Facs flow cytometry

Manufactured by BD

Sourced in United States, Germany

The FACS flow cytometry is a laboratory instrument that analyzes and sorts cells or particles based on their physical and chemical characteristics. It uses a laser to detect and measure the properties of individual cells or particles as they flow through a fluidic system.

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Lab products found in correlation

Propidium iodide, by Merck Group (5 mentions) Rnase a, by Merck Group (2 mentions) Pe annexin 5 apoptosis detection kit, by BD (2 mentions) Annexin 5 fitc, by Thermo Fisher Scientific (2 mentions) Annexin 5 fitc apoptosis detection kit, by Thermo Fisher Scientific (2 mentions) Annexin 5 fitc pi apoptosis detection kit, by BD (2 mentions) Propidium iodide solution, by BioLegend (1 mentions) Cd44 fitc, by BioLegend (1 mentions) Hiseq system, by Illumina (1 mentions) Cd45 pe, by BioLegend (1 mentions) Cd90 apc, by BioLegend (1 mentions) Propidium iodide, by MultiSciences Biotech (1 mentions) Annexin 5 fitc, by MultiSciences Biotech (1 mentions) Apc anti human cd3 monoclonal antibody, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Annexin 5 pi detection kit, by BD (1 mentions) Flow cytometry, by BD (1 mentions) U hplgps, by Olympus (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions)

Close spelling variants of this product

FAC Sort flow cytometry FACS Canto Flow Cytometry Analyzer FACS Vantage flow cytometry system FACS scan flow cytometry BD FACS Array flow cytometry machine FACs Fortessa Flow Cytometry system BD-FACS Celesta flow cytometry system FACS Vantage SE flow cytometry FACS Calibar flow cytometry FACS caliber flow cytometry

64 protocols using facs flow cytometry

Cell Cycle and Apoptosis Analysis via Flow Cytometry

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For cell cycle analysis, GC cells (2 × 10⁵ cells) were fixed overnight at 4 °C in 70% ethanol. Then, cells washed with PBS and treated with propidium iodide (PI, 0.05 mg/mL, Sigma, MO, USA) for 20 min at room temperature. Cell cycle analysis was used with FACS flow cytometry (BD Biosciences, NJ, USA).
Cell apoptosis was analyzed with Annexin V-FITC Apoptosis Kit (Biovision, CA, USA) according to the manufacturer’s instructions. After the labeling, Cells were analyzed with FACS flow cytometry (BD Biosciences).

Xue M., Li G., Fang X., Wang L., Jin Y, & Zhou Q. (2019). hsa_circ_0081143 promotes cisplatin resistance in gastric cancer by targeting miR-646/CDK6 pathway. Cancer Cell International, 19, 25.

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Cell Cycle and Apoptosis Analysis

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For the detection of cell cycle, the cells with pcDNA-LINC02381 were harvested after 36 h of transfection. Propidium iodide was used to stain cells, and the quantitation of cell cycle distribution was performed with FACS Flow cytometry (BD, San Diego, CA, United States). The percentage of the cells in Sub-G1, G1, S, and G2-M phases was counted and compared.
An FITC/PI apoptosis detection kit (Roche, Germany) was used to detect cell apoptosis. The cells with pcDNA-LINC02381 were harvested after 48 h of transfection and washed twice with 1× PBS. Cells were incubated with Annexin-V and PI at room temperature for 15 min in the dark. Then, samples were analyzed by a FACS Flow cytometry (BD, San Diego, CA, United States).

Jafarzadeh M, & Soltani B.M. (2020). Long Noncoding RNA LOC400043 (LINC02381) Inhibits Gastric Cancer Progression Through Regulating Wnt Signaling Pathway. Frontiers in Oncology, 10, 562253.

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Annexin V-FITC & PI Apoptosis Assay

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The apoptosis was detected by Annexin V-FITC&PI double-staining, and the cells treated with drugs in a 12-well plate were operated according to the kit instructions. Briefly, the medium and trypsin digested cells were collected simultaneously and the supernatant was centrifuged. After the cells were washed with PBS and collected by centrifugation, the mixture of Annexin V-FITC and PI staining solution was added and kept from light for 15 minutes at room temperature. The green and red fluorescence were detected by BD FACS flow cytometry (BD Biosciences, New Jersey, USA).

Wu W., Gou H., Xiang B., Geng R., Dong J., Yang X., Chen D., Dai R., Chen L, & Liu J. (2022). EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress. Journal of Oncology, 2022, 7099589.

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Evaluating Cell Cycle Dynamics

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Esophageal squamous cell carcinoma cells cultured in the presence of FTD following time courses were then harvested and fixed with 70% ice‐cold ethanol. To determine DNA content, cells were treated with ribonuclease, stained with 25 μL of propidium iodide solution (BioLegend # 421301), and finally analyzed by BD FACS flow cytometry.

Nguyen Vu T.H., Kikuchi O., Ohashi S., Saito T., Ida T., Nakai Y., Cao Y., Yamamoto Y., Kondo Y., Mitani Y., Kataoka S., Kondo T., Katada C., Yamada A., Matsubara J, & Muto M. (2023). Combination therapy with WEE1 inhibition and trifluridine/tipiracil against esophageal squamous cell carcinoma. Cancer Science, 114(12), 4664-4676.

