After treatment, INS-1 cells were harvested and cell lysates were made. After concentration being measured, protein was subjected to SDS-PAGE gel under reducing condition and transferred onto a nitrocellulose membrane. After blocking with 5% milk, the membrane was incubated with anti-β actin, anti-phospho-GSK-3β (Cell signaling), anti-GSK-3β (Cell signaling), anti-SOD (Abcam), and anti-NOX4 antibodies (Abcam) at 4°C overnight. After washing, the membrane was incubated with horseradish peroxidase-conjugated secondary antibody (Jackson Labs). The reaction was visualized using an enhanced chemiluminescence system (Pierce). Immunoblots were analyzed by scanning densitometry and quantified by Quantity One gel Analysis software (Bio-Rad Laboratories).
Anti sod
Manufactured by Abcam
Anti-SOD is a lab equipment product that detects and measures superoxide dismutase (SOD) activity. It provides a quantitative assessment of SOD levels in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
Anti gsk3β, by Cell Signaling Technology (1 mentions)
Quantity one gel analysis software, by Bio-Rad (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti phospho gsk3β, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Goat anti rabbit igg, by Proteintech (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti β actin antibody, by Proteintech (1 mentions)
Anti ltb4r, by Abcam (1 mentions)
Goat anti mouse igg, by Proteintech (1 mentions)
2 protocols using anti sod
1
Immunoblot Analysis of INS-1 Cell Lysates
2
Western Blot Analysis of Lung Proteins
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Total protein from rat lung tissues or cells was extracted using RIPA lysis buffer (Beyotime). Equal amount of protein was separated by 10% SDS-PAGE gels and blotted on a PVDF membrane (Millipore). To block non-specific sites, the membranes were incubated in 5% dry milk in TBS-T saline for 2 h, the membrane immunodetected with anti-PLA2G6 (abcam), anti-LTB4R (abcam), anti-KL-6 (Bioss), anti-SOD (abcam), and anti-β-actin antibody (Proteintech) at 4°C overnight. Then membranes were washed three times with TBS-T and treated with goat anti-rabbit IgG (Proteintech) or goat anti-mouse IgG (Proteintech). Signals of membranes were measured by chemiluminescence (ECL) system, scanned and analyzed by Image Lab Software (Bio-Rad).
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