Rabbit anti gfap antibody

Cells were rinsed three times in PBS and fixed with cold methanol, washed thoroughly with PBS, incubated with DAPI staining solution (1 µg/mL, Thermo Fisher Scientific, Waltham, MA, USA) for 30 minutes (min) and rinsed three times with PBS, followed by viewing using a fluorescence microscope (Olympus, Tokyo, Japan).
For the immunofluorescent staining, the human glioma cells U87MG and M059J were cultured on the coverslips and treated with CA (25 µM, 50 µM) for 24 h, Subsequently, the cells on the coverslips were fixed with 4% paraformaldehyde (15 min at 4 °C), blocked with 1% BSA (1 h, RT), and incubated with primary antibody, rabbit anti-GFAP antibody (1:200 dilution; Proteintech, IL, USA), or mouse anti TUBB3-antibody (Tuj1, 1:200 dilution; Proteintech, IL, USA) for overnight at 4 °C. The coverslips were then washed 3 times with PBS and stained with a Cy3-conjugated anti-mouse secondary antibody or an Alexa Fluor 488-conjugated anti-rabbit secondary antibody (1:200 dilution; Thermo Fisher Scientific, Waltham, MA, USA). Cell nuclei were counterstained with DAPI (1 µg/mL, Thermo Fisher Scientific, Waltham, MA, USA). Confocal images were taken using a fluorescent microscope (Nikon Eclipse CI, Japan) and fluorescence pictures were photographed using the Nikon DS-U3 system (Japan).

Donepezil hydrochloride tablets (lot: 110822A) were purchased from Eisai China and prepared in a mixed suspension at 0.1 mg/ml. Aβ25‐35 (lot: Y‐0044) was obtained from Bioss Biotech. Streptavidin–biotin complex and terminal deoxynucleotidyl transferase dUTP nick‐end labeling (TUNEL) apoptosis detection kits were purchased from Boster Biotech. A real‐time reverse transcription–quantitative polymerase chain reaction (RT‐qPCR) kit was obtained from TransGen Biotech. Antibodies against Bcl‐2 Bax, and β‐actin were purchased from Bio‐Rad Laboratories. A brain stereotaxic apparatus (ZH‐LAN‐STAR, C type) was obtained from Huaibei Zhenhua Biological Equipment. An inverted microscope (S70) was purchased from Leica. A ChemiDoc XRS gel imaging and analysis system was obtained from Bio‐Rad Laboratories. An SP‐9000 immunohistochemistry kit was purchased from Beijing ZSGB Biological Engineering. PCR amplification instrument (TC‐512) was from Techen. Detection kits for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and ACHE were purchased from Nanjing Jiancheng Bioengineering Research Institute. Rabbit anti‐GFAP antibody (lot: 16825–1‐AP) was obtained from Proteintech. Rabbit anti‐IBA1 antibody (lot: bs‐1363R) was from Bioss Biotech.