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HUVEC Cell Binding Assay

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4 × 10⁵ HUVEC cells cultured in ECM were digested by trypsin and resuspended in PBS containing 2% FBS. Then the cells were washed and incubated with 10 μg/ml HcLc or H₃L₂ at 4 °C for 1 h, followed by FITC conjugated donkey-anti-human IgG H+L (Sangon Biotech, Shanghai, China). Cells incubated with secondary antibody were used as background fluorescence control. 1 × 10⁴ cells were analyzed in all individual detections. The binding assay was performed on a BD FACS flow cytometry and the obtained data was processed using FlowJo 7.6 software.

Jia X., Wang W., Xu Z., Wang S., Wang T., Wang M, & Wu M. (2016). A humanized anti-DLL4 antibody promotes dysfunctional angiogenesis and inhibits breast tumor growth. Scientific Reports, 6, 27985.

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Flow Cytometric Immunophenotyping of Isolated Cells

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The surface markers of isolated cells were analyzed using Phycoerythrin (PE)-conjugated antibodies, including CD34, CD45, CD73, CD90, and CD105 (eBioscence, Germany). The cells were detached by trypsin/EDTA; after which they were exposed to particular antibodies. Briefly, the detached cells were incubated with each antibody in 100 μl staining buffer containing 2% FBS/PBS for 20 min at 4°C in dark conditions. Then, the cells were washed with a staining buffer to eliminate the unattached antibodies. Finally, the cells were suspended in a staining buffer and analyzed using a flow cytometry instrument (BD FACS flow cytometry, San Joes, CA, USA). Data analysis was performed using Flowing Software 2.5.1.

Niknam B., Azizsoltani A., Heidari N., Tokhanbigli S., Alavifard H., Haji Valili M., Amani D., Asadzadeh Aghdaei H., Hashemi S.M, & Baghaei K. (2024). A Simple High Yield Technique for Isolation of Wharton’s Jelly-derived Mesenchymal Stem Cell. Avicenna Journal of Medical Biotechnology, 16(2), 95-103.

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Flow Cytometry and RNA-Seq Analysis of ASCs

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For flow cytometry and RNA-sequencing analysis, ASCL and ASCs were harvested in three passages. CD44-FITC (BioLegend, San Diego, CA, USA), CD90-APC (BioLegend), CD34 (Santa Cruz Biotechnology, Dallas, TX, USA), and CD45-PE (BioLegend) were detected by BD FACS flow cytometry (BD, Franklin Lakes, NJ, USA).
For RNA-sequencing, total RNA obtained from each sample was subjected to a sequencing library construction using the SureSelect Strand Specific RNA Library Preparation Kit for Illumina (Agilent Technologies Inc., Santa Clara, CA, USA) according to the manufacturer’s protocol. The quality of the libraries was assessed using an Agilent 2200 TapeStation D1000/High Sensitivity (Agilent Technologies). The pooled libraries of the samples were sequenced using the HiSeq system (Illumina Inc., San Diego, CA, USA) in 51-base-pair single-end reads.
For functional enrichment analysis, all differentially expressed genes (DEGs) of ASCL and ASCs were determined via|log₂ fold change| and mapped to terms in the gene ontology (GO) database (http://geneontology.org). Significantly enriched GO terms were searched for among the DEGs considering P < 0.05.

Chen K., Obara H., Matsubara Y., Fukuda K., Yagi H., Ono-Uruga Y., Matsubara K, & Kitagawa Y. (2022). Adipose-Derived Mesenchymal Stromal/Stem Cell Line Prevents Hepatic Ischemia/Reperfusion Injury in Rats by Inhibiting Inflammasome Activation. Cell Transplantation, 31, 09636897221089629.

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Annexin V-FITC and PI Apoptosis Assay

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PK-15 cells were trypsinized and collected, washed in phosphate buffered saline (PBS) twice, resuspended in 1× binding buffer, and stained with Annexin V-FITC and propidium iodide (PI) (MultiSciences, Hangzhou, China). After a 10-min incubation in the dark at room temperature, the labeled cells were quantified using FACS flow cytometry (BD Biosciences, USA) with CellQuest software within 1 h. Three independent experiments were performed.

Zhou Y.S., Gu Y.X., Qi B.Z., Zhang Y.K., Li X.L, & Fang W.H. (2017). Porcine circovirus type 2 capsid protein induces unfolded protein response with subsequent activation of apoptosis. Journal of Zhejiang University. Science. B, 18(4), 316-323.

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Quantifying Cell Death via PI-FACS

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Cells were incubated with 50 μg/ml PI (propidium iodide, Sigma) and the fluorescence of PI was measured on the FL3-H channel by FACS flow cytometry (BD) [11 (link)].

Wang P., Wang P., Liu B., Zhao J., Pang Q., Agrawal S.G., Jia L, & Liu F.T. (2015). Dynamin-related protein Drp1 is required for Bax translocation to mitochondria in response to irradiation-induced apoptosis. Oncotarget, 6(26), 22598-22612.

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Quantifying Apoptosis by FACS

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Apoptosis was determined by fluorescence-activated cell sorting (FACS) flow cytometry. The transfected cells collected after trypsinization were washed twice with PBS and resuspended in 1X binding buffer. Cells were then stained using 10 μL FITC-labeled buffer for 20 min and 10 μL PE-labeled buffer for 5 min according to the manufacturer’s instructions. The apoptosis rate was analyzed by FACS flow cytometry (BD Biosciences, Heidelberg, Germany).

Zhang Y., Liang S., Xiao B., Hu J., Pang Y., Liu Y., Yang J., Ao J., Wei L, & Luo X. (2022). MiR-323a regulates ErbB3/EGFR and blocks gefitinib resistance acquisition in colorectal cancer. Cell Death & Disease, 13(3), 256.

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