, anti-AR antibody, and anti-PSA antibody were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and C-MYC was purchased from Proteintech.
Xu X., Zhu X., Liu F., Lu W., Wang Y, & Yu J. (2021). The effects of histone crotonylation and bromodomain protein 4 on prostate cancer cell lines. Translational Andrology and Urology, 10(2), 900-914.
